1998
cDNA Cloning and Chromosomal Localization of the Human and Mouse Isoforms of Ksp-Cadherin
Thomson RB, Ward DC, Quaggin SE, Igarashi P, Muckler ZE, Aronson PS. cDNA Cloning and Chromosomal Localization of the Human and Mouse Isoforms of Ksp-Cadherin. Genomics 1998, 51: 445-451. PMID: 9721215, DOI: 10.1006/geno.1998.5402.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCadherinsCell Adhesion MoleculesChromosome MappingChromosomes, Human, Pair 16Cloning, MolecularHumansIn Situ Hybridization, FluorescenceKidneyMiceMice, Inbred StrainsMolecular Sequence DataMultigene FamilyRNA, MessengerSequence Analysis, DNASequence Homology, Amino AcidConceptsMouse isoformsKsp-cadherinPutative N-glycosylation sitesInferred amino acid sequenceSingle ancestral geneCharacteristic protein domainsCadherin family membersKidney-specific memberN-glycosylation sitesAmino acid sequenceMammalian genomesAncestral geneGene duplicationSyntenic regionsChromosomal localizationComplete cDNAGene mapsProtein domainsSequence motifsGene sequencesCDNA cloningCell adhesion moleculeAcid sequenceNorthern analysisChromosome 8
1995
Isolation and cDNA Cloning of Ksp-cadherin, a Novel Kidney-specific Member of the Cadherin Multigene Family *
Thomson R, Igarashi P, Biemesderfer D, Kim R, Abu-Alfa A, Soleimani M, Aronson P. Isolation and cDNA Cloning of Ksp-cadherin, a Novel Kidney-specific Member of the Cadherin Multigene Family *. Journal Of Biological Chemistry 1995, 270: 17594-17601. PMID: 7615566, DOI: 10.1074/jbc.270.29.17594.Peer-Reviewed Original ResearchConceptsKidney-specific memberMultigene familyKsp-cadherinCDNA cloningCharacteristic cysteine residuesT7 expression systemClassical cadherinsCytoplasmic domainCysteine residuesExpression systemTissue developmentImmunolocalization studiesRecognition sequenceNorthern analysisCadherinDemonstrated roleCellular recognitionMolecular analysisLI-cadherinHPT-1Basolateral membraneCloningRenal tubular epithelial cellsEpithelial cellsProtein
1992
cDNA cloning and localization of a band 3-related protein from ileum
Chow A, Dobbins JW, Aronson PS, Igarashi P. cDNA cloning and localization of a band 3-related protein from ileum. American Journal Of Physiology 1992, 263: g345-g352. PMID: 1415547, DOI: 10.1152/ajpgi.1992.263.3.g345.Peer-Reviewed Original ResearchConceptsRabbit ileal enterocytesDeduced amino acid sequenceBand 3Anion exchanger familyAmino acid sequenceAE gene familyBrush border membraneGene familyNonerythroid tissuesHCO3- exchangerErythroid band 3CDNA cloningPlasma membraneAcid sequenceExchanger familyRelated proteinsMembrane vesiclesIleal enterocytesMolecular identityCytoplasmic fragmentsProteinBasolateral membrane vesiclesBasolateral membraneMembraneEnterocytes
1991
cDNA cloning and immunolocalization of a Na(+)-H+ exchanger in LLC-PK1 renal epithelial cells
Reilly RF, Hildebrandt F, Biemesderfer D, Sardet C, Pouyssegur J, Aronson PS, Slayman CW, Igarashi P. cDNA cloning and immunolocalization of a Na(+)-H+ exchanger in LLC-PK1 renal epithelial cells. American Journal Of Physiology 1991, 261: f1088-f1094. PMID: 1661081, DOI: 10.1152/ajprenal.1991.261.6.f1088.Peer-Reviewed Original ResearchConceptsLLC-PK1 cellsFusion proteinAmino-terminal hydrophobic regionLLC-PK1 renal epithelial cellsHydrophobic regionAmino acid sequenceConfluent LLC-PK1 cellsRelative molecular massRenal epithelial cellsSignificant homologyHuman proteinsCDNA cloningAcid sequenceNucleotide identityHuman sequenceLibrary screeningCDNAMolecular massPolymerase chain reactionAmino acidsApical membraneProteinConfocal microscopyHydrophilic domainsLLC-PK1