2022
Cryo-EM structure of an active central apparatus
Han L, Rao Q, Yang R, Wang Y, Chai P, Xiong Y, Zhang K. Cryo-EM structure of an active central apparatus. Nature Structural & Molecular Biology 2022, 29: 472-482. PMID: 35578022, PMCID: PMC9113940, DOI: 10.1038/s41594-022-00769-9.Peer-Reviewed Original ResearchConceptsCentral apparatusDiverse cellular activitiesKinesin-like proteinCryo-EM structureArmadillo repeat proteinsCryo-electron microscopyHigh-resolution structuresEukaryotic speciesProtein subunitsMotile ciliaBridge proteinsPair of microtubulesRegulatory roleCellular activitiesProteinDynamic conformational behaviorCiliary motilityCiliaCiliary beatingStructural frameworkConformational behaviorSubunitsMicrotubulesRegulatorSpecies
2021
Cryo‐EM Structures of Outer‐arm Dynein Array Bound to Microtubule Doublet Reveal a Mechanism for Motor Coordination
Rao Q, Wang Y, Chai P, Kuo Y, Han L, Yang R, Yang Y, Howard J, Zhang K. Cryo‐EM Structures of Outer‐arm Dynein Array Bound to Microtubule Doublet Reveal a Mechanism for Motor Coordination. The FASEB Journal 2021, 35 DOI: 10.1096/fasebj.2021.35.s1.03099.Peer-Reviewed Original ResearchOuter arm dyneinMicrotubule-bound stateInner arm dyneinsCentral pair complexMicrotubule-binding domainMicrotubule doubletsIntermediate chainATP hydrolysisFundamental cellular processesHeavy chainCryo-EM structureCryo-EM analysisCryo-electron tomographyKey motor proteinCryo-electron microscopyLight chainCellular processesEukaryotic ciliaT. thermophilaEmbryonic developmentAdjacent microtubule doubletsCellular motilityMotor proteinsAxonemal dyneinsMotile cilia
2017
Cryo-EM structure of human ATR-ATRIP complex
Rao Q, Liu M, Tian Y, Wu Z, Hao Y, Song L, Qin Z, Ding C, Wang H, Wang J, Xu Y. Cryo-EM structure of human ATR-ATRIP complex. Cell Research 2017, 28: 143-156. PMID: 29271416, PMCID: PMC5799817, DOI: 10.1038/cr.2017.158.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAtaxia Telangiectasia Mutated ProteinsCarrier ProteinsChromatography, AffinityChromatography, GelCryoelectron MicroscopyDNA DamageDNA-Binding ProteinsHumansIsoxazolesMutationNuclear ProteinsPhosphorylationProtein BindingProtein Conformation, alpha-HelicalProtein FoldingProtein KinasesProtein MultimerizationPyrazinesSignal TransductionTOR Serine-Threonine KinasesConceptsATR-ATRIP complexCryo-electron microscopy structureATR protein kinasesTerminal catalytic coreCryo-EM structureDNA repair pathwaysMicroscopy structureReplication stressFunctional diversityProtein kinaseCatalytic coreRepair pathwaysStructural basisN-terminusATRIPCatalytic pocketEM mapsDistinct conformationsDNA damageConformational flexibilityInhibitory elementsATRComplex formationCritical roleComplexes