Featured Publications
Architecture of the Flaviviral Replication Complex PROTEASE, NUCLEASE, AND DETERGENTS REVEAL ENCASEMENT WITHIN DOUBLE-LAYERED MEMBRANE COMPARTMENTS*
Uchil P, Satchidanandam V. Architecture of the Flaviviral Replication Complex PROTEASE, NUCLEASE, AND DETERGENTS REVEAL ENCASEMENT WITHIN DOUBLE-LAYERED MEMBRANE COMPARTMENTS*. Journal Of Biological Chemistry 2003, 278: 24388-24398. PMID: 12700232, DOI: 10.1074/jbc.m301717200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineDetergentsEndopeptidasesFlavivirusRibonucleasesRNA, ViralVirus ReplicationConceptsViral genomic RNAVesicle packetsMembrane compartmentsGenomic RNADouble-membrane compartmentsInner membrane vesiclesViral RNA speciesHost cell membraneHeavy membrane fractionNonionic detergent extractsRNA speciesReplicative intermediate RNAViral NS3Replication complexPrior trypsin treatmentMicrococcal nucleaseComplex proteaseMembranous sitesReplicative form RNASpecialized structuresActive proteinIntermediate RNAMembrane vesiclesMembrane fractionForm RNA
2006
Nuclear Localization of Flavivirus RNA Synthesis in Infected Cells
Uchil P, Kumar A, Satchidanandam V. Nuclear Localization of Flavivirus RNA Synthesis in Infected Cells. Journal Of Virology 2006, 80: 5451-5464. PMID: 16699025, PMCID: PMC1472159, DOI: 10.1128/jvi.01982-05.Peer-Reviewed Original ResearchConceptsReplicase proteinsReplication complexMembrane-bound replication complexesRNA synthesisViral replicase proteinsRNA-dependent RNA polymerase activityViral replication complexFlavivirus RNA synthesisCell nucleus functionsRNA polymerase activityReplicase complexHost cytoplasmNuclear matrixNuclear localizationNuclear locationPeripheral laminaIsolated nucleiImmunoelectron microscopyPolymerase activityJapanese encephalitis virusFlavivirus RNAInfected cellsProteinAbsolute requirementViral RNA