Human Genome-Wide RNAi Screen Identifies an Essential Role for Inositol Pyrophosphates in Type-I Interferon Response
Pulloor NK, Nair S, McCaffrey K, Kostic AD, Bist P, Weaver JD, Riley AM, Tyagi R, Uchil PD, York JD, Snyder SH, García-Sastre A, Potter BV, Lin R, Shears SB, Xavier RJ, Krishnan MN. Human Genome-Wide RNAi Screen Identifies an Essential Role for Inositol Pyrophosphates in Type-I Interferon Response. PLOS Pathogens 2014, 10: e1003981. PMID: 24586175, PMCID: PMC3937324, DOI: 10.1371/journal.ppat.1003981.Peer-Reviewed Original ResearchConceptsInositol pyrophosphatesHuman genome-wide RNAi screenGenome-wide RNAi screenNovel regulatory proteinPattern recognition receptor RIGActivation of IRF3RNAi screenPositive regulatorTranscription factorsType I interferon responseType I interferon productionNovel regulatorRegulatory proteinsKinase activityBiochemical experimentsPhosphoryl transferIntact cellsInnate immune responseMetabolic pathwaysInterferon transcriptionGlobal regulationIRF3 activationCellular infectionPPIP5K2PPIP5K1