2024
Lateral expansion of the mammalian cerebral cortex is related to anchorage of centrosomes in apical neural progenitors
Morozov Y, Rakic P. Lateral expansion of the mammalian cerebral cortex is related to anchorage of centrosomes in apical neural progenitors. Cerebral Cortex 2024, 34: bhae293. PMID: 39024157, DOI: 10.1093/cercor/bhae293.Peer-Reviewed Original ResearchConceptsNeural progenitor cellsProgenitor cellsVentricular zoneCerebral cortexBasolateral cell membraneApical anchorageProlonged neurogenesisMammalian cerebral cortexPrimary ciliaApical neural progenitorsCell membraneFraction of cellsNeural progenitorsStem cellsCerebral neurogenesisApical segmentsDevelopment of ciliaNuclear translocationMicrotubule organizing centerNeurogenesisCellsMacaque monkeysSpecies-specific differencesCortexBasal bodies
2023
Alternative splicing events as peripheral biomarkers for motor learning deficit caused by adverse prenatal environments
Dutta D, Sasaki J, Bansal A, Sugai K, Yamashita S, Li G, Lazarski C, Wang L, Sasaki T, Yamashita C, Carryl H, Suzuki R, Odawara M, Kawasawa Y, Rakic P, Torii M, Hashimoto-Torii K. Alternative splicing events as peripheral biomarkers for motor learning deficit caused by adverse prenatal environments. Proceedings Of The National Academy Of Sciences Of The United States Of America 2023, 120: e2304074120. PMID: 38051767, PMCID: PMC10723155, DOI: 10.1073/pnas.2304074120.Peer-Reviewed Original ResearchLocalization of PDE4D, HCN1 channels, and mGluR3 in rhesus macaque entorhinal cortex may confer vulnerability in Alzheimer’s disease
Datta D, Perone I, Morozov Y, Arellano J, Duque A, Rakic P, van Dyck C, Arnsten A. Localization of PDE4D, HCN1 channels, and mGluR3 in rhesus macaque entorhinal cortex may confer vulnerability in Alzheimer’s disease. Cerebral Cortex 2023, 33: 11501-11516. PMID: 37874022, PMCID: PMC10724870, DOI: 10.1093/cercor/bhad382.Peer-Reviewed Original ResearchConceptsHCN1 channelsTau pathologyGlutamate synapsesEntorhinal cortexCalcium actionInternal calcium releaseEntorhinal cortex stellate cellsDorsolateral prefrontal cortexSusceptible neuronsInitial pathologySelective vulnerabilityEtiological factorsTau phosphorylationStellate cellsAlzheimer's diseaseSpecific neuronsCalcium releasePrefrontal cortexCortexSynapse strengthPathologyCalcium signalingCalbindinDiseaseNeuronsMolecular programs of regional specification and neural stem cell fate progression in macaque telencephalon
Micali N, Ma S, Li M, Kim S, Mato-Blanco X, Sindhu S, Arellano J, Gao T, Shibata M, Gobeske K, Duque A, Santpere G, Sestan N, Rakic P. Molecular programs of regional specification and neural stem cell fate progression in macaque telencephalon. Science 2023, 382: eadf3786. PMID: 37824652, PMCID: PMC10705812, DOI: 10.1126/science.adf3786.Peer-Reviewed Original ResearchOrigin and development of the claustrum in rhesus macaque
Li H, Duque A, Rakic P. Origin and development of the claustrum in rhesus macaque. Proceedings Of The National Academy Of Sciences Of The United States Of America 2023, 120: e2220918120. PMID: 37406098, PMCID: PMC10334778, DOI: 10.1073/pnas.2220918120.Peer-Reviewed Original ResearchDisorder of Golgi Apparatus Precedes Anoxia-Induced Pathology of Mitochondria
Morozov Y, Rakic P. Disorder of Golgi Apparatus Precedes Anoxia-Induced Pathology of Mitochondria. International Journal Of Molecular Sciences 2023, 24: 4432. PMID: 36901863, PMCID: PMC10003327, DOI: 10.3390/ijms24054432.Peer-Reviewed Original ResearchConceptsGolgi apparatusH anoxiaPost-translational protein modificationStomatin-like protein 2Role of mitochondriaEmbryonic mouse brainEmbryonic mouse brain cellsGolgi architectureSecretory traffickingMitochondrial matrixProtein modificationMouse brain cellsMitochondrial malfunctionTrans cisternaeBrain cellsMitochondriaOrganellesProtein 2Mitochondrial disordersLateral ganglionic eminenceEarly eventsComplex pathological changesMouse brainNormal ultrastructureGanglionic eminence
