2005
Identification of Binding Sites of EVI1 in Mammalian Cells*
Yatsula B, Lin S, Read AJ, Poholek A, Yates K, Yue D, Hui P, Perkins AS. Identification of Binding Sites of EVI1 in Mammalian Cells*. Journal Of Biological Chemistry 2005, 280: 30712-30722. PMID: 16006653, DOI: 10.1074/jbc.m504293200.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceBinding SitesDNADNA-Binding ProteinsHerpes Simplex Virus Protein Vmw65HumansMDS1 and EVI1 Complex Locus ProteinMiceMolecular Sequence DataMutagenesis, Site-DirectedMutation, MissenseNIH 3T3 CellsOligonucleotide Array Sequence AnalysisProtein ConformationProto-OncogenesRecombinant Fusion ProteinsTranscription FactorsZinc FingersConceptsChromatin immunoprecipitationTarget genesN-terminal DNAPutative target genesVP16 fusion proteinTranscription start siteN-terminal domainGel shift assaysNIH 3T3 cellsZFPM2/FOG2Transcriptional activatorEndogenous genesMissense mutantsEVI1 bindsZinc fingerMammalian cellsStart siteShift assaysMutant formsFusion proteinTransactivation studiesSequence analysisGenesEVI1Binding sites
2002
Assessment of RET/PTC Oncogene Activation and Clonality in Thyroid Nodules with Incomplete Morphological Evidence of Papillary Carcinoma A Search for the Early Precursors of Papillary Cancer
Fusco A, Chiappetta G, Hui P, Garcia-Rostan G, Golden L, Kinder BK, Dillon DA, Giuliano A, Cirafici AM, Santoro M, Rosai J, Tallini G. Assessment of RET/PTC Oncogene Activation and Clonality in Thyroid Nodules with Incomplete Morphological Evidence of Papillary Carcinoma A Search for the Early Precursors of Papillary Cancer. American Journal Of Pathology 2002, 160: 2157-2167. PMID: 12057919, PMCID: PMC1850819, DOI: 10.1016/s0002-9440(10)61164-9.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAnimalsBase SequenceCarcinoma, PapillaryCloning, MolecularDrosophila ProteinsGene Expression Regulation, NeoplasticGenetic MarkersHumansMembrane ProteinsMiceMolecular Sequence DataNeoplasm InvasivenessNuclear Receptor CoactivatorsOncogene ProteinsOncogene Proteins, FusionPatched ReceptorsPatched-1 ReceptorProtein-Tyrosine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-retRabbitsReceptor Protein-Tyrosine KinasesReceptors, Cell SurfaceReverse Transcriptase Polymerase Chain ReactionThyroid NeoplasmsThyroid NoduleTranscription FactorsConceptsLaser capture microdissectionPapillary cancerRET/PTC1RET immunoreactivityThyroid nodulesCarcinoma featuresMicroscopic fociPapillary carcinomaInvasive papillary cancerCytological alterationsReverse transcriptase-polymerase chain reactionRET activationRET/PTC3 rearrangementsTranscriptase-polymerase chain reactionRET/PTC3Analysis of clonalityRET/PTC oncogene activationPrecursor lesionsEntire lesionTumor fociPolyclonal tumorsAdenomatous nodulesMorphological changesSame tumorMorphological signs
1998
Identification of candidate target genes for EVI-1, a zinc finger oncoprotein, using a novel selection strategy
Kim J, Hui P, Yue D, Aycock J, Leclerc C, Bjoring A, Perkins A. Identification of candidate target genes for EVI-1, a zinc finger oncoprotein, using a novel selection strategy. Oncogene 1998, 17: 1527-1538. PMID: 9794230, DOI: 10.1038/sj.onc.1202331.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAnimalsBase SequenceBinding SitesCalcium ChannelsDNA, ComplementaryDNA-Binding ProteinsEscherichia coliExonsGenomic LibraryHaploidyHumansInositol 1,4,5-Trisphosphate ReceptorsMDS1 and EVI1 Complex Locus ProteinMiceMolecular Sequence DataOncogene ProteinsProto-OncogenesReceptors, Cytoplasmic and NuclearRecombinant Fusion ProteinsSequence AlignmentTranscription FactorsTumor Cells, CulturedZinc FingersConceptsGenomic fragmentEvi-1Target genesZinc finger proteinHybrid selectionCandidate target genesTetracycline-regulated systemChimeric activatorFinger proteinHaploid genomeMouse cDNAMouse genomeGene sequencesMouse DNAFusion proteinTwo-step selectionGenesGenomeProteinCDNANovel selection strategyFragmentsHigh affinitySublibrariesITPR2