2000
Specificity of the Binding of Synapsin I to Src Homology 3 Domains*
Onofri F, Giovedı̀ S, Kao H, Valtorta F, Borbone L, De Camilli P, Greengard P, Benfenati F. Specificity of the Binding of Synapsin I to Src Homology 3 Domains*. Journal Of Biological Chemistry 2000, 275: 29857-29867. PMID: 10899172, DOI: 10.1074/jbc.m006018200.Peer-Reviewed Original ResearchConceptsSrc homology 3 domainSH3 domainCOOH-terminal regionSynapsin ISignal transductionC-SrcSynaptic vesicle-associated phosphoproteinsMultiple SH3 domainsGTPase-activating proteinsProtein-protein interactionsMitogen-activated protein kinaseDependent protein kinase IIProtein kinase IICytoskeleton assemblyP85 subunitDistinct binding patternsHigh-affinity interactionProtein kinaseAmphiphysin IC gammaKinase IIAlpha-spectrinGrb2SH3Synaptic vesicles
1997
Synapsin I interacts with c-Src and stimulates its tyrosine kinase activity
Onofri F, Giovedì S, Vaccaro P, Czernik A, Valtorta F, De Camilli P, Greengard P, Benfenati F. Synapsin I interacts with c-Src and stimulates its tyrosine kinase activity. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 12168-12173. PMID: 9342381, PMCID: PMC23739, DOI: 10.1073/pnas.94.22.12168.Peer-Reviewed Original ResearchConceptsTyrosine kinase activityC-SrcKinase activitySynapsin ISrc homology 3 domainNonreceptor tyrosine kinase c-SrcSynaptic vesicle-associated phosphoproteinsTyrosine kinase c-SrcSynaptic vesiclesC-Src SH3 domainKinase c-SrcEndogenous tyrosine kinase activityTotal cellular poolStoichiometric phosphorylationSynaptic vesicle preparationsCoprecipitation assaysSignal transductionProtein speciesCellular poolRegulatory sitesBinding proteinVesicle preparationsDetergent extractsSeveralfold stimulationAffinity chromatography
1996
Phosphoinositides as Regulators in Membrane Traffic
De Camilli P, Emr S, McPherson P, Novick P. Phosphoinositides as Regulators in Membrane Traffic. Science 1996, 271: 1533-1539. PMID: 8599109, DOI: 10.1126/science.271.5255.1533.Peer-Reviewed Original ResearchConceptsMembrane trafficActivation of proteinsGuanosine triphosphatasesSignal transductionVesicular transportRegulatory mechanismsPhosphatidylinositol metabolitesSecond messengerLocation signalPhosphorylated productsCell surfaceClassical rolePhosphoinositideCritical rolePhosphatidylinositolTransductionTriphosphatasesRegulatorProteinMessengerRegulationPolar headRoleRecruitmentActivation