2018
Acid Stimulation of the Citrate Transporter NaDC-1 Requires Pyk2 and ERK1/2 Signaling Pathways
Zacchia M, Tian X, Zona E, Alpern RJ, Preisig PA. Acid Stimulation of the Citrate Transporter NaDC-1 Requires Pyk2 and ERK1/2 Signaling Pathways. Journal Of The American Society Of Nephrology 2018, 29: 1720-1730. PMID: 29678998, PMCID: PMC6054333, DOI: 10.1681/asn.2017121268.Peer-Reviewed Original ResearchMeSH KeywordsAcidsAmmonium ChlorideAnimalsCells, CulturedCitric AcidCSK Tyrosine-Protein KinaseDicarboxylic Acid TransportersEndothelin-1Epithelial CellsFocal Adhesion Kinase 2Kidney Tubules, ProximalMAP Kinase Signaling SystemMiceMice, KnockoutMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3OpossumsOrganic Anion Transporters, Sodium-DependentPhosphorylationProto-Oncogene Proteins c-rafReceptor, Endothelin BRibosomal Protein S6 Kinases, 90-kDaSrc-Family KinasesTransfectionConceptsET-1/ETEndothelin-1Proximal tubulesCultured opossum kidney cellsAcid stimulationInhibition of Pyk2ET-1 stimulationRenal proximal tubulesNaDC-1Opossum kidney cellsCl loadingC-SrcPhosphorylation of Raf1Urine citratePyk2 knockdownEnd pointAcid loadApical NaERK1/2 pathwayStimulationMiceKidney cellsKidneyPyk2ERK1/2
2017
Tough decisions about protocol review
Preisig PA, Silverman J, Brown P, Petervary N. Tough decisions about protocol review. Lab Animal 2017, 46: 290-294. PMID: 28644450, DOI: 10.1038/laban.1300.Commentaries, Editorials and LettersThe role of the IACUC in ensuring research reproducibility
Silverman J, Macy J, Preisig PA. The role of the IACUC in ensuring research reproducibility. Lab Animal 2017, 46: 129-135. PMID: 28328872, DOI: 10.1038/laban.1213.Commentaries, Editorials and Letters
2015
Response to Protocol Review Scenario: One wrong, one right
Preisig PA, Macy JD. Response to Protocol Review Scenario: One wrong, one right. Lab Animal 2015, 44: 87-88. PMID: 25693102, DOI: 10.1038/laban.705.Commentaries, Editorials and Letters
2011
Drosophila: a fruitful model for calcium oxalate nephrolithiasis?
Knauf F, Preisig PA. Drosophila: a fruitful model for calcium oxalate nephrolithiasis? Kidney International 2011, 80: 327-329. PMID: 21799502, DOI: 10.1038/ki.2011.166.Peer-Reviewed Original Research
2010
Acid regulation of NaDC-1 requires a functional endothelin B receptor
Liu L, Zacchia M, Tian X, Wan L, Sakamoto A, Yanagisawa M, Alpern RJ, Preisig PA. Acid regulation of NaDC-1 requires a functional endothelin B receptor. Kidney International 2010, 78: 895-904. PMID: 20703215, DOI: 10.1038/ki.2010.264.Peer-Reviewed Original ResearchMeSH KeywordsAcidosisAnimalsBiological TransportCell LineDicarboxylic Acid TransportersDisease Models, AnimalEndothelin B Receptor AntagonistsEndothelin-1Hydrogen-Ion ConcentrationKidneyMiceMice, KnockoutMicrovilliOligopeptidesOpossumsOrganic Anion Transporters, Sodium-DependentPiperidinesProtein Structure, TertiaryReceptor, Endothelin AReceptor, Endothelin BRecombinant Fusion ProteinsSignal TransductionSymportersTime FactorsTransfectionConceptsEndothelin-1Endothelin-B receptor antagonistFunctional endothelin B receptorsStimulatory effectAcid-stimulatory effectET-1 effectsB receptor antagonistNHE3 activityEndothelin B receptorWild-type miceET-1 stimulationMajor physiological stimulusNaDC-1Acid ingestionReceptor antagonistCitrate reabsorptionReceptor expressionCitrate excretionOpossum kidneyAcid loadB receptorAcid feedingPhysiological stimuliStimulationMiceLow urinary citrate: an overview.
