2024
Abstract 1147: Crosstalk Between Alk5 And Mtorc1 Signaling Promotes VSMC Differentiation And The Therapeutic Effect Of Rapamycin
Chakraborty R, Chatterjee P, Dave J, Obrien B, Joshi D, Schulz V, Greif D, Hwa J, Gallagher P, Martin K. Abstract 1147: Crosstalk Between Alk5 And Mtorc1 Signaling Promotes VSMC Differentiation And The Therapeutic Effect Of Rapamycin. Arteriosclerosis Thrombosis And Vascular Biology 2024, 44 DOI: 10.1161/atvb.44.suppl_1.1147.Peer-Reviewed Original ResearchVascular smooth muscle cellsTherapeutic effect of rapamycinEffects of rapamycinVSMC differentiationContractile genesConsistent with in vitro findingsRapamycin treatmentCarotid artery injuryHuman coronary artery SMCsVascular smooth muscle cell differentiationIntimal hyperplasiaSmooth muscle cellsCoronary artery SMCsMTORC1 inhibitor rapamycinPhosphorylation of Smad2/3Inhibition of ALK5Smad-binding elementSmad transcription factorsALK5 activityArterial injuryArtery SMCsKnockout miceInhibition of mTORC1Vascular smooth muscle cell plasticityMuscle cellsSurvey research in perinatal medicine
Conroy S, Gallagher P. Survey research in perinatal medicine. Journal Of Perinatology 2024, 44: 771-772. PMID: 38538912, DOI: 10.1038/s41372-024-01940-4.Peer-Reviewed Original ResearchThe diagnostic utility of obtaining two blood cultures for the diagnosis of early onset sepsis in neonates
Fleiss N, Shabanova V, Murray T, Gallagher P, Bizzarro M. The diagnostic utility of obtaining two blood cultures for the diagnosis of early onset sepsis in neonates. Journal Of Perinatology 2024, 44: 745-747. PMID: 38409330, DOI: 10.1038/s41372-024-01914-6.Peer-Reviewed Original ResearchDiagnosis and management of pyruvate kinase deficiency: international expert guidelines
Al-Samkari H, Shehata N, Lang-Robertson K, Bianchi P, Glenthøj A, Sheth S, Neufeld E, Rees D, Chonat S, Kuo K, Rothman J, Barcellini W, van Beers E, Pospíšilová D, Shah A, van Wijk R, Glader B, Mañú Pereira M, Andres O, Kalfa T, Eber S, Gallagher P, Kwiatkowski J, Galacteros F, Lander C, Watson A, Elbard R, Peereboom D, Grace R. Diagnosis and management of pyruvate kinase deficiency: international expert guidelines. The Lancet Haematology 2024, 11: e228-e239. PMID: 38330977, DOI: 10.1016/s2352-3026(23)00377-0.Peer-Reviewed Original ResearchExpert panelCountry of practiceCare of patientsEvidence-based guidelinesQuality of lifeClinical care of patientsPyruvate kinase deficiencySystematic literature searchDeficient careGuideline methodologistsPK deficiencyClinical careClinical guidelinesInternational expert guidelinesEstimated prevalenceHaemolytic anaemiaConsensus procedureManagement of chronic complicationsDraft recommendationsGRADE methodologyAGREE II frameworkExpert guidelinesLiterature searchClinical practiceInternational guidelinesJoint bleeds in mild hemophilia: Prevalence and clinical characteristics
Chiari J, Prozora S, Feinn R, Louizos E, Gallagher P, Bona R. Joint bleeds in mild hemophilia: Prevalence and clinical characteristics. Haemophilia 2024, 30: 331-335. PMID: 38240020, DOI: 10.1111/hae.14939.Peer-Reviewed Original ResearchMild haemophiliaJoint bleedsJoint range of motionHepatitis CRange of motionMild diseaseHistory of joint bleedsInstitutional review board approvalJoint rangeRetrospective chart reviewHemophilia treatment centersReview board approvalJoint healthHistory of traumaDisease burdenMedical recordsChart reviewClinical characteristicsMitigate disease burdenRisk factorsBoard approvalBleedingTraumatic bleedingAverage ageHemophilic arthropathy
