2004
Role of dipeptidyl peptidase IV in regulating activity of Na+/H+ exchanger isoform NHE3 in proximal tubule cells
Girardi AC, Knauf F, Demuth HU, Aronson PS. Role of dipeptidyl peptidase IV in regulating activity of Na+/H+ exchanger isoform NHE3 in proximal tubule cells. American Journal Of Physiology - Cell Physiology 2004, 287: c1238-c1245. PMID: 15213057, DOI: 10.1152/ajpcell.00186.2004.Peer-Reviewed Original ResearchConceptsProximal tubule cellsDPPIV inhibitorsDipeptidyl peptidase IVTubule cellsNHE3 activityOpossum kidney proximal tubule cellsKidney proximal tubule cellsNHE3 surface expressionPeptidase IVAdditive inhibitory effectEffects of genisteinForskolin/IBMXTyrosine kinase inhibitor genisteinSpecific competitive inhibitorPKA inhibitor HKinase inhibitor genisteinP32/98Activation of PKAInhibitory effectRenal brush border membraneBrush border membraneSurface expressionProtein kinase pathwayNHE3Inhibitor genistein
2001
Association of Na+-H+ Exchanger Isoform NHE3 and Dipeptidyl Peptidase IV in the Renal Proximal Tubule*
Girardi A, Degray B, Nagy T, Biemesderfer D, Aronson P. Association of Na+-H+ Exchanger Isoform NHE3 and Dipeptidyl Peptidase IV in the Renal Proximal Tubule*. Journal Of Biological Chemistry 2001, 276: 46671-46677. PMID: 11590171, DOI: 10.1074/jbc.m106897200.Peer-Reviewed Original ResearchConceptsApical membraneExchanger Isoform NHE3Dipeptidyl peptidase IVN-terminal sequencingProtein complexesDPPIV enzyme activityRenal microvillus membrane vesiclesMicrovillar fractionMembrane populationsMembrane vesiclesPeptidase IVWestern blot analysisMicrovillus membrane vesiclesNHE3 surface expressionBrush borderImmunoelectron microscopyRabbit renal brush borderBlot analysisProteinEnzyme activitySurface expressionMicrovillus membraneNHE3Different poolsImmunoaffinity chromatography