2001
Hypermutability to ionizing radiation in mismatch repair-deficient, Pms2 knockout mice.
Xu X, Narayanan L, Dunklee B, Liskay R, Glazer P. Hypermutability to ionizing radiation in mismatch repair-deficient, Pms2 knockout mice. Cancer Research 2001, 61: 3775-80. PMID: 11325851.Peer-Reviewed Original ResearchConceptsMismatch repairSimple sequence repeatsWild-type transgenic miceCell linesLambda cII geneMutation frequencyDNA mismatch repairHigher clonogenic survivalMMR-deficient miceLambda shuttle vectorTolerance phenotypeSequence repeatsPatterns of IRReporter geneRepeat sequencesMononucleotide repeat sequencesShuttle vectorSingle bp deletionCII geneNullizygous animalsNullizygous miceHypermutabilityBp deletionWild-type miceClonogenic survival
1999
Targeted Correction of an Episomal Gene in Mammalian Cells by a Short DNA Fragment Tethered to a Triplex-forming Oligonucleotide*
Chan P, Lin M, Faruqi A, Powell J, Seidman M, Glazer P. Targeted Correction of an Episomal Gene in Mammalian Cells by a Short DNA Fragment Tethered to a Triplex-forming Oligonucleotide*. Journal Of Biological Chemistry 1999, 274: 11541-11548. PMID: 10206960, DOI: 10.1074/jbc.274.17.11541.Peer-Reviewed Original ResearchConceptsMammalian cellsGene correctionSV40 shuttle vectorTriplex motifNucleotide excision repairSequence-specific mannerEpisomal geneSupF reporter geneXPA cDNASpecific target sitesGene conversionShort DNA fragmentsDNA repairGene targetingTarget genesDNA segmentsReporter geneExcision repairDNA fragmentsBifunctional oligonucleotidesShuttle vectorSequence conversionGenesSV40 vectorsFlexible linker
1996
Other transgenic mutation assays: Tissue specificity of spontaneous point mutations in λsupF transgenic mice
Leach E, Narayanan L, Havre P, Gunther E, Yeasky T, Glazer P. Other transgenic mutation assays: Tissue specificity of spontaneous point mutations in λsupF transgenic mice. Environmental And Molecular Mutagenesis 1996, 28: 459-464. PMID: 8991078, DOI: 10.1002/(sici)1098-2280(1996)28:4<459::aid-em23>3.0.co;2-d.Peer-Reviewed Original Research
1995
Altered Repair of Targeted Psoralen Photoadducts in the Context of an Oligonucleotide-mediated Triple Helix (∗)
Wang G, Glazer P. Altered Repair of Targeted Psoralen Photoadducts in the Context of an Oligonucleotide-mediated Triple Helix (∗). Journal Of Biological Chemistry 1995, 270: 22595-22601. PMID: 7673252, DOI: 10.1074/jbc.270.38.22595.Peer-Reviewed Original ResearchConceptsTriplex-forming oligonucleotidesMammalian cellsPattern of mutationsSV40 shuttle vectorHeLa whole cell extractsMonkey COS cellsDNA repair pathwaysNucleotide excision repairSequence-specific mannerWhole cell extractsExcision repair patchesTriple helixPsoralen adductsSite-specific modificationThird strandGenetic manipulationSame target siteRepair pathwaysCOS cellsExcision repairAltered repairShuttle vectorCell extractsIncision stepTarget siteMutagenesis by 8-methoxypsoralen and 5-methylangelicin photoadducts in mouse fibroblasts: mutations at cross-linkable sites induced by offoadducts as well as cross-links.
Gunther E, Yeasky T, Gasparro F, Glazer P. Mutagenesis by 8-methoxypsoralen and 5-methylangelicin photoadducts in mouse fibroblasts: mutations at cross-linkable sites induced by offoadducts as well as cross-links. Cancer Research 1995, 55: 1283-8. PMID: 7882323.Peer-Reviewed Original ResearchConceptsLambda phage shuttle vectorMutation reporter geneMammalian cellsCross-linkable sitesFibroblast cell lineMouse fibroblast cell lineReporter geneMolecular eventsShuttle vectorSpectrum of mutationsMouse fibroblastsPremutagenic lesionsSupF geneCross-link formationMutationsC transversionCell linesMutagenesisGenesApt sitePsoralen treatmentCellsSitesDNAFrequent T:A→G:C transversions in X-irradiated mouse cells
Yuan J, Yeasky T, Rhee M, Glazer P. Frequent T:A→G:C transversions in X-irradiated mouse cells. Carcinogenesis 1995, 16: 83-88. PMID: 7834808, DOI: 10.1093/carcin/16.1.83.Peer-Reviewed Original ResearchConceptsMammalian cellsMouse cellsC transversionRadiation-induced point mutationsLambda phage shuttle vectorPoint mutationsMutation reporter geneRecoverable lambda phage shuttle vectorDistinctive mutational signaturesUnique mutational spectrumPattern of mutationsReporter geneSpontaneous mutationsShuttle vectorCellular repair processesMultiple copiesMutational signaturesPolymerase betaMouse fibroblastsMutationsMutational spectrumTransversionsRepair processCellsPossible role
1989
Lambda phage shuttle vectors for analysis of mutations in mammalian cells in culture and in transgenic mice
Summers W, Glazer P, Malkevich D. Lambda phage shuttle vectors for analysis of mutations in mammalian cells in culture and in transgenic mice. Mutation Research/Fundamental And Molecular Mechanisms Of Mutagenesis 1989, 220: 263-268. PMID: 2522589, DOI: 10.1016/0165-1110(89)90030-4.Peer-Reviewed Original ResearchConceptsMammalian DNALambda phage shuttle vectorForeign DNA sequencesLambda packaging extractsAnalysis of mutationsInfectious phage particlesMammalian cellsBacteriophage genomesGene sequencesDNA sequencesChromosomal sequencesBacterial systemsMammalian mutagenesisShuttle vectorLambda phageMolecular levelPhage particlesE. coliDNATransgenic miceSequenceMutationsGenomeMutagenesisPhages
1986
Detection and analysis of UV-induced mutations in mammalian cell DNA using a lambda phage shuttle vector.
Glazer P, Sarkar S, Summers W. Detection and analysis of UV-induced mutations in mammalian cell DNA using a lambda phage shuttle vector. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 1041-1044. PMID: 2937054, PMCID: PMC323006, DOI: 10.1073/pnas.83.4.1041.Peer-Reviewed Original ResearchConceptsMammalian cellsStable mouse L cell lineLambda phage shuttle vectorMouse cell DNACell DNAMammalian cell DNASupF geneMouse L cell lineDNA sequence analysisLambda phage vectorL cell lineMutant phageMouse cellsSequence analysisShuttle vectorUV-induced mutationsMultiple copiesUV mutagenesisPhage vectorEscherichia coliMutagenesisViable phagePhagesGenesT transition
1985
Direct and inducible mutagenesis in mammalian cells.
Summers W, Sarkar S, Glazer P. Direct and inducible mutagenesis in mammalian cells. Cancer Surveys 1985, 4: 517-28. PMID: 3916654.Peer-Reviewed Original ResearchConceptsMammalian cellsAnimal cellsAnimal virusesSimple eukaryotesInducible mutagenesisMutant geneSequence analysisShuttle vectorMutagenic pathwayMutagenesisDNA damageViral genomeWeigle mutagenesisMutagenic mechanismsDirect sequence analysisProkaryotesCellsBacteriaEukaryotesGenomeSimilar sitesRecent dataGenesExtrapolation of resultsPhages