Improved methods for RNAseq-based alternative splicing analysis
Halperin RF, Hegde A, Lang JD, Raupach EA, Legendre C, Liang W, LoRusso P, Sekulic A, Sosman J, Trent J, Rangasamy S, Pirrotte P, Schork N. Improved methods for RNAseq-based alternative splicing analysis. Scientific Reports 2021, 11: 10740. PMID: 34031440, PMCID: PMC8144374, DOI: 10.1038/s41598-021-89938-2.Peer-Reviewed Original ResearchConceptsProtein-level effectsSplicing analysisSplice eventsSplice isoformsRNAseq dataAlternative splicing analysisTissue-specific splice variantsDifferential splicing analysisGene expression levelsPathogenic splicing variantProtein level expressionSequence readsSplicing variantsSplice variantsOncogenic mutationsMass spectrometry dataSplice alterationsExpression levelsRNAseqIsoformsMelanoma datasetSpectrometry dataNovel statistical approachAnalysis resourcesMass spectrometry