2022
Role of intracortical neuropil growth in the gyrification of the primate cerebral cortex
Rash B, Arellano J, Duque A, Rakic P. Role of intracortical neuropil growth in the gyrification of the primate cerebral cortex. Proceedings Of The National Academy Of Sciences Of The United States Of America 2022, 120: e2210967120. PMID: 36574666, PMCID: PMC9910595, DOI: 10.1073/pnas.2210967120.Peer-Reviewed Original ResearchConceptsOuter subventricular zoneSubcortical white matterCerebral cortexWhite matterFormation of gyriPrimate cerebral cortexMammalian cerebral cortexMarkers of proliferationCortical malformationsCortical plateGlial cellsGyral developmentSubventricular zoneCortical neurogenesisFetal developmentVentricular zoneCortical foldingNeuronal progenitorsGyrificationNeuronal growthNeuropil growthPrimary gyriCortexNeurodevelopmental disordersGyrusNeural Stem Cells in Adult Mammals are not Astrocytes
Velloso F, Shankar S, Parpura V, Rakic P, Levison S. Neural Stem Cells in Adult Mammals are not Astrocytes. ASN Neuro 2022, 14: 17590914221134739. PMID: 36330653, PMCID: PMC9638700, DOI: 10.1177/17590914221134739.Peer-Reviewed Original ResearchConceptsAdult mammalian subventricular zoneNeural stem cellsMammalian subventricular zoneMammalian neural stem cellsComparative transcriptomic analysisDistinct gene expression profilesStem cellsAdult mammalian neural stem cellsGene expression profilesSingle-cell RNAseqFunction of NSCsMurine neural stem cellsSubventricular zoneTranscriptomic analysisExpression profilesAdult murine neural stem cellsCell sortingAdult mammalsFunction of astrocytesCellsNew neuronsSubtypes of astrocytesMammalsRNAseqNiche
2014
Phenotypic differences in hiPSC NPCs derived from patients with schizophrenia
Brennand K, Savas J, Kim Y, Tran N, Simone A, Hashimoto-Torii K, Beaumont K, Kim H, Topol A, Ladran I, Abdelrahim M, Matikainen-Ankney B, Chao S, Mrksich M, Rakic P, Fang G, Zhang B, Yates J, Gage F. Phenotypic differences in hiPSC NPCs derived from patients with schizophrenia. Molecular Psychiatry 2014, 20: 361-368. PMID: 24686136, PMCID: PMC4182344, DOI: 10.1038/mp.2014.22.Peer-Reviewed Original ResearchMeSH KeywordsAdultAnimalsAntipsychotic AgentsCell DifferentiationCell MovementCells, CulturedFemaleGene ExpressionHumansMaleMiceMice, Inbred C57BLMice, TransgenicMitochondriaNeural Cell Adhesion MoleculesNeural Stem CellsOxidative StressPhenotypePluripotent Stem CellsProsencephalonProteomicsReactive Oxygen SpeciesSchizophreniaYoung AdultConceptsHiPSC neural progenitor cellsNeural progenitor cellsHuman-induced pluripotent stem cellsHiPSC-derived neuronsGene expressionGene expression comparisonsStable isotope labelingProteomic mass spectrometry analysisAbnormal gene expressionPluripotent stem cellsOxidative stressCytoskeletal remodelingMass spectrometry analysisCellular phenotypesExpression comparisonsDevelopmental mechanismsIsotope labelingPhenotypic differencesBrainSpan AtlasDisease predispositionAmino acidsScalable assayNPC phenotypeStem cellsProgenitor cells
2010
Gap Junctions/Hemichannels Modulate Interkinetic Nuclear Migration in the Forebrain Precursors
