Analysis of Cell Viability by the MTT Assay.
Kumar P, Nagarajan A, Uchil PD. Analysis of Cell Viability by the MTT Assay. Cold Spring Harbor Protocols 2018, 2018: pdb.prot095505. PMID: 29858338, DOI: 10.1101/pdb.prot095505.Peer-Reviewed Original ResearchConceptsInsoluble purple formazanMitochondrial reductaseCell stressCell deathLive cellsDirect cell deathConversion of substrateMetabolic activityCell viabilityPurple formazanViability assaysNonadherent cellsPlate formatCellsAssaysMTT assayMTTChromogenic productSensitive assayReductaseToxic agentsPopular assayFormazanViabilityLarge changes