2001
Programmed Cell Death of Developing Mammalian Neurons after Genetic Deletion of Caspases
Oppenheim R, Flavell R, Vinsant S, Prevette D, Kuan C, Rakic P. Programmed Cell Death of Developing Mammalian Neurons after Genetic Deletion of Caspases. Journal Of Neuroscience 2001, 21: 4752-4760. PMID: 11425902, PMCID: PMC6762357, DOI: 10.1523/jneurosci.21-13-04752.2001.Peer-Reviewed Original ResearchConceptsCell deathGenetic deletionExtensive cytoplasmic vacuolizationPro-apoptotic proteasesCaspase familySpecific caspasesChromatin condensationNonapoptotic pathwaysPostmitotic neuronsCaspase-9Mammalian neuronsCell typesDeath processSpecific perturbationsCaspase-3Altered morphologyCaspasesNuclear changesKey membersDeletionUTP nickCytoplasmic vacuolizationElectron microscopic levelTerminal deoxynucleotidyl transferase-mediated biotinylated UTP nickNeuronal populationsBcl-XL–Caspase-9 Interactions in the Developing Nervous System: Evidence for Multiple Death Pathways
Zaidi A, D'Sa-Eipper C, Brenner J, Kuida K, Zheng T, Flavell R, Rakic P, Roth K. Bcl-XL–Caspase-9 Interactions in the Developing Nervous System: Evidence for Multiple Death Pathways. Journal Of Neuroscience 2001, 21: 169-175. PMID: 11150333, PMCID: PMC6762421, DOI: 10.1523/jneurosci.21-01-00169.2001.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisBcl-2-Associated X ProteinBcl-X ProteinCaspase 3Caspase 9CaspasesCells, CulturedCytarabineGanglia, SpinalGenes, LethalHeterozygoteHomozygoteImmunohistochemistryIn Situ Nick-End LabelingLiverMiceMice, KnockoutNervous SystemNeuronsProto-Oncogene ProteinsProto-Oncogene Proteins c-bcl-2TelencephalonTumor Suppressor Protein p53ConceptsGene family membersCaspase-9 deficiencyCaspase-9Telencephalic neural precursor cellsCell deathDouble homozygous mutantsCaspase family membersMultiple death pathwaysNormal nervous system developmentBcl-2Nervous system developmentBax-deficient neuronsNeuronal apoptosisTelencephalic neuronsDeficient embryosNeural precursor cellsDeath pathwaysFamily membersHomozygous mutantsApoptotic pathwayObligate pathwayBcl-xLApoptosis inducersDeficient neuronsTargeted disruptionCaspases and Their Regulation in Apoptosis during Brain Development
Kuan C, Flavell R, Rakic P. Caspases and Their Regulation in Apoptosis during Brain Development. Research And Perspectives In Neurosciences 2001, 75-88. DOI: 10.1007/978-3-662-04333-2_7.Peer-Reviewed Original ResearchJun N-terminal kinasePost-mitotic neuronsMammalian brain developmentCaspase-3 activationCell deathC. elegansCaspase familyDeath gene ced-3Caspase-3Caspase-9Mammalian caspase familyGene ced-3Ectopic cell deathGene ced-9Complex regulation mechanismsNematode C. elegansCell death pathwaysNormal mouse brain developmentN-terminal kinaseBrain developmentCell death cascadeMouse brain developmentCaspase-3 deficiencyCED-3CED-9Normal Programmed Cell Death of Developing Avian and Mammalian Neurons Following Inhibition or Genetic Deletion of Caspases
Oppenheim R, Kuan C, Prevette D, Rakic P, Yaginuma H. Normal Programmed Cell Death of Developing Avian and Mammalian Neurons Following Inhibition or Genetic Deletion of Caspases. Research And Perspectives In Neurosciences 2001, 61-74. DOI: 10.1007/978-3-662-04333-2_6.Peer-Reviewed Original ResearchCell deathAbsence of caspasesProgrammed Cell DeathCaspase family membersPost-mitotic neuronsGenetic deletionCaspase inhibitorsCaspase activityNeuronal PCDMammalian neuronsPCDCaspasesChick embryosDeletionFamily membersMorphological degenerationEmbryosUpstreamNeuronsNormal occurrenceInhibitorsOvoInhibitionMembersDeath
