2005
Orphan Nuclear Receptor GCNF Is Required for the Repression of Pluripotency Genes during Retinoic Acid-Induced Embryonic Stem Cell Differentiation
Gu P, LeMenuet D, Chung A, Mancini M, Wheeler DA, Cooney AJ. Orphan Nuclear Receptor GCNF Is Required for the Repression of Pluripotency Genes during Retinoic Acid-Induced Embryonic Stem Cell Differentiation. Molecular And Cellular Biology 2005, 25: 8507-8519. PMID: 16166633, PMCID: PMC1265758, DOI: 10.1128/mcb.25.19.8507-8519.2005.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, NorthernBlotting, WesternCell DifferentiationCell LineCell NucleusChromatin ImmunoprecipitationDNA-Binding ProteinsDown-RegulationEmbryo, MammalianFemaleFibroblast Growth Factor 4GenotypeHomeodomain ProteinsIn Situ HybridizationMaleMiceMice, TransgenicMicroscopy, FluorescenceModels, GeneticNanog Homeobox ProteinNuclear Receptor Subfamily 6, Group A, Member 1Octamer Transcription Factor-3PhenotypePlasmidsProtein BindingReceptors, Cytoplasmic and NuclearResponse ElementsReverse Transcriptase Polymerase Chain ReactionSignal TransductionSOXB1 Transcription FactorsStem CellsTime FactorsTrans-ActivatorsTransfectionTretinoinConceptsLoss of repressionES cell differentiationPluripotency genesCell differentiationTranscription factorsEmbryonic developmentES cellsEmbryonic stem cell pluripotencyEmbryonic stem cell differentiationEarly mouse embryonic developmentStem cell pluripotencyMouse embryonic developmentPluripotency gene expressionEarly embryonic developmentInitiation of differentiationStem cell differentiationRetinoic acidCell pluripotencyNanog geneGenes Oct4Somatic cellsUndifferentiated stateGene expressionGCNFRepression
2003
GCNF‐dependent repression of BMP‐15 and GDF‐9 mediates gamete regulation of female fertility
Lan Z, Gu P, Xu X, Jackson KJ, DeMayo FJ, O'Malley BW, Cooney AJ. GCNF‐dependent repression of BMP‐15 and GDF‐9 mediates gamete regulation of female fertility. The EMBO Journal 2003, 22: 4070-4081. PMID: 12912906, PMCID: PMC175795, DOI: 10.1093/emboj/cdg405.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone Morphogenetic Protein 15Bone Morphogenetic ProteinsCHO CellsCricetinaeDNA-Binding ProteinsEgg ProteinsFemaleFertilityGene Expression RegulationGrowth Differentiation Factor 9IntegrasesIntercellular Signaling Peptides and ProteinsMembrane GlycoproteinsMiceMice, KnockoutMice, TransgenicModels, BiologicalNuclear Receptor Subfamily 6, Group A, Member 1OocytesOvaryReceptors, Cell SurfaceReceptors, Cytoplasmic and NuclearRepressor ProteinsTransforming Growth Factor betaTransgenesViral ProteinsZona PellucidaZona Pellucida GlycoproteinsConceptsGerm cell nuclear factorBMP-15Bone morphogenetic protein 15New regulatory pathwayFemale fertilityGDF-9Growth differentiation factor 9DR0 elementsDifferentiation factor 9Knockout mouse modelSomatic cellsGene promoterRegulatory pathwaysReproductive defectsReporter activityFemale reproductionMolecular studiesFactor 9Paracrine communicationProtein 15Nuclear factorAberrant steroidogenesisExpressionOocytesPrimary defect