2021
The activation mechanisms of master kinases in the DNA damage response
Xiao J, Rao Q, Xu Y. The activation mechanisms of master kinases in the DNA damage response. Genome Instability & Disease 2021, 2: 211-224. DOI: 10.1007/s42764-021-00045-y.Peer-Reviewed Original ResearchDNA damage responsePIKK regulatory domainDNA-PKDamage responseMultiple DNA repair pathwaysDNA damageStructures of ATMCryo-electron microscopyDNA repair pathwaysSeries of proteinsKinase structureMaster kinaseRegulatory domainRepair pathwaysGenetic informationCritical kinaseMolecular mechanismsSophisticated mechanismsExtrinsic stimuliActivation mechanismKinaseCritical roleATRPathwayEukaryotes
2017
Cryo-EM structure of human ATR-ATRIP complex
Rao Q, Liu M, Tian Y, Wu Z, Hao Y, Song L, Qin Z, Ding C, Wang H, Wang J, Xu Y. Cryo-EM structure of human ATR-ATRIP complex. Cell Research 2017, 28: 143-156. PMID: 29271416, PMCID: PMC5799817, DOI: 10.1038/cr.2017.158.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAtaxia Telangiectasia Mutated ProteinsCarrier ProteinsChromatography, AffinityChromatography, GelCryoelectron MicroscopyDNA DamageDNA-Binding ProteinsHumansIsoxazolesMutationNuclear ProteinsPhosphorylationProtein BindingProtein Conformation, alpha-HelicalProtein FoldingProtein KinasesProtein MultimerizationPyrazinesSignal TransductionTOR Serine-Threonine KinasesConceptsATR-ATRIP complexCryo-electron microscopy structureATR protein kinasesTerminal catalytic coreCryo-EM structureDNA repair pathwaysMicroscopy structureReplication stressFunctional diversityProtein kinaseCatalytic coreRepair pathwaysStructural basisN-terminusATRIPCatalytic pocketEM mapsDistinct conformationsDNA damageConformational flexibilityInhibitory elementsATRComplex formationCritical roleComplexes