2007
Multiple Defects of Both Primitive and Definitive Erythrocytes in EKLF-Deficient Mice.
Pilon A, Beaupre J, Bieker J, Gallagher P, Bodine D. Multiple Defects of Both Primitive and Definitive Erythrocytes in EKLF-Deficient Mice. Blood 2007, 110: 1234. DOI: 10.1182/blood.v110.11.1234.1234.Peer-Reviewed Original ResearchIngenuity Pathway AnalysisFL cellsSevere anemiaFetal liver cellsG0/G1Cell cycle analysisColony-forming assaysLevels of mRNADay 14Cytometric analysisAbsolute numberLiver cellsCell cycle progressionComparable reductionMiceDifferentiation blockOrthochromatic normoblastsNormoblastsKrüppel-like factorAnemiaTranscription factorsApoptosisOsmotic fragility assaysPathway analysisWT levels
2005
Genetic Evidence That an 11 Amino Acid β-Hairpin Loop in the Cytoplasmic Domain of Band 3 Is Responsible for at Least 50% of Ankyrin Binding in Mouse Erythrocytes.
Markham N, Parry E, Stefanovic M, Gallagher P, Low P, Bodine D. Genetic Evidence That an 11 Amino Acid β-Hairpin Loop in the Cytoplasmic Domain of Band 3 Is Responsible for at Least 50% of Ankyrin Binding in Mouse Erythrocytes. Blood 2005, 106: 1659. DOI: 10.1182/blood.v106.11.1659.1659.Peer-Reviewed Original ResearchΒ-hairpin loopCytoplasmic domainWild typeHuman band 3Neomycin resistance geneMembrane proteinsBand 3Mutant allelesBand 3 cytoplasmic domainResistance genesLevels of mRNARed cell membrane proteinsIntegral erythrocyte membrane proteinsSpectrin-actin cytoskeletonCre recombinase proteinDomain 3Embryonic stem cellsQuantitative RT-PCR analysisCell membrane proteinsNormal Mendelian ratioBand 3 mRNATransgenic miceAnion exchange proteinMutant erythrocytesBand 3 gene