Lentivirus Vectors Containing a Band 3/γ-Globin Gene Flanked by Distinct Insulator Elements Are Resistant to Gene Silencing in Primary Mouse Erythroid Cells.
Harrow F, Frazar T, Seidel N, Gallagher P, Bodine D. Lentivirus Vectors Containing a Band 3/γ-Globin Gene Flanked by Distinct Insulator Elements Are Resistant to Gene Silencing in Primary Mouse Erythroid Cells. Blood 2006, 108: 3260. DOI: 10.1182/blood.v108.11.3260.3260.Peer-Reviewed Original ResearchΓ-globin geneLocus control regionInsulator elementsΓ-globinΓ-globin mRNAB3 promoterStart siteΓ-globin gene expressionMouse hematopoietic progenitor cellsLike globin genesAbundant membrane proteinErythroid-specific expressionTranscription start siteΓ-globin expressionMouse erythroid cellsCorrect start sitesHigh-throughput real-time PCRHypersensitive site 4Transgenic miceΒ-globin geneMultiple upstream sitesInsulator activityGlobin genesHematopoietic stem cellsMembrane proteins