2023
Multimodality Platelet Evaluation By Mass Cytometry and Genetic Analysis in Patients with Bleeding Disorders
Gu S, Gallagher P, Butt A, Gu V, Lezon-Geyda K, Schulz V, Prozora S, Lee A, Neparidze N, Bar N, Martin K, Cornell J, Chirico G, Chakraborty R, Rinder H, Hwa J, Bona R. Multimodality Platelet Evaluation By Mass Cytometry and Genetic Analysis in Patients with Bleeding Disorders. Blood 2023, 142: 1197. DOI: 10.1182/blood-2023-177946.Peer-Reviewed Original ResearchBleeding tendencyBleeding disorderPlatelet markersPlatelet aggregometryMass cytometryPlatelet functionPlatelet disordersSingle-center prospective studyLow-risk groupAbnormal bleeding tendencyQualitative platelet disordersLarge patient cohortQuantitative platelet disordersCommon underlying causeGenetic variantsMultimodality evaluationTotal patientsRisk stratificationLaboratory suspicionPlatelet dysfunctionProspective studyPatient cohortUnivariate analysisPlatelet volumeRisk groupsIdentification of a Novel Gene Regulatory Element in Human Erythroid Progenitor Cells
Schulz V, Lezon-Geyda K, Shan P, Papoin J, Narla M, Steiner L, Blanc L, Palis J, Gallagher P. Identification of a Novel Gene Regulatory Element in Human Erythroid Progenitor Cells. Blood 2023, 142: 9. DOI: 10.1182/blood-2023-186046.Peer-Reviewed Original ResearchErythroid progenitor cellsActive enhancersEarly erythropoiesisChIP-seqRegulatory elementsGene expressionATAC-seqRUNX motifsGATA motifSingle nucleotide polymorphismsProgenitor cellsGATA1 bindingDisease genesEnhancer regionErythroid lineageTissue-specific transcription factorsNovel gene regulatory elementsNovel enhancer regionErythrocyte traitsGenome-wide association studiesDirect tissue-specific expressionNovel regulatory elementGene regulatory elementsHuman erythroid progenitor cellsTerminal erythroid differentiationPhenotypic and Proteomic Characterization of the Human Erythroid Progenitor Continuum Reveal Dynamic Changes in Cell Cycle and in Metabolic Pathways
Papoin J, Yan H, Leduc M, le-Gall M, Narla A, Steiner L, Gallagher P, Hillyer C, Gauthier E, Narla M, Blanc L. Phenotypic and Proteomic Characterization of the Human Erythroid Progenitor Continuum Reveal Dynamic Changes in Cell Cycle and in Metabolic Pathways. Blood 2023, 142: 2455. DOI: 10.1182/blood-2023-189566.Peer-Reviewed Original ResearchCell cycleFaster cycling cellsProgenitor differentiationProgenitor populationsOxidative phosphorylationMass spectrometry-based proteomicsErythroid progenitorsS phaseProtein copy numbersSpectrometry-based proteomicsErythroid progenitor differentiationCycle-related genesG2/MTerminal erythropoiesisProgenitor biologyProteomic characterizationHematopoietic stem cellsE2F membersHuman erythropoiesisHuman bone marrowFunctional analysisErythroid lineageMurine erythropoiesisMetabolic pathwaysS transitionErythroid Progenitor Cells in the Murine Bone Marrow: Parallels with Human Counterparts and Response to Acute Anemia
