2009
Patterns of Monomethylation of Histone H3 Lysine 27 Influence Gene Expression in a Cell-Type Specific Manner.
Steiner L, Schulz V, Maksimova Y, Wong C, Tuck D, Gallagher P. Patterns of Monomethylation of Histone H3 Lysine 27 Influence Gene Expression in a Cell-Type Specific Manner. Blood 2009, 114: 4585. DOI: 10.1182/blood.v114.22.4585.4585.Peer-Reviewed Original ResearchTranscription start siteNon-erythroid cellsPost-translational histone modificationsHistone H3 lysine 27Cell type-specific mannerH3 lysine 27Gene expressionGene repressionHistone modificationsActive transcriptionLysine 27Start siteHistone H3 lysine 4Expression arraysHistone H3 lysine 9Beta-globin locusH3 lysine 4Regions of heterochromatinH3 lysine 9Influence gene expressionMRNA transcript analysisType-specific mannerCell-type specificGene expression variesChromatin architecture
2008
Local and Distant Elements Regulate Tissue-Specific Expression of ANK-1 Gene Transcripts
Owen A, Laflamme K, Pilon A, Garrett L, Gallagher P, Bodine D. Local and Distant Elements Regulate Tissue-Specific Expression of ANK-1 Gene Transcripts. Blood 2008, 112: 2432. DOI: 10.1182/blood.v112.11.2432.2432.Peer-Reviewed Original ResearchNon-erythroid cellsHypersensitive sitesTransient transfection assaysConsensus sequenceChromatin loopsGATA-1NF-E2Promoter sequencesKb regionHistone acetylationErythroid transcription factor NF-E2Transfection assaysFirst exonDNase IErythroid cellsDevelopmental stage-specific patternsLocal chromatin architectureProtein-coding genesChromatin conformation captureANK-1 geneRNA Pol IITranscription initiation complexErythroid K562 cellsDNase hypersensitive sitesGATA-1 binding
2005
Chromatin Conformation and a Distal Regulatory Element Activate the Human Erythroid Ankyrin-1 Promoter.
Owen A, Liem R, Pilon A, Gallagher P, Bodine D. Chromatin Conformation and a Distal Regulatory Element Activate the Human Erythroid Ankyrin-1 Promoter. Blood 2005, 106: 803. DOI: 10.1182/blood.v106.11.803.803.Peer-Reviewed Original ResearchNon-erythroid cellsRegulatory elementsReporter geneErythroid cellsKb regionLuciferase reporter geneDNase IKb downstreamKb upstreamK562 cellsErythroid-specific transcription factorDNase I hypersensitive sitesE-box binding proteinGreen fluorescent protein (GFP) reporter geneCell typesChromatin conformation captureFluorescent protein reporter geneGFP reporter genePositive regulatory elementErythrocyte membrane skeletonConformation captureChromatin conformationChromatin loopsChromatin structureTranscription factors