Zacchia M, Preisig P. Low urinary citrate: an overview. Journal Of Nephrology 2010, 23 Suppl 16: s49-56. PMID: 21170889.Peer-Reviewed Original ResearchConceptsUrinary citrate excretionCitrate excretionStone formationCalcium stone formationProximal tubule cellsAcid-base statusKidney stone formationRisk factorsFiltered loadImportant physiological determinantGlomerular filtrateTubule cellsAcid loadExcretionAlkaline loadCitrate levelsHypocitraturiaMetabolic processesMitochondrial metabolic processesPhysiological determinantsMetabolismMitochondrial metabolismCitrate metabolismNaDC1
2009
Biochemical and histological assessment of Alkali therapy during high animal protein intake in the rat
Zerwekh JE, Zou L, Pak CY, Moe OW, Preisig PA. Biochemical and histological assessment of Alkali therapy during high animal protein intake in the rat. Bone 2009, 45: 1004-1009. PMID: 19651255, PMCID: PMC2753741, DOI: 10.1016/j.bone.2009.07.077.Peer-Reviewed Original ResearchConceptsHigh animal protein intakeAnimal protein intakeAlkali therapyBone histomorphometryBone turnoverProtein intakeAcid loadChronic acid loadMajor skeletal effectCancellous bone volumeHigh casein dietNegative calcium balanceDynamic bone histomorphometryLow urinary pHBone formation rateDietary animal protein intakeStatic bone histomorphometryNet acid excretionLow caseinSubstantial acid loadOsteoclastic surfaceWesternized dietUrinary pHUrine biochemistryBone resorption
2007
The acid-activated signaling pathway: Starting with Pyk2 and ending with increased NHE3 activity
Preisig PA. The acid-activated signaling pathway: Starting with Pyk2 and ending with increased NHE3 activity. Kidney International 2007, 72: 1324-1329. PMID: 17882150, DOI: 10.1038/sj.ki.5002543.Peer-Reviewed Original ResearchConceptsTypical Western dietSystemic acid-base balanceMultiple nephron segmentsProximal tubule cellsAcid-base balanceNa/H antiporterWestern dietBicarbonate reclamationProximal tubulesTubule cellsNephron segmentsAcid loadAmmonium secretionLuminal protonsNHE3 activityH antiporterSecretionNHE3Titratable acidRhoA required for acid-induced stress fiber formation and trafficking and activation of NHE3
Yang X, Huang HC, Yin H, Alpern RJ, Preisig PA. RhoA required for acid-induced stress fiber formation and trafficking and activation of NHE3. American Journal Of Physiology. Renal Physiology 2007, 293: f1054-f1064. PMID: 17686951, DOI: 10.1152/ajprenal.00295.2007.Peer-Reviewed Original ResearchMeSH KeywordsAmidesAnimalsBenzoquinonesCell LineEnzyme InhibitorsExocytosisFocal Adhesion Kinase 1Hydrochloric AcidHydrogen-Ion ConcentrationLactams, MacrocyclicOpossumsPaxillinPhosphorylationProtein-Tyrosine KinasesPyridinesRhoA GTP-Binding ProteinRifabutinSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersStress FibersTyrosineConceptsStress fiber formationTyrosine phosphorylationFiber formationFocal adhesion proteinsApical membraneRho-kinaseNHE3 activityExocytic traffickingActivation of NHE3Exocytic processFocal adhesionsNHE3 exocytosisAdhesion proteinsStress fibersProtein abundancePaxillinPhosphorylationMicrofilament structureAntiporter activityKinaseRho-kinase inhibitorKinase inhibitorsTraffickingAbundanceNHE3 activation
2006
Dual role of citrate in mammalian urine
Moe OW, Preisig PA. Dual role of citrate in mammalian urine. Current Opinion In Nephrology & Hypertension 2006, 15: 419-424. PMID: 16775456, DOI: 10.1097/01.mnh.0000232882.35469.72.Peer-Reviewed Original Research
2005
Hypothesizing on the evolutionary origins of salt-induced hypercalciuria