McGrath K, Kingsley P, Rust E, Schulz V, Koniski A, Schofield T, Vit L, Narla M, Blanc L, Steiner L, Gallagher P, Palis J. Erythroid Progenitor Cells in the Murine Bone Marrow: Parallels with Human Counterparts and Response to Acute Anemia. Blood 2023, 142: 2451. DOI: 10.1182/blood-2023-187741.Peer-Reviewed Original ResearchGene Ontology termsErythroid progenitor cellsErythroid progenitorsOntology termsTranscription factorsProgenitor cellsMurine erythroid progenitor cellsEPO-responsive genesMurine counterpartGlobal transcriptomic studiesLineage-specific progenitor cellsHuman erythroid progenitor cellsAnalysis of genesCholesterol homeostasisHuman counterpartLate-stage erythroid progenitorsMature red blood cellsUpregulated transcription factorsMurine erythroid progenitorsRNA-seq studiesCholesterol biosynthesis genesColony-forming progenitorsBiosynthesis genesErythroid progenitor populationsMurine bone marrowRps19 and Rpl5 Play Distinct Roles in hematopoietic Stem Cell maintenance and Erythroid Differentiation
Tang Y, Ling T, Khan M, Rao R, Schulz V, Papoin J, Narla A, Lipton J, Palis J, Steiner L, Gallagher P, Narla M, Crispino J, Blanc L. Rps19 and Rpl5 Play Distinct Roles in hematopoietic Stem Cell maintenance and Erythroid Differentiation. Blood 2023, 142: 144. DOI: 10.1182/blood-2023-189146.Peer-Reviewed Original ResearchFailure of erythropoiesisErythroid differentiationVav-iCreHematopoietic stem cell maintenanceFetal hematopoiesisHematopoietic stemProtein translation ratesStem cell maintenanceRibosomal protein haploinsufficiencyGlobal protein synthesisTerminal erythroid differentiationCell compartmentExpression of RUNX1Stem cell compartmentErythroid fateProgenitor cell compartmentFetal liverRibosome biogenesisPolysome profilingNormal expression levelsRibosomal proteinsProgenitor biologyCell maintenanceScRNAseq studiesTranscription factorsA Novel β-Globin Locus Deletional Syndrome: εγ-Thalassemia
Gallagher P. A Novel β-Globin Locus Deletional Syndrome: εγ-Thalassemia. Clinical Chemistry 2023, 69: 671-672. PMID: 37279577, DOI: 10.1093/clinchem/hvad067.Peer-Reviewed Original Research
2022
Developmental Stage-Specific Hematopoietic Failure In Vivo in a Novel Model of Diamond Blackfan Anemia
Tang Y, Kahn M, Papoin J, Yan H, Narla A, Palis J, Steiner L, Gallagher P, Lipton J, Narla M, Blanc L. Developmental Stage-Specific Hematopoietic Failure In Vivo in a Novel Model of Diamond Blackfan Anemia. Blood 2022, 140: 2946. DOI: 10.1182/blood-2022-163200.Peer-Reviewed Original ResearchBMI1 Regulates Proliferation of Human Late-Stage Erythroid Progenitors
Olsen J, McGrath K, Murphy K, Schofield T, Getman M, Narla M, Blanc L, Schulz V, Gallagher P, Steiner L, Palis J. BMI1 Regulates Proliferation of Human Late-Stage Erythroid Progenitors. Blood 2022, 140: 8152. DOI: 10.1182/blood-2022-167830.Peer-Reviewed Original ResearchGender analysis of Journal of Perinatology authorship during COVID-19
Gadek L, Dammann C, Savich R, Mmuo-Oji C, Barrera L, Gallagher P, Machut K. Gender analysis of Journal of Perinatology authorship during COVID-19. Journal Of Perinatology 2022, 43: 518-522. PMID: 36335276, PMCID: PMC9638437, DOI: 10.1038/s41372-022-01551-x.Peer-Reviewed Original ResearchAnemia in the pediatric patient
Gallagher PG. Anemia in the pediatric patient. Blood 2022, 140: 571-593. PMID: 35213686, PMCID: PMC9373018, DOI: 10.1182/blood.2020006479.Peer-Reviewed Original ResearchConceptsLow birth weightIron deficiency anemiaNovel pharmacologic agentsWorld Health OrganizationNeurologic complicationsHeart failurePediatric patientsPhysical examinationBirth weightDeficiency anemiaChronic diseasesNutritional anemiaPharmacologic agentsSyndromic causesUndiagnosed casesTreatment strategiesCommon causeFamily historyPopulation-based approachAnemiaImportant causePreschool-age childrenDiagnostic testingGenetic testingDysmorphic featuresExtramedullary hematopoietic stem cells
