2006
The life-extending gene Indy encodes an exchanger for Krebs-cycle intermediates
Knauf F, Mohebbi N, Teichert C, Herold D, Rogina B, Helfand S, Gollasch M, Luft FC, Aronson PS. The life-extending gene Indy encodes an exchanger for Krebs-cycle intermediates. Biochemical Journal 2006, 397: 25-29. PMID: 16608441, PMCID: PMC1479758, DOI: 10.1042/bj20060409.Peer-Reviewed Original ResearchConceptsExternal citrateKrebs cycle intermediatesXenopus oocytesSodium dicarboxylate cotransporterMammalian genesDrosophila melanogasterIndy mutantsLifespan extensionLongevity genesPlasma membraneSubstrate effluxExternal mediumSpecific transport mechanismsFunctional studiesSuccinate uptakeAddition of succinateEnergy metabolismIndyTransportable substratesGenesOocytesEffluxMelanogasterMutantsSuccinate
2002
Renal expression of novel Na+/H+exchanger isoform NHE8
Goyal S, Heuvel G, Aronson PS. Renal expression of novel Na+/H+exchanger isoform NHE8. American Journal Of Physiology. Renal Physiology 2002, 284: f467-f473. PMID: 12409279, DOI: 10.1152/ajprenal.00352.2002.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibody SpecificityCation Transport ProteinsCOS CellsDrosophila ProteinsHumansImmunoblottingIn Situ HybridizationKidneyMembrane ProteinsMiceMicrovilliMolecular Sequence DataOrgan SpecificityProtein IsoformsRatsRNA, MessengerSequence AlignmentSequence Analysis, DNASequence Homology, Amino AcidSodium-Hydrogen Exchanger 1Sodium-Hydrogen ExchangersTransfectionConceptsProximal tubulesComplete open reading frameProximal tubule localizationRat renal cortexAmino acid identityOpen reading frameRenal expressionCortical tubulesRenal cortexOuter stripeOuter medullaNull miceMammalian orthologsWestern blotDrosophila melanogasterMouse kidneyMedullaMembrane ion transportProtein expressionAcid identityNHE8CortexMammalian isoformsReading frameNHE isoformsFunctional characterization and immunolocalization of the transporter encoded by the life-extending gene Indy
Knauf F, Rogina B, Jiang Z, Aronson PS, Helfand SL. Functional characterization and immunolocalization of the transporter encoded by the life-extending gene Indy. Proceedings Of The National Academy Of Sciences Of The United States Of America 2002, 99: 14315-14319. PMID: 12391301, PMCID: PMC137881, DOI: 10.1073/pnas.222531899.Peer-Reviewed Original ResearchConceptsPlasma membraneLife-extending mutationsNew longevity genesIntermediary metabolismAdult fat bodyIndy expressionCitric acid cycleLife-extending effectsMetabolite transportersVariety of speciesLongevity genesFunctional characterizationLife spanMidgut cellsFat bodyAcid cycleFunctional studiesXenopus oocytesTransport functionIndyBasolateral regionSodium-independent mechanismProminent expressionAverage life spanTransporters