2022
A volatile from the skin microbiota of flavivirus-infected hosts promotes mosquito attractiveness
Zhang H, Zhu Y, Liu Z, Peng Y, Peng W, Tong L, Wang J, Liu Q, Wang P, Cheng G. A volatile from the skin microbiota of flavivirus-infected hosts promotes mosquito attractiveness. Cell 2022, 185: 2510-2522.e16. PMID: 35777355, DOI: 10.1016/j.cell.2022.05.016.Peer-Reviewed Original ResearchSkin microbiotaMosquito-transmitted flavivirusDengue patientsFlavivirus infectionFlavivirus life cycleDietary administrationHealthy peopleCommensal bacteriaZika virusHost skinMosquito attractivenessArboviral transmissionRELMαAedes mosquitoesMicrobiotaMosquito olfactionInfected hostAntimicrobial proteinsHematophagous arthropodsHost-seeking activityMosquitoesIsotretinoinPatientsInfectionMice
2021
A human-blood-derived microRNA facilitates flavivirus infection in fed mosquitoes
Zhu Y, Zhang C, Zhang L, Yang Y, Yu X, Wang J, Liu Q, Wang P, Cheng G. A human-blood-derived microRNA facilitates flavivirus infection in fed mosquitoes. Cell Reports 2021, 37: 110091. PMID: 34910910, DOI: 10.1016/j.celrep.2021.110091.Peer-Reviewed Original Research
2020
Macrophage scavenger receptor 1 controls Chikungunya virus infection through autophagy in mice
Yang L, Geng T, Yang G, Ma J, Wang L, Ketkar H, Yang D, Lin T, Hwang J, Zhu S, Wang Y, Dai J, You F, Cheng G, Vella AT, Flavell RA, Fikrig E, Wang P. Macrophage scavenger receptor 1 controls Chikungunya virus infection through autophagy in mice. Communications Biology 2020, 3: 556. PMID: 33033362, PMCID: PMC7545163, DOI: 10.1038/s42003-020-01285-6.Peer-Reviewed Original ResearchConceptsMacrophage scavenger receptor 1Scavenger receptor 1Chikungunya virusReceptor 1Antiviral roleType I IFN responseChikungunya virus infectionLow-density lipoproteinImportant antiviral roleI IFN responseMarkers of autophagyCHIKV infectionViral loadArthritogenic alphavirusesVirus infectionCHIKV replicationATG5-ATG12Antiviral actionKnockout miceMSR1 expressionIFN responseInfectionMiceNsp1 proteinAutophagic functionA mosquito salivary protein promotes flavivirus transmission by activation of autophagy
Sun P, Nie K, Zhu Y, Liu Y, Wu P, Liu Z, Du S, Fan H, Chen CH, Zhang R, Wang P, Cheng G. A mosquito salivary protein promotes flavivirus transmission by activation of autophagy. Nature Communications 2020, 11: 260. PMID: 31937766, PMCID: PMC6959235, DOI: 10.1038/s41467-019-14115-z.Peer-Reviewed Original ResearchConceptsBeclin-1Viral transmissionFlavivirus transmissionMosquito salivary proteinsHost immune cellsZika virus transmissionActivation of autophagyLow viremiaProphylactic targetsMosquito salivaImmune cellsZIKV transmissionAllergen 1Infected mosquitoesViral infectionMonocyte lineageVirus transmissionMiceMosquitoesSalivary proteinsNumerous studiesViremiaInfectionFlavivirusesProtein
2019
The GRA15 protein from Toxoplasma gondii enhances host defense responses by activating the interferon stimulator STING
Wang P, Li S, Zhao Y, Zhang B, Li Y, Liu S, Du H, Cao L, Ou M, Ye X, Li P, Gao X, Wang P, Jing C, Shao F, Yang G, You F. The GRA15 protein from Toxoplasma gondii enhances host defense responses by activating the interferon stimulator STING. Journal Of Biological Chemistry 2019, 294: 16494-16508. PMID: 31416833, PMCID: PMC6851339, DOI: 10.1074/jbc.ra119.009172.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDisease Models, AnimalHEK293 CellsHumansImmunity, InnateInterferon-gammaInterleukin-12 Subunit p35Membrane ProteinsMiceMice, Inbred C57BLMice, KnockoutNucleotidyltransferasesProtein MultimerizationProtozoan ProteinsSpleenSurvival RateToxoplasmaToxoplasmosisTumor Necrosis Factor Receptor-Associated Peptides and ProteinsUbiquitinationConceptsImmune responseCyclic GMP-AMP synthaseWT miceRobust innate immune responseCGAS-deficient miceHost immune responseInnate immune responseType I IFNCGAS/STING signalingInterferon-stimulated genesGMP-AMP synthaseInflammatory cytokinesNeurotropic pathogensGRA15Mouse modelSevere symptomsI IFNLatent infectionSTING signalingHigh mortalityMiceInfectionHost defense responsesStingsHost cellsLack of efficacy of ivermectin for prevention of a lethal Zika virus infection in a murine system