Gallagher P. Extramedullary hematopoietic stem cells. Blood 2022, 139: 3353-3354. PMID: 35679074, DOI: 10.1182/blood.2022015879.Peer-Reviewed Original ResearchHMGB1-mediated restriction of EPO signaling contributes to anemia of inflammation
Dulmovits BM, Tang Y, Papoin J, He M, Li J, Yang H, Addorisio ME, Kennedy L, Khan M, Brindley E, Ashley RJ, Ackert-Bicknell C, Hale J, Kurita R, Nakamura Y, Diamond B, Barnes BJ, Hermine O, Gallagher PG, Steiner LA, Lipton JM, Taylor N, Mohandas N, Andersson U, Al-Abed Y, Tracey KJ, Blanc L. HMGB1-mediated restriction of EPO signaling contributes to anemia of inflammation. Blood 2022, 139: 3181-3193. PMID: 35040907, PMCID: PMC9136881, DOI: 10.1182/blood.2021012048.Peer-Reviewed Original ResearchConceptsAnemia of inflammationDamage-associated molecular pattern moleculesHigh-mobility group box 1 proteinMobility group box 1 proteinErythroid precursorsGroup box 1 proteinAdvanced glycation end productsAnti-HMGB1 antibodyGlycation end productsMolecular pattern moleculesChronic phaseSepsis onsetChronic diseasesHMGB1 receptorsAnemia developmentPattern moleculesAnemiaGenetic ablationInflammationMurine precursorRefractory stateHMGB1Reduced expansionEPO signalingDeleterious effectsHistone Acetyltransferases p300 and CBP Coordinate Distinct Chromatin Remodeling Programs in Vascular Smooth Muscle Plasticity
Chakraborty R, Ostriker AC, Xie Y, Dave JM, Gamez-Mendez A, Chatterjee P, Abu Y, Valentine J, Lezon-Geyda K, Greif DM, Schulz VP, Gallagher PG, Sessa WC, Hwa J, Martin KA. Histone Acetyltransferases p300 and CBP Coordinate Distinct Chromatin Remodeling Programs in Vascular Smooth Muscle Plasticity. Circulation 2022, 145: 1720-1737. PMID: 35502657, DOI: 10.1161/circulationaha.121.057599.Peer-Reviewed Original ResearchConceptsHistone acetylationContractile genesContractile protein expressionPhenotypic switchingHistone acetyl transferase p300Human intimal hyperplasiaPlatelet-derived growth factor treatmentAcetyl transferase p300Key regulatory mechanismSmooth muscle cell phenotypeP300 expressionP300-dependent acetylationSmooth muscle plasticityDistinct functional interactionsMuscle cell phenotypeProtein expressionIntimal hyperplasiaRole of p300Methylcytosine dioxygenase TET2Chromatin modificationsEpigenetic regulationVSMC phenotypic switchingSpecific histoneCardiovascular diseaseMaster regulator
2021
The Journal of Perinatology: looking forward
Gallagher PG. The Journal of Perinatology: looking forward. Journal Of Perinatology 2021, 41: 2700-2701. PMID: 34907365, PMCID: PMC8669232, DOI: 10.1038/s41372-021-01291-4.Peer-Reviewed Original ResearchSummary of Joint European Hematology Association (EHA) and EuroBloodNet Recommendations on Diagnosis and Treatment of Methemoglobinemia
Iolascon A, Andolfo I, Russo R, Barcellini W, Fermo E, Toldi G, Ghirardello S, Rees D, Van Wijk R, Kattamis A, Gallagher PG, Roy N, Taher A, Mohty R, Kulozik A, De Franceschi L, Gambale A, De Montalembert M, Forni GL, Harteveld CL, Prchal J, Bianchi P, Cell and Iron of EHA and EuroBloodNet S. Summary of Joint European Hematology Association (EHA) and EuroBloodNet Recommendations on Diagnosis and Treatment of Methemoglobinemia. HemaSphere 2021, 5: e660. PMID: 34805766, PMCID: PMC8598222, DOI: 10.1097/hs9.0000000000000660.Peer-Reviewed Original Research