Moe OW, Preisig PA. Hypothesizing on the evolutionary origins of salt-induced hypercalciuria. Current Opinion In Nephrology & Hypertension 2005, 14: 368-372. PMID: 15931006, DOI: 10.1097/01.mnh.0000172724.29628.73.Peer-Reviewed Original Research
2004
Pyk2 activation is integral to acid stimulation of sodium/hydrogen exchanger 3
Li S, Sato S, Yang X, Preisig PA, Alpern RJ. Pyk2 activation is integral to acid stimulation of sodium/hydrogen exchanger 3. Journal Of Clinical Investigation 2004, 114: 1782-1789. PMID: 15599403, PMCID: PMC535061, DOI: 10.1172/jci18046.Peer-Reviewed Original ResearchAcidsAdenosine TriphosphateADP-Ribosylation FactorsAnimalsCell LineCell-Free SystemFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesGenes, DominantGTPase-Activating ProteinsHydrogen-Ion ConcentrationImmunoblottingImmunoprecipitationKidney TubulesMutationOpossumsPhosphorylationProtein BindingProtein-Tyrosine KinasesRatsRats, Sprague-DawleyRNARNA, Small InterferingSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersTime FactorsTransfectionA consensus sequence in the endothelin-B receptor second intracellular loop is required for NHE3 activation by endothelin-1
Laghmani K, Sakamoto A, Yanagisawa M, Preisig PA, Alpern RJ. A consensus sequence in the endothelin-B receptor second intracellular loop is required for NHE3 activation by endothelin-1. American Journal Of Physiology. Renal Physiology 2004, 288: f732-f739. PMID: 15598844, DOI: 10.1152/ajprenal.00300.2004.Peer-Reviewed Original ResearchConceptsEndothelin-1Second intracellular loopET-1 stimulationNHE3 activityIntracellular loopEndothelin-A receptorAdenylyl cyclase inhibitionET-1 regulationProximal tubule apical membraneET receptorsCyclase inhibitionOKP cellsNHE3 activationOpossum kidneyExchanger 3ReceptorsStimulationProtein tyrosine phosphorylationReceptor chimerasPresent studyCellsAdditional mechanismTyrosine phosphorylationApical membraneSimilar patternAn autocrine role for endothelin-1 in the regulation of proximal tubule NHE3
Licht C, Laghmani K, Yanagisawa M, Preisig PA, Alpern RJ. An autocrine role for endothelin-1 in the regulation of proximal tubule NHE3. Kidney International 2004, 65: 1320-1326. PMID: 15086471, DOI: 10.1111/j.1523-1755.2004.00506.x.Peer-Reviewed Original ResearchConceptsChronic metabolic acidosisMetabolic acidosisReverse transcription-polymerase chain reactionProximal tubulesKidney cortexCompetitive reverse transcription-polymerase chain reactionProximal tubule NHE3NHE3 activityPreproET-1 expressionEndothelin B receptorEndothelin-1 expressionET-1 expressionPreproET-1 mRNA abundancePreproET-1 mRNA expressionTranscription-polymerase chain reactionProximal tubule cellsRenal proximal tubulesMRNA abundanceEndothelin-1Control ratsAbdominal aortaAutocrine roleAcidosisTubule cellsAutocrine regulationOKP cells express the Na-dicarboxylate cotransporter NaDC-1
Aruga S, Pajor AM, Nakamura K, Liu L, Moe OW, Preisig PA, Alpern RJ. OKP cells express the Na-dicarboxylate cotransporter NaDC-1. American Journal Of Physiology - Cell Physiology 2004, 287: c64-c72. PMID: 14973148, DOI: 10.1152/ajpcell.00061.2003.Peer-Reviewed Original ResearchConceptsCitrate reabsorptionOKP cellsProximal tubulesDependent citrate uptakeOpossum kidney cell lineUrinary citrate concentrationChronic metabolic acidosisRate of reabsorptionRenal proximal tubulesMetabolic acidosisStone formationKidney stonesKidney cell lineCotransporter 1ReabsorptionCell linesCitrate transportMRNA abundanceXenopus oocytesAcid regulationTubulesNaDC-1Citrate uptakeSuccinate transportHypocitraturiaDietary acid, endothelins, and sleep.
