2018
MRTFA augments megakaryocyte maturation by enhancing the SRF regulatory axis
Rahman NT, Schulz VP, Wang L, Gallagher PG, Denisenko O, Gualdrini F, Esnault C, Krause DS. MRTFA augments megakaryocyte maturation by enhancing the SRF regulatory axis. Blood Advances 2018, 2: 2691-2703. PMID: 30337297, PMCID: PMC6199649, DOI: 10.1182/bloodadvances.2018019448.Peer-Reviewed Original ResearchConceptsSerum response factorHEL cellsTarget genesBinding of SRFMegakaryocyte maturationActivity of SRFSRF target genesUpregulated target genesMyocardin family proteinsTernary complex factor familyTransformation-specific proteinsPrimary hematopoietic cellsHuman erythroleukemia cell lineErythroleukemia cell lineCArG sitesPrimary human CD34Genomic sitesGenomic regionsChromatin immunoprecipitationETS factorsTranscription factorsHuman megakaryopoiesisGenomic associationsMRTFAFactor family
2016
Adult human megakaryocyte-erythroid progenitors are in the CD34+CD38mid fraction
Sanada C, Xavier-Ferrucio J, Lu YC, Min E, Zhang PX, Zou S, Kang E, Zhang M, Zerafati G, Gallagher PG, Krause DS. Adult human megakaryocyte-erythroid progenitors are in the CD34+CD38mid fraction. Blood 2016, 128: 923-933. PMID: 27268089, PMCID: PMC4990855, DOI: 10.1182/blood-2016-01-693705.Peer-Reviewed Original ResearchConceptsMegakaryocyte/erythroid progenitorsComparative expression analysisNovel enrichment strategyMegakaryocyte-erythroid progenitorsPurification strategySingle-cell levelShort hairpin RNAFate decisionsE lineageNovel purification strategyLineage fateLineage commitmentGranulocyte colony-stimulating factor-mobilized peripheral bloodMK lineageExpression analysisE progenitorsErythroid lineageFactor-mobilized peripheral bloodDifferential expressionES cellsErythroid progenitorsMYB knockdownHairpin RNALineagesColony-forming units
2012
MKL1 and MKL2 play redundant and crucial roles in megakaryocyte maturation and platelet formation
Smith EC, Thon JN, Devine MT, Lin S, Schulz VP, Guo Y, Massaro SA, Halene S, Gallagher P, Italiano JE, Krause DS. MKL1 and MKL2 play redundant and crucial roles in megakaryocyte maturation and platelet formation. Blood 2012, 120: 2317-2329. PMID: 22806889, PMCID: PMC3447785, DOI: 10.1182/blood-2012-04-420828.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine DiphosphateAnimalsBleeding TimeBlood PlateletsBone Marrow CellsCells, CulturedCrosses, GeneticCytoplasmCytoskeletonGene Expression ProfilingHematopoiesisMegakaryocytesMiceMice, Inbred C57BLMice, KnockoutOligonucleotide Array Sequence AnalysisPlatelet ActivationThrombocytopeniaTrans-ActivatorsTranscription FactorsConceptsMegakaryocyte maturationPlatelet formationSerum response factorSerum response factor expressionMembrane organizationGene expressionMKL1MKL2Response factorDKO miceKO backgroundMegakaryocyte compartmentMegakaryocytesCritical roleMegakaryocyte ploidyExpressionMaturationKnockout miceFactor expressionCrucial roleHomologuesGenesMiceProlonged bleeding timeRole
2007
Novel role for EKLF in megakaryocyte lineage commitment
Frontelo P, Manwani D, Galdass M, Karsunky H, Lohmann F, Gallagher PG, Bieker JJ. Novel role for EKLF in megakaryocyte lineage commitment. Blood 2007, 110: 3871-3880. PMID: 17715392, PMCID: PMC2190608, DOI: 10.1182/blood-2007-03-082065.Peer-Reviewed Original ResearchConceptsErythroid gene regulationKrüppel-like factorMegakaryocyte-erythroid progenitorsFormation of megakaryocytesGene regulationTranscriptional regulatorsLineage commitmentTranscription factorsHematopoietic differentiationErythroid differentiationCommon progenitorExpression profilingErythroid cellsEKLFMegakaryocyte lineageNovel roleMolecular analysisLineagesMessage levelsFunction studiesMegakaryocytesProgenitorsDifferentiationRepressionGenes