2000
Mechanisms of programmed cell death in the developing brain
Kuan C, Roth K, Flavell R, Rakic P, Kuan C, Roth K, Flavell R, Rakic P. Mechanisms of programmed cell death in the developing brain. Trends In Neurosciences 2000, 23: 291-297. PMID: 10856938, DOI: 10.1016/s0166-2236(00)01581-2.Peer-Reviewed Original ResearchConceptsCell deathNematode Caenorhabditis elegansEarly embryonic developmentGene-targeting studiesCell death pathwaysVertebrate nervous systemMammalian brain developmentCaenorhabditis elegansEmbryonic developmentGenetic analysisPostmitotic neuronsBrain developmentNeural cell deathAnalogous functionsDistinct rolesProgenitor cellsMammalian brainApoptosisNew insightsImportant mechanismElegansHomologLater stagesNervous systemMechanismRadial Unit Hypothesis of Neocortical Expansion
Rakic P. Radial Unit Hypothesis of Neocortical Expansion. Novartis Foundation Symposia 2000, 228: 30-45. PMID: 10929315, DOI: 10.1002/0470846631.ch3.Peer-Reviewed Original ResearchConceptsRadial unit hypothesisSpecies-specific sizeFamily of genesMutations of genesVentricular zoneMammalian evolutionFounder cellsNatural selectionRegulatory genesCell divisionMorphoregulatory moleculesPostmitotic cellsTransgenic animalsCortical plateCell deathNeocortical expansionGenesBasic organizationGlial scaffoldingColumnar unitsCortical cellsCerebral cortexCellsCortical developmentSynaptic connectionsCaspase‐3 is required for apoptosis‐associated DNA fragmentation but not for cell death in neurons deprived of potassium
D'Mello S, Kuan C, Flavell R, Rakic P. Caspase‐3 is required for apoptosis‐associated DNA fragmentation but not for cell death in neurons deprived of potassium. Journal Of Neuroscience Research 2000, 59: 24-31. PMID: 10658182, DOI: 10.1002/(sici)1097-4547(20000101)59:1<24::aid-jnr4>3.0.co;2-8.Peer-Reviewed Original ResearchConceptsCell deathCaspase-3Apoptosis-associated DNA fragmentationDNA fragmentationCell death pathwaysRegulated cell deathPan-caspase inhibitorApoptosis-inducing stimuliDeath pathwaysChromatin condensationCaspase inhibitorsCaspase inhibitionApoptotic featuresCerebellar granule neuronsPotassium deprivationFmkCultured cerebellar granule neuronsCaspasesCrucial effectorNonneuronal cellsApoptosisSame extentGranule neuronsPivotal roleNeuronal deathProgrammed Cell Death in Mouse Brain Development
Kuan C, Flavell R, Rakic P. Programmed Cell Death in Mouse Brain Development. Results And Problems In Cell Differentiation 2000, 30: 145-162. PMID: 10857188, DOI: 10.1007/978-3-540-48002-0_6.Peer-Reviewed Original ResearchConceptsCell death machineryMouse brain developmentCell deathDeath machineryNematode Caenorhabditis elegansBasic cellular eventsIdeal experimental systemVertebrate nervous systemBrain developmentCaenorhabditis elegansNormal human brain developmentNerve growth factorGenetic pathwaysTrophic theoryRecent geneSpecific genesCellular eventsGenetic studiesNeural developmentHuman brain developmentTargeted disruptionHigher primatesMechanistic understandingEssential roleGenes
1999
Organotypic slice cultures for analysis of proliferation, cell death, and migration in the embryonic neocortex1Published on the World Wide Web on 8 July 1999.1
