Identification of the major structural and nonstructural proteins encoded by human parvovirus B19 and mapping of their genes by procaryotic expression of isolated genomic fragments
Cotmore S, McKie V, Anderson L, Astell C, Tattersall P. Identification of the major structural and nonstructural proteins encoded by human parvovirus B19 and mapping of their genes by procaryotic expression of isolated genomic fragments. Journal Of Virology 1986, 60: 548-557. PMID: 3021988, PMCID: PMC288924, DOI: 10.1128/jvi.60.2.548-557.1986.Peer-Reviewed Original ResearchConceptsProtein sequencesViral capsid polypeptidesCapsid polypeptidesSucrose velocity gradientsB19 genomeBacterial plasmid vectorRestriction endonuclease fragmentsGenomic fragmentProtein speciesApparent molecular weightNonstructural polypeptidesExpression constructsSimilar proteinsBAL-31GenomeExpression vectorNonstructural proteinsEndonuclease fragmentsPlasmid vectorViral genomeViral polypeptidesPolypeptide fragmentsProcaryotic expressionPolypeptideProteinThe Role of Parvovirus B19 in Aplastic Crisis and Erythema Infectiosum (Fifth Disease)
Chorba T, Coccia P, Holman R, Tattersall P, Anderson L, Sudman J, Young N, Kurczynski E, Saarinen U, Moir R, Lawrence D, Jason J, Evatt B. The Role of Parvovirus B19 in Aplastic Crisis and Erythema Infectiosum (Fifth Disease). The Journal Of Infectious Diseases 1986, 154: 383-393. PMID: 3016109, DOI: 10.1093/infdis/154.3.383.Peer-Reviewed Original ResearchConceptsErythema infectiosumAplastic crisisParvovirus B19B19-specific IgMSecondary attack rateSimultaneous outbreaksThroat garglesB19 infectionClinical entityRecent infectionAsymptomatic infectionSusceptible contactsPatientsInfectionB19Attack rateSerumComparison groupIgMHousehold membersOutbreakGargleEpidemiologyUrineNucleotide sequence and genome organization of human parvovirus B19 isolated from the serum of a child during aplastic crisis
Shade R, Blundell M, Cotmore S, Tattersall P, Astell C. Nucleotide sequence and genome organization of human parvovirus B19 isolated from the serum of a child during aplastic crisis. Journal Of Virology 1986, 58: 921-936. PMID: 3701931, PMCID: PMC253001, DOI: 10.1128/jvi.58.3.921-936.1986.Peer-Reviewed Original ResearchConceptsNucleotide sequenceLarge open reading frameMajor nonstructural proteinOpen reading frameFull-length cloneMajor structural polypeptidesGenome organizationGenomic clonesPutative polypeptideTranscription unitEntire genomeReading frameDNA sequencesFourth promoterParvovirus genomeSequence informationNonstructural proteinsGenomeTerminal repeatDependovirus genusStructural polypeptidesViral DNAB19 genomePolypeptideSequenceHuman parvovirus B19-induced epidemic acute red cell aplasia in patients with hereditary hemolytic anemia.
Saarinen U, Chorba T, Tattersall P, Young N, Anderson L, Palmer E, Coccia P. Human parvovirus B19-induced epidemic acute red cell aplasia in patients with hereditary hemolytic anemia. Blood 1986, 67: 1411-7. PMID: 3008891, DOI: 10.1182/blood.v67.5.1411.bloodjournal6751411.Peer-Reviewed Original ResearchConceptsRed cell aplasiaAcute-phase seraHuman parvovirus B19Cell aplasiaHemolytic anemiaHereditary hemolytic anemiaParvovirus B19B19-specific IgMHemoglobin SC diseaseDays of presentationB19-specific IgGParvovirus B19 DNASickle cell anemiaHuman parvovirus B19 DNAErythroid colony formationAbdominal symptomsSevere reticulocytopeniaAcute onsetAdditional patientsProdromal symptomsSC diseaseB19 DNAViremic seraPatientsCell anemia