Ketkar H, Yang L, Wormser GP, Wang P. Lack of efficacy of ivermectin for prevention of a lethal Zika virus infection in a murine system. Diagnostic Microbiology And Infectious Disease 2019, 95: 38-40. PMID: 31097261, PMCID: PMC6697611, DOI: 10.1016/j.diagmicrobio.2019.03.012.Peer-Reviewed Original ResearchConceptsZika virus infectionAnti-Zika virus activityVirus infectionAnimal modelsZika virusLethal Zika Virus InfectionIFNAR1 knockout miceZika virus strainLack of efficacyEffectiveness of ivermectinLethal infectionKnockout miceVirus activityAntiviral activityMurine systemVirus strainsDrug ivermectinInfectionIvermectinStudy limitationsPreventionVirusSenegal strainMiceAedes mosquitoes acquire and transmit Zika virus by breeding in contaminated aquatic environments
Du S, Liu Y, Liu J, Zhao J, Champagne C, Tong L, Zhang R, Zhang F, Qin CF, Ma P, Chen CH, Liang G, Liu Q, Shi PY, Cazelles B, Wang P, Tian H, Cheng G. Aedes mosquitoes acquire and transmit Zika virus by breeding in contaminated aquatic environments. Nature Communications 2019, 10: 1324. PMID: 30902991, PMCID: PMC6430813, DOI: 10.1038/s41467-019-09256-0.Peer-Reviewed Original Research
2018
UBXN3B positively regulates STING-mediated antiviral immune responses
Yang L, Wang L, Ketkar H, Ma J, Yang G, Cui S, Geng T, Mordue DG, Fujimoto T, Cheng G, You F, Lin R, Fikrig E, Wang P. UBXN3B positively regulates STING-mediated antiviral immune responses. Nature Communications 2018, 9: 2329. PMID: 29899553, PMCID: PMC5998066, DOI: 10.1038/s41467-018-04759-8.Peer-Reviewed Original ResearchConceptsUbiquitin regulatory X domain-containing proteinAntiviral immune responseImmune responseDeficient immune responseDomain-containing proteinsInterferon genes (STING) signalingVesicular stomatitis virus infectionDiverse biological processesStomatitis virus infectionPhosphorylation of TBK1Physiological evidenceHerpes simplex virus 1Cre-loxP approachSimplex virus 1Virus infectionAdult miceGene signalingHSV-1Biological processesPhysiological functionsVirus 1MicePrimary cellsConsequent recruitmentResponse
2016
Flavivirus NS1 protein in infected host sera enhances viral acquisition by mosquitoes
Liu J, Liu Y, Nie K, Du S, Qiu J, Pang X, Wang P, Cheng G. Flavivirus NS1 protein in infected host sera enhances viral acquisition by mosquitoes. Nature Microbiology 2016, 1: 16087. PMID: 27562253, PMCID: PMC5003325, DOI: 10.1038/nmicrobiol.2016.87.Peer-Reviewed Original ResearchConceptsNonstructural protein 1Japanese encephalitis virus nonstructural protein 1Dengue virusDENV nonstructural protein 1Flavivirus nonstructural protein 1Lethal DENV challengeNS1 proteinReceptor-deficient miceVirus nonstructural protein 1Flavivirus NS1 proteinAcquisition of virusDENV challengeActive immunizationFlaviviral diseasesImmune barrierHost serumViral transferInfected mammalian hostsViral acquisitionMosquito midgutMiceProtein 1Virus acquisitionImmunizationMosquito vectors
2011
prM-antibody renders immature West Nile virus infectious in vivo
Colpitts TM, Rodenhuis-Zybert I, Moesker B, Wang P, Fikrig E, Smit JM. prM-antibody renders immature West Nile virus infectious in vivo. Journal Of General Virology 2011, 92: 2281-2285. PMID: 21697345, PMCID: PMC3347797, DOI: 10.1099/vir.0.031427-0.Peer-Reviewed Original ResearchConceptsWest Nile virusInfectious West Nile virusNile virusDeath of micePrM antibodiesNeurotropic pathogensWNV particlesSevere human diseasesFamily FlaviviridaeVivo proofImmature flavivirus particlesInfectious potentialAntibodiesDiseaseViral surfaceVirus particlesPrM proteinFlavivirus particlesVirusHuman diseasesInfectionMiceFlavivirusesBrainSerum