Haydar T, Bambrick L, Krueger B, Rakic P. Organotypic slice cultures for analysis of proliferation, cell death, and migration in the embryonic neocortex1Published on the World Wide Web on 8 July 1999.1. Brain Research 1999, 4: 425-437. PMID: 10592354, DOI: 10.1016/s1385-299x(99)00033-1.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisBromodeoxyuridineCell DivisionCell MovementCell SurvivalFemaleFetusFluorescent Antibody TechniqueGlial Fibrillary Acidic ProteinIn Situ Nick-End LabelingMiceMice, Inbred ICRMicroscopy, ConfocalMicrotubule-Associated ProteinsNeocortexNeurogliaNeuronsOrgan Culture TechniquesPregnancyConceptsCell deathProper cortical developmentCell-cell interactionsDynamic cellular interactionsQuantitative confocal microscopyDiffusible regulatorsPrimary cell culturesAnalysis of proliferationCellular interactionsCellular mechanismsCortical neurogenesisNeuronal migrationConfocal microscopyCell proliferationNeocortical neurogenesisExperimental manipulationThree-dimensional environmentVivo processesCell culturesNeurogenesisPhysiological methodsTropic supportOrganotypic slice culturesProliferationCortical developmentThe Jnk1 and Jnk2 Protein Kinases Are Required for Regional Specific Apoptosis during Early Brain Development
Kuan C, Yang D, Roy D, Davis R, Rakic P, Flavell R. The Jnk1 and Jnk2 Protein Kinases Are Required for Regional Specific Apoptosis during Early Brain Development. Neuron 1999, 22: 667-676. PMID: 10230788, DOI: 10.1016/s0896-6273(00)80727-8.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisBrainCalcium-Calmodulin-Dependent Protein KinasesCaspasesEmbryonic and Fetal DevelopmentEnzyme ActivationGene Expression Regulation, DevelopmentalGene Expression Regulation, EnzymologicJNK Mitogen-Activated Protein KinasesMiceMice, KnockoutMitogen-Activated Protein Kinase 9Mitogen-Activated Protein KinasesNervous SystemProtein KinasesRhombencephalonConceptsNeural tube closureBrain developmentEmbryonic lethalDouble mutantKinase familyCompound mutantsProtein kinaseCaspase activationPrecocious degenerationJNK2 geneTube closureEarly brain developmentCell deathJNK1Different membersSpecific apoptosisApoptosisMutantsMutant miceJNK2JNK3Mutant forebrainSevere dysregulationFamilyKinase
1996
Decreased apoptosis in the brain and premature lethality in CPP32-deficient mice
Kuida K, Zheng T, Na S, Kuan C, Yang D, Karasuyama H, Rakic P, Flavell R. Decreased apoptosis in the brain and premature lethality in CPP32-deficient mice. Nature 1996, 384: 368-372. PMID: 8934524, DOI: 10.1038/384368a0.Peer-Reviewed Original ResearchConceptsCED-3Protease familyMajor morphogenetic changesProgrammed Cell DeathICE protease familyMutant embryosCaenorhabditis elegansDeath genesMorphogenetic cellApoptotic stimuliHomologous recombinationMorphogenetic changesMendelian geneticsSequence homologyHigh similarityCell deathPremature lethalitySupernumerary cellsEmbryonic day 12Mammalian brainCPP32Critical rolePostnatal stagesApoptosisBrain development
1981
Neurogenetic gradients in the superior and inferior colliculi of the rhesus monkey
Cooper M, Rakic P. Neurogenetic gradients in the superior and inferior colliculi of the rhesus monkey. The Journal Of Comparative Neurology 1981, 202: 309-334. PMID: 7298901, DOI: 10.1002/cne.902020303.Peer-Reviewed Original ResearchConceptsSuperior colliculusInferior colliculusCentral nucleusInferior colliculiRhesus monkeysVentrodorsal gradientLateral-medial gradientAnterior-posterior gradientTectal neuronsNeurogenetic gradientsPeak proliferationSubventricular zoneSelective cell deathRostrocaudal gradientCNS cellