2008
Ankyrin‐linked hereditary spherocytosis in an African–American kindred
Sangerman J, Maksimova Y, Edelman EJ, Morrow JS, Forget BG, Gallagher PG. Ankyrin‐linked hereditary spherocytosis in an African–American kindred. American Journal Of Hematology 2008, 83: 789-794. PMID: 18704959, PMCID: PMC11304496, DOI: 10.1002/ajh.21254.Peer-Reviewed Original ResearchConceptsInitiator methionineNull allelesErythrocyte membrane protein genesMembrane protein geneRabbit reticulocyte lysateTissue-specific promotersErythrocyte membrane skeletonExon 1 sequencesIsoform diversityAlternative splicingTranslation initiationProtein geneAnkyrin geneMembrane skeletonAlternate polyadenylationPlasma membraneReticulocyte lysateMethionine mutationCOOH terminusErythroid cellsDownstream codonsGenomic DNANumerous isoformsAnkyrinGenes
2007
Alternate Promoters Direct Tissue-Specific Expression of Erythrocyte Ankyrin Transcripts with Novel NH2-Termini.
Steiner L, Lin J, Owens A, Sangerman J, Bodine D, Gallagher P. Alternate Promoters Direct Tissue-Specific Expression of Erythrocyte Ankyrin Transcripts with Novel NH2-Termini. Blood 2007, 110: 1710. DOI: 10.1182/blood.v110.11.1710.1710.Peer-Reviewed Original ResearchExon 1AExon 1bInitiator methionineFirst exonKb downstreamErythroid cellsNH2 terminusDNase I hypersensitive site mappingDirect tissue-specific expressionAmino acidsHypersensitive site mappingTissue-restricted patternTissue-specific expressionExon 1Alternate first exonsQuantitative RT-PCR analysisGATA-1 sitesTissue-specific promotersGene expression studiesLuciferase expressionExon 2 sequencesSkeletal muscleIsoform diversityAnkyrin repeatsAnkyrin-1 mutationsA GC Box Element Is Critical for Transcriptional Regulation of the K-Cl Cotransporter Isoform KCC3a in Hematopoetic Cells.
Crable S, Joiner C, Gallagher P. A GC Box Element Is Critical for Transcriptional Regulation of the K-Cl Cotransporter Isoform KCC3a in Hematopoetic Cells. Blood 2007, 110: 1712. DOI: 10.1182/blood.v110.11.1712.1712.Peer-Reviewed Original ResearchUntranslated sequenceInitiator methionineErythroid cellsNovel transcriptsPromoter activityExon 1Cell RNATerminal truncationExon 1DTrans-regulatory factorsMajor splicing isoformsPotential phosphorylation sitesErythroid K562 cellsGC-box elementsFull promoter activityPrimer extension analysisTranslation initiation siteLate erythroid cellsFurther deletion analysisVolume regulationKCl cotransporterLuciferase promoter constructsBone marrow RNATranscriptional regulationEST database
2006
Alternative Splicing within Exon 1 of the KCl Cotransporter-3 (KCC3) Gene Results in Novel Transcripts in Erythroid Cells.
Joiner C, Crable S, Gallagher P. Alternative Splicing within Exon 1 of the KCl Cotransporter-3 (KCC3) Gene Results in Novel Transcripts in Erythroid Cells. Blood 2006, 108: 1564. DOI: 10.1182/blood.v108.11.1564.1564.Peer-Reviewed Original ResearchUntranslated sequenceNovel transcriptsInitiator methionineErythroid cellsAlternative splicingPromoter activityExon 1Cell RNATerminal truncationPotential phosphorylation sitesMajor splicing isoformsErythroid K562 cellsPrimer extension analysisTranslation initiation siteVolume regulationNegative regulatory elementKCl cotransporterLuciferase promoter constructsBone marrow RNAEST databaseTranscriptional controlGenomic regionsPhosphorylation sitesSplicing isoformsDifferent transcripts