Alpern RJ, Preisig PA. Dietary acid, endothelins, and sleep. Transactions Of The American Clinical And Climatological Association 2004, 115: 385-93; discussion 393-4. PMID: 17060981, PMCID: PMC2263788.Peer-Reviewed Original ResearchConceptsTrafficking of NHE3Proximal tubule cell lineNHE3 activityTubule cell lineSecond intracellular loopEndothelin expressionDietary acidNHE3 abundanceActivation of NHE3OKP cellsProximal tubulesNHE3 mRNAApical membraneHomeostatic responseProtein expressionC-fosReceptor specificityIntracellular loopReceptorsEndothelinCell linesC-JunNHE3C-SrcAcid signaling
2003
Effect of high protein diet on stone-forming propensity and bone loss in rats
Amanzadeh J, Gitomer WL, Zerwekh JE, Preisig PA, Moe OW, Pak CY, Levi M. Effect of high protein diet on stone-forming propensity and bone loss in rats. Kidney International 2003, 64: 2142-2149. PMID: 14633136, DOI: 10.1046/j.1523-1755.2003.00309.x.Peer-Reviewed Original ResearchConceptsHigh casein dietHigh casein groupBone lossCasein groupCasein dietHigh-protein dietProtein dietPathogenesis of hypercalciuriaSerum calcitriol concentrationsStone-forming propensityUrinary calcium excretionBrush border membraneRisk of nephrolithiasisUrinary citrate excretionHigh protein intakeLow casein dietHigher urinary volumeResponse of kidneyNet acid excretionSubstantial acid loadKidney stone formationDays of studyCalcium excretionCalcitriol concentrationsCortical brush border membrane
2002
Compensatory renal hypertrophy is mediated by a cell cycle-dependent mechanism
Liu B, Preisig PA. Compensatory renal hypertrophy is mediated by a cell cycle-dependent mechanism. Kidney International 2002, 62: 1650-1658. PMID: 12371965, DOI: 10.1046/j.1523-1755.2002.00620.x.Peer-Reviewed Original ResearchConceptsCdk2/cyclin E kinase activityCyclin E kinase activityTubule hypertrophyDay 4BrdU incorporationDevelopment of hypertrophyCyclin D kinaseCompensatory renal hypertrophyCell cycle-dependent mechanismProximal tubule proteinsTubule growthHypertrophic formUninephrectomized animalsNephrectomized ratsRenal hypertrophyC57BL6 miceRenal cortexKinase activityDay 2Proximal tubulesHypertrophyHypertrophy markersRatsKinase inhibitorsMiceRole of c-SRC and ERK in acid-induced activation of NHE3
Tsuganezawa H, Sato S, Yamaji Y, Preisig PA, Moe OW, Alpern RJ. Role of c-SRC and ERK in acid-induced activation of NHE3. Kidney International 2002, 62: 41-50. PMID: 12081562, DOI: 10.1046/j.1523-1755.2002.00418.x.Peer-Reviewed Original ResearchMeSH KeywordsAcidosisAmmonium ChlorideAnimalsCells, CulturedCSK Tyrosine-Protein KinaseEnzyme ActivationJNK Mitogen-Activated Protein KinasesMaleMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesPhosphorylationProtein Serine-Threonine KinasesProtein-Tyrosine KinasesProto-Oncogene Proteins c-fosRatsRats, Sprague-DawleySodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSrc-Family KinasesConceptsOKP cellsAcid-induced activationNHE3 activityERK/c-fosC-fos expressionC-Fos activationCytoplasmic pH (pHi) recoveryC-SrcRenal proximal tubulesAcid incubationActivation of NHE3Renal epithelial cellsCultured renal epithelial cellsProximal tubulesChronic acidosisInhibition of MEKNHE3 activationInhibited activationNormal levelsAcid loadImmune complex kinase assayERK kinaseAcidosisPH recoveryEpithelial cells