Liu X, Hashimoto-Torii K, Torii M, Ding C, Rakic P. Gap Junctions/Hemichannels Modulate Interkinetic Nuclear Migration in the Forebrain Precursors. Journal Of Neuroscience 2010, 30: 4197-4209. PMID: 20335455, PMCID: PMC2861434, DOI: 10.1523/jneurosci.4187-09.2010.Peer-Reviewed Original Research4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAdenosine TriphosphateAnimalsBoron CompoundsBromodeoxyuridineCalciumCalcium SignalingCarbenoxoloneCell Line, TumorCell MovementCell NucleusCerebral VentriclesChelating AgentsConnexin 43Cyclooxygenase InhibitorsEgtazic AcidEmbryo, MammalianFemaleGap JunctionsGreen Fluorescent ProteinsKi-67 AntigenMeclofenamic AcidMiceNeuroblastomaNeuronsOrgan Culture TechniquesPatch-Clamp TechniquesPlatelet Aggregation InhibitorsPregnancyProsencephalonPyridoxal PhosphateStem Cells
2009
Evolution of the neocortex: a perspective from developmental biology
Rakic P. Evolution of the neocortex: a perspective from developmental biology. Nature Reviews Neuroscience 2009, 10: 724-735. PMID: 19763105, PMCID: PMC2913577, DOI: 10.1038/nrn2719.Peer-Reviewed Original ResearchConceptsEvo-devo approachSpecies-specific adaptationsExtinct common ancestorsNew cell typesSpecies-specific differencesMutations of genesProliferative ventricular zoneMammalian evolutionDistinct migratory pathwaysCommon ancestorDevelopmental biologyCell divisionNew traitsNew cell subtypesNeural stem cellsProtomap hypothesisMouse embryosNew cytoarchitectonic areasTranscriptional factorsUnique cognitive abilitiesGene expressionDifferential enlargementDistinct traitsMigratory pathwaysCortical evolutionIntegration of neuronal clones in the radial cortical columns by EphA and ephrin-A signalling
Torii M, Hashimoto-Torii K, Levitt P, Rakic P. Integration of neuronal clones in the radial cortical columns by EphA and ephrin-A signalling. Nature 2009, 461: 524-528. PMID: 19759535, PMCID: PMC2874978, DOI: 10.1038/nature08362.Peer-Reviewed Original ResearchConceptsNeocortical excitatory neuronsRadial glial fibersColumnar organizationEphA/ephrinCerebral cortexFunctional impairmentNeuronal clonesExcitatory neuronsGlial fibersTangential migrationCortical columnsNeural progenitorsCircuit developmentEphrinProliferative unitsPopulationClonal populationsCortexNeuronsExocyst is involved in polarized cell migration and cerebral cortical development
Letinic K, Sebastian R, Toomre D, Rakic P. Exocyst is involved in polarized cell migration and cerebral cortical development. Proceedings Of The National Academy Of Sciences Of The United States Of America 2009, 106: 11342-11347. PMID: 19549839, PMCID: PMC2708724, DOI: 10.1073/pnas.0904244106.Peer-Reviewed Original ResearchConceptsCell migrationDelivery of vesiclesNeuronal migrationVesicle tetheringExocyst complexMembrane trafficExocytic pathwayProtein complexesExocytic processExocystSpatial regulationEmbryonic developmentVesicle exocytosisSecretory vesiclesPlasma membraneTIRF microscopyPostmitotic cellsCortical neuronal migrationProper developmentCerebral cortical developmentWound assaysExocytosisVesiclesMembraneCellsSelective reduction of neuron number and volume of the mediodorsal nucleus of the thalamus in macaques following irradiation at early gestational ages
Selemon LD, Begović A, Rakic P. Selective reduction of neuron number and volume of the mediodorsal nucleus of the thalamus in macaques following irradiation at early gestational ages. The Journal Of Comparative Neurology 2009, 515: 454-464. PMID: 19459221, PMCID: PMC2716797, DOI: 10.1002/cne.22078.Peer-Reviewed Original ResearchDecision by division: making cortical maps
