2019
Identification and transcriptome analysis of erythroblastic island macrophages
Li W, Wang Y, Zhao H, Zhang H, Xu Y, Wang S, Guo X, Huang Y, Zhang S, Han Y, Wu X, Rice CM, Huang G, Gallagher PG, Mendelson A, Yazdanbakhsh K, Liu J, Chen L, An X. Identification and transcriptome analysis of erythroblastic island macrophages. Blood 2019, 134: 480-491. PMID: 31101625, PMCID: PMC6676133, DOI: 10.1182/blood.2019000430.Peer-Reviewed Original ResearchConceptsErythroblastic islandsEBI macrophagesErythroid cellsErythroblastic island macrophagesGene expression profilesTranscriptome analysisNonerythroid cellsMacrophage functionHematopoietic nicheExpression profilesSpecialized functionsCentral macrophageKnockin mouse modelFlow cytometry analysisEpoRKey moleculesIron recyclingBone marrowCytometry analysisFetal liverNicheEfficient erythropoiesisErythropoiesisIron sourceImportant resource
2014
Global transcriptome analyses of human and murine terminal erythroid differentiation
An X, Schulz VP, Li J, Wu K, Liu J, Xue F, Hu J, Mohandas N, Gallagher PG. Global transcriptome analyses of human and murine terminal erythroid differentiation. Blood 2014, 123: 3466-3477. PMID: 24637361, PMCID: PMC4041167, DOI: 10.1182/blood-2014-01-548305.Peer-Reviewed Original ResearchConceptsTerminal erythroid differentiationErythroid differentiationGene expressionMurine terminal erythroid differentiationStage-specific transcriptomesDifferentiation stageGlobal transcriptome analysisStage-specific patternsRNA sequencing analysisGene expression profilesDistinct developmental stagesMurine transcriptomesFluorescence-activated cell sortingTranscriptional spaceErythroid developmentMurine erythroblastsTranscriptome analysisUnique transcriptomeBioinformatics analysisPerturbed erythropoiesisTranscriptomeExpression profilesErythroid disordersDevelopmental stagesSequencing analysis
2011
Single-lineage transcriptome analysis reveals key regulatory pathways in primitive erythroid progenitors in the mouse embryo
Isern J, He Z, Fraser ST, Nowotschin S, Ferrer-Vaquer A, Moore R, Hadjantonakis AK, Schulz V, Tuck D, Gallagher PG, Baron MH. Single-lineage transcriptome analysis reveals key regulatory pathways in primitive erythroid progenitors in the mouse embryo. Blood 2011, 117: 4924-4934. PMID: 21263157, PMCID: PMC3100699, DOI: 10.1182/blood-2010-10-313676.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCell LineageCytokinesDNA PrimersEpsilon-GlobinsErythroid Precursor CellsErythropoiesisFemaleGene Expression ProfilingGene Expression Regulation, DevelopmentalGene Regulatory NetworksGlycolysisGreen Fluorescent ProteinsGrowth SubstancesMaleMiceMice, Inbred ICRMice, TransgenicOxygenPregnancyRecombinant Fusion ProteinsRNA, MessengerSignal TransductionConceptsPrimitive erythroid progenitorsMouse embryosErythroid progenitorsGlobal expression profilesEmbryonic day 7.5Critical regulatory factorKey regulatory pathwaysOnset of circulationFirst transcriptomeRemarkable proliferative capacityTranscript diversityTransgenic reporterTranscriptome analysisFirst cell typeRegulatory pathwaysHematopoietic lineagesExpression profilesRegulatory factorsCell typesDay 7.5EmbryosProgenitorsYolk sacBlood progenitorsGlycolytic profile