Systematic Determination of the Packaging Limit of Lentiviral Vectors
Kumar M, Keller B, Makalou N, Sutton R. Systematic Determination of the Packaging Limit of Lentiviral Vectors. Human Gene Therapy 2001, 12: 1893-1905. PMID: 11589831, DOI: 10.1089/104303401753153947.Peer-Reviewed Original ResearchMeSH KeywordsActive Transport, Cell NucleusAnimalsBlotting, SouthernCapsidCell LineDNAEnzyme-Linked Immunosorbent AssayGene Transfer TechniquesGenetic VectorsHeLa CellsHIV-1HumansLentivirusMembrane GlycoproteinsModels, GeneticPlasmidsReverse Transcriptase Polymerase Chain ReactionRNA, ViralTransduction, GeneticTransfectionViral Envelope ProteinsConceptsPackaging limitLentiviral vectorsHuman immunodeficiency virus type 1Immunodeficiency virus type 1Virus type 1Oncoretroviral vectorsTherapeutic deliveryVesicular stomatitis virus G proteinMeasurable titersLentiviral particlesLow titersViral titersType 1Great potentialForeign DNATitersProviral RNAVirus G proteinViral encapsidationHIVG proteinsLarge vectorsChromosomal DNA fragmentLimited utilityCells