Rakic P, Ayoub AE, Breunig JJ, Dominguez MH. Decision by division: making cortical maps. Trends In Neurosciences 2009, 32: 291-301. PMID: 19380167, PMCID: PMC3601545, DOI: 10.1016/j.tins.2009.01.007.Peer-Reviewed Original ResearchConceptsNeuronal fate determinationFate determinationFinal mitotic divisionEvolutionary expansionCell determinationMitotic divisionProtomap hypothesisEarly specificationLatest compendiumBiological basisProliferative zoneNeuronal classesNewborn neuronsBroad spectrumDivisionGenesHuman neocortexCortical malformationsNeuronsRadial unitsCompendiumMigrationCerebral wall
2001
Cell Proliferation Without Neurogenesis in Adult Primate Neocortex
Kornack D, Rakic P. Cell Proliferation Without Neurogenesis in Adult Primate Neocortex. Science 2001, 294: 2127-2130. PMID: 11739948, DOI: 10.1126/science.1065467.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAstrocytesBrainBromodeoxyuridineCell DeathCell DivisionCell MovementEndothelium, VascularFemaleFluorescent Antibody TechniqueGlial Fibrillary Acidic ProteinImmunoenzyme TechniquesMacaca fascicularisMacaca mulattaMaleMicroscopy, ConfocalMicroscopy, FluorescenceNeocortexNeuronsNuclear ProteinsTubulinConceptsPrimate neocortexGlial cell markersAdult macaque monkeysNew neuronsNumerous BrdUOlfactory bulbCerebral wallAdult macaquesMacaque monkeysNonneuronal cellsHigher cognitive functionsCell markersCognitive functionNeocortexNeurogenesisTriple labelingCellular mechanismsCell proliferationNeuronsCellsHippocampusNeurocreationism--Making New Cortical Maps
Rakic P. Neurocreationism--Making New Cortical Maps. Science 2001, 294: 1011-1012. PMID: 11691974, DOI: 10.1126/science.294.5544.1011.Peer-Reviewed Original ResearchRequirement of the JIP1 scaffold protein for stress-induced JNK activation
Whitmarsh A, Kuan C, Kennedy N, Kelkar N, Haydar T, Mordes J, Appel M, Rossini A, Jones S, Flavell R, Rakic P, Davis R. Requirement of the JIP1 scaffold protein for stress-induced JNK activation. Genes & Development 2001, 15: 2421-2432. PMID: 11562351, PMCID: PMC312784, DOI: 10.1101/gad.922801.Peer-Reviewed Original ResearchConceptsJIP1 scaffold proteinSignal transduction pathwaysScaffold proteinTransduction pathwaysMAP kinase signal transduction pathwayJNK activationStress-induced JNK activationKinase signal transduction pathwayC-Jun N-terminal kinase (JNK) signal transduction pathwayExposure of cellsHomologous recombinationEnvironmental stressPrimary hippocampal neuronsC-JunAnoxic stressJIP1ProteinPathwayJNKExcitotoxic stressHippocampal neuronsActivationGenesStressCritical componentTelencephalic origin of human thalamic GABAergic neurons
Letinic K, Rakic P. Telencephalic origin of human thalamic GABAergic neurons. Nature Neuroscience 2001, 4: 931-936. PMID: 11528425, DOI: 10.1038/nn0901-931.Peer-Reviewed Original ResearchConceptsNon-primate mammalian speciesHomeodomain-containing proteinMigratory pathwaysThalamic association nucleiVital dye labelingEvolutionary expansionMammalian speciesAssociation nucleiTelencephalic cellsRodent embryosGuidance cuesChemorepulsive cuesMigration assaysOrganotypic slice culturesDye labelingProliferative zoneNon-human primatesGABAergic neuronsDiencephalic neuronsPathwayDorsal thalamusTelencephalic originGanglionic eminenceSlice culturesNeuronsDevelopment of Layer I Neurons in the Primate Cerebral Cortex
Zecevic N, Rakic P. Development of Layer I Neurons in the Primate Cerebral Cortex. Journal Of Neuroscience 2001, 21: 5607-5619. PMID: 11466432, PMCID: PMC6762645, DOI: 10.1523/jneurosci.21-15-05607.2001.Peer-Reviewed Original ResearchConceptsCerebral cortexLayer ILayer I cellsLayer I neuronsPrimate cerebral cortexCajal-Retzius cellsSubpial granular layerGABAergic neuronsGABAergic interneuronsCortical plateI neuronsGanglionic eminenceMacaque monkeysPial surfaceOlfactory primordiumI cellsNeuronsMonth periodLong gestationGranular layerCortexUltrastructural analysisCorticogenesisCellsPrimates