2007
NHE3 phosphorylation at serines 552 and 605 does not directly affect NHE3 activity
Kocinsky HS, Dynia DW, Wang T, Aronson PS. NHE3 phosphorylation at serines 552 and 605 does not directly affect NHE3 activity. American Journal Of Physiology. Renal Physiology 2007, 293: f212-f218. PMID: 17409282, DOI: 10.1152/ajprenal.00042.2007.Peer-Reviewed Original ResearchMeSH Keywords1-Methyl-3-isobutylxanthineAnimalsCells, CulturedColforsinCyclic AMP-Dependent Protein KinasesElectrophoresis, Polyacrylamide GelKidneyKidney Tubules, ProximalMaleMicrovilliParathyroid HormonePhosphodiesterase InhibitorsPhosphorylationRatsRats, Sprague-DawleySerineSodiumSodium RadioisotopesSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersStimulation, ChemicalConceptsSerine 552NHE3 phosphorylationNHE3 activityMicrovillar membrane vesiclesPhosphorylation of NHE3Direct phosphorylationPhosphospecific antibodiesSprague-Dawley ratsExchanger type 3PKA activationMembrane vesiclesSite-specific changesPhosphorylationTransport activityOpossum kidney cellsParathyroid hormoneIntravenous infusionRat modelExchange activityKidney cellsOKP cellsNHE3 inhibitionPKACell modelExact role
2005
Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites
Kocinsky HS, Girardi AC, Biemesderfer D, Nguyen T, Mentone S, Orlowski J, Aronson PS. Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites. American Journal Of Physiology. Renal Physiology 2005, 289: f249-f258. PMID: 15687252, DOI: 10.1152/ajprenal.00082.2004.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodiesAntibodies, MonoclonalAntibody SpecificityCell LineChlorocebus aethiopsCOS CellsCyclic AMP-Dependent Protein KinasesDopamineElectrophoresis, Polyacrylamide GelImmunohistochemistryKidneyMaleMiceMice, Inbred BALB CMicroscopy, FluorescenceMicrovilliOpossumsPhosphorylationRatsRats, Sprague-DawleySodiumSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSubcellular FractionsTransfectionConceptsSerine 552Total NHE3Brush border membranePKA consensus sitesEffects of dopamineProximal tubule cellsWestern blot assaysOpossum kidney cellsBaseline phosphorylationPeptide antibodiesInhibition of NHE3Tubule cellsBlot assaysAntibodiesPhosphorylation of NHE3Phosphospecific antibodiesExchanger NHE3NHE3DopamineEndogenous NHE3Kidney cellsVivoPhospho-specific antibodiesTransfection studiesVitro