2009
Antibodies against a Tick Protein, Salp15, Protect Mice from the Lyme Disease Agent
Dai J, Wang P, Adusumilli S, Booth CJ, Narasimhan S, Anguita J, Fikrig E. Antibodies against a Tick Protein, Salp15, Protect Mice from the Lyme Disease Agent. Cell Host & Microbe 2009, 6: 482-492. PMID: 19917502, PMCID: PMC2843562, DOI: 10.1016/j.chom.2009.10.006.Peer-Reviewed Original ResearchConceptsArthropod-borne pathogensTick-borne BorreliaTick salivary proteinsTick proteinsB. burgdorferiLyme diseaseDisease agentsTick-borne illnessB. burgdorferi infectionLyme disease agentHuman vaccinesSalp15Infection of miceB. burgdorferi antigensMicrobial toxinsMammalian hostsBorrelia burgdorferiPathogensMechanism of actionBurgdorferi infectionProtect miceMedical importanceBurgdorferiProtective capacityMiceThe Urokinase Receptor (uPAR) Facilitates Clearance of Borrelia burgdorferi
Hovius JW, Bijlsma MF, van der Windt GJ, Wiersinga WJ, Boukens BJ, Coumou J, Oei A, de Beer R, de Vos AF, van 't Veer C, van Dam AP, Wang P, Fikrig E, Levi MM, Roelofs JJ, van der Poll T. The Urokinase Receptor (uPAR) Facilitates Clearance of Borrelia burgdorferi. PLOS Pathogens 2009, 5: e1000447. PMID: 19461880, PMCID: PMC2678258, DOI: 10.1371/journal.ppat.1000447.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArthritis, InfectiousBorrelia burgdorferiCell MovementHeartHistocytochemistryHumansLeukocytesLyme DiseaseMiceMice, Inbred C57BLMice, KnockoutMyocarditisPhagocytosisReceptors, Urokinase Plasminogen ActivatorSkinStatistics, NonparametricUp-RegulationUrinary BladderUrokinase-Type Plasminogen ActivatorConceptsB. burgdorferi numbersWT controlsPhagocytotic capacityC3H/HeN backgroundIL-1beta mRNA expressionBorrelia burgdorferiB. burgdorferi infectionRole of uPARSevere carditisBurgdorferi infectionImmune responseLeukocyte functionSpirochete Borrelia burgdorferiFibrinolytic systemPAI-1Facilitate clearanceMRNA expressionHuman leukocytesLyme borreliosisMiceB. burgdorferiCausative agentProteinase receptorUPARAdequate eradication
2008
ICAM-1 Participates in the Entry of West Nile Virus into the Central Nervous System
Dai J, Wang P, Bai F, Town T, Fikrig E. ICAM-1 Participates in the Entry of West Nile Virus into the Central Nervous System. Journal Of Virology 2008, 82: 4164-4168. PMID: 18256150, PMCID: PMC2292986, DOI: 10.1128/jvi.02621-07.Peer-Reviewed Original ResearchConceptsWest Nile virusICAM-1Control animalsWest Nile virus neuroinvasionBlood-brain barrier leakagePathogenesis of encephalitisNile virusBlood-brain barrierLow viral loadWest Nile encephalitisCentral nervous systemICAM-1 participatesVirus neuroinvasionNeuronal damageLeukocyte infiltrationViral encephalitisViral loadBarrier leakageViral infectionNervous systemEncephalitisMiceICAMVirusAnimals
2007
Borrelia burgdorferi basic membrane proteins A and B participate in the genesis of Lyme arthritis
Pal U, Wang P, Bao F, Yang X, Samanta S, Schoen R, Wormser GP, Schwartz I, Fikrig E. Borrelia burgdorferi basic membrane proteins A and B participate in the genesis of Lyme arthritis. Journal Of Experimental Medicine 2007, 205: 133-141. PMID: 18166585, PMCID: PMC2234379, DOI: 10.1084/jem.20070962.Peer-Reviewed Original ResearchConceptsLyme arthritisMouse jointsB. burgdorferi antigensBurgdorferi-infected miceSevere arthritisSpirochete numbersArthritisHost responseLyme diseaseAffinity-purified antibodiesBorrelia burgdorferiChain reactionMiceOriginal phenotypeBasic membrane proteinMutant spirochetesGene expressionJointsInflammationPathogenesisAntigenDiseaseB. burgdorferi gene expression