2005
Role of PDZK1 in membrane expression of renal brush border ion exchangers
Thomson RB, Wang T, Thomson BR, Tarrats L, Girardi A, Mentone S, Soleimani M, Kocher O, Aronson PS. Role of PDZK1 in membrane expression of renal brush border ion exchangers. Proceedings Of The National Academy Of Sciences Of The United States Of America 2005, 102: 13331-13336. PMID: 16141316, PMCID: PMC1201624, DOI: 10.1073/pnas.0506578102.Peer-Reviewed Original ResearchConceptsProximal tubulesExpression of NHE3Functional activityRole of PDZK1Protein PDZK1Reabsorption of NaBrush border expressionMammalian kidneyCFEXBrush border localizationMutant miceGST fusion proteinProtein expressionKidneyBrush border membrane proteinsExchanger NHE3Membrane expressionNHE3Brush borderBrush border membrane vesiclesPDZK1 interactionTubulesNormal expressionMembrane proteinsPDZK1
2001
Identification of a chloride-formate exchanger expressed on the brush border membrane of renal proximal tubule cells
Knauf F, Yang C, Thomson R, Mentone S, Giebisch G, Aronson P. Identification of a chloride-formate exchanger expressed on the brush border membrane of renal proximal tubule cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 2001, 98: 9425-9430. PMID: 11459928, PMCID: PMC55437, DOI: 10.1073/pnas.141241098.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAmino Acid SequenceAnimalsAntiportersBase SequenceCloning, MolecularCOS CellsDNA PrimersDNA, ComplementaryFemaleKidney Tubules, ProximalMiceMice, Inbred C57BLMicroscopy, FluorescenceMicrovilliMolecular Sequence DataSodium-Phosphate Cotransporter Proteins, Type IIaXenopus laevis
1999
Ksp-cadherin gene promoter. I. Characterization and renal epithelial cell-specific activity
Whyte D, Li C, Thomson R, Nix S, Zanjani R, Karp S, Aronson P, Igarashi P. Ksp-cadherin gene promoter. I. Characterization and renal epithelial cell-specific activity. American Journal Of Physiology 1999, 277: f587-f598. PMID: 10516284, DOI: 10.1152/ajprenal.1999.277.4.f587.Peer-Reviewed Original ResearchConceptsHepatocyte nuclear factor 3Activator protein-2Renal epithelial cellsCAAT boxNuclear proteinsFlanking regionsPromoter activityTissue-specific gene expressionEpithelial cellsElectrophoretic mobility shift assaysKsp-cadherin gene promoterCCAAT/enhancer-binding proteinTranscription initiation siteKidney-specific memberMobility shift assaysConsensus promoter elementsEnhancer-binding proteinCell-specific activityPotential binding sitesLambda bacteriophage clonesLuciferase reporter plasmidHelix proteinsKidney epithelial cellsGATA factorsGC box
1995
Isolation and cDNA Cloning of Ksp-cadherin, a Novel Kidney-specific Member of the Cadherin Multigene Family *
Thomson R, Igarashi P, Biemesderfer D, Kim R, Abu-Alfa A, Soleimani M, Aronson P. Isolation and cDNA Cloning of Ksp-cadherin, a Novel Kidney-specific Member of the Cadherin Multigene Family *. Journal Of Biological Chemistry 1995, 270: 17594-17601. PMID: 7615566, DOI: 10.1074/jbc.270.29.17594.Peer-Reviewed Original ResearchConceptsKidney-specific memberMultigene familyKsp-cadherinCDNA cloningCharacteristic cysteine residuesT7 expression systemClassical cadherinsCytoplasmic domainCysteine residuesExpression systemTissue developmentImmunolocalization studiesRecognition sequenceNorthern analysisCadherinDemonstrated roleCellular recognitionMolecular analysisLI-cadherinHPT-1Basolateral membraneCloningRenal tubular epithelial cellsEpithelial cellsProtein
1993
NHE3: a Na+/H+ exchanger isoform of renal brush border
Biemesderfer D, Pizzonia J, Abu-Alfa A, Exner M, Reilly R, Igarashi P, Aronson PS. NHE3: a Na+/H+ exchanger isoform of renal brush border. American Journal Of Physiology 1993, 265: f736-f742. PMID: 8238556, DOI: 10.1152/ajprenal.1993.265.5.f736.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceDNA PrimersFluorescent Antibody TechniqueImmunoblottingImmunohistochemistryKidneyKidney CortexKidney Tubules, ProximalL CellsMiceMicrovilliMolecular Sequence DataMolecular WeightPolymerase Chain ReactionRabbitsRecombinant ProteinsSodium-Hydrogen ExchangersTransfectionConceptsBrush borderRabbit kidneyBrush border membraneProximal tubule cellsRenal brush borderRenal proximal tubulesWestern blot analysisImmune serumProximal tubulesTubule cellsGuinea pigsImmunocytochemical studyRenal brush border membraneKidneyTranscellular reabsorptionExchanger isoformsNHE3Blot analysisFusion proteinSpecific reactivityBasolateral membraneExchanger geneCellsProtein
1992
cDNA cloning and localization of a band 3-related protein from ileum
Chow A, Dobbins JW, Aronson PS, Igarashi P. cDNA cloning and localization of a band 3-related protein from ileum. American Journal Of Physiology 1992, 263: g345-g352. PMID: 1415547, DOI: 10.1152/ajpgi.1992.263.3.g345.Peer-Reviewed Original ResearchConceptsRabbit ileal enterocytesDeduced amino acid sequenceBand 3Anion exchanger familyAmino acid sequenceAE gene familyBrush border membraneGene familyNonerythroid tissuesHCO3- exchangerErythroid band 3CDNA cloningPlasma membraneAcid sequenceExchanger familyRelated proteinsMembrane vesiclesIleal enterocytesMolecular identityCytoplasmic fragmentsProteinBasolateral membrane vesiclesBasolateral membraneMembraneEnterocytes
1991
cDNA cloning and immunolocalization of a Na(+)-H+ exchanger in LLC-PK1 renal epithelial cells
Reilly RF, Hildebrandt F, Biemesderfer D, Sardet C, Pouyssegur J, Aronson PS, Slayman CW, Igarashi P. cDNA cloning and immunolocalization of a Na(+)-H+ exchanger in LLC-PK1 renal epithelial cells. American Journal Of Physiology 1991, 261: f1088-f1094. PMID: 1661081, DOI: 10.1152/ajprenal.1991.261.6.f1088.Peer-Reviewed Original ResearchConceptsLLC-PK1 cellsFusion proteinAmino-terminal hydrophobic regionLLC-PK1 renal epithelial cellsHydrophobic regionAmino acid sequenceConfluent LLC-PK1 cellsRelative molecular massRenal epithelial cellsSignificant homologyHuman proteinsCDNA cloningAcid sequenceNucleotide identityHuman sequenceLibrary screeningCDNAMolecular massPolymerase chain reactionAmino acidsApical membraneProteinConfocal microscopyHydrophilic domainsLLC-PK1Cloning, sequence, and tissue distribution of a rabbit renal Na+/H+ exchanger transcript
Hildebrandt F, Pizzonia J, Reilly R, Rebouças N, Sardet C, Pouysségur J, Slayman C, Aronson P, Igarashi P. Cloning, sequence, and tissue distribution of a rabbit renal Na+/H+ exchanger transcript. Biochimica Et Biophysica Acta 1991, 1129: 105-108. PMID: 1661611, DOI: 10.1016/0167-4781(91)90221-7.Peer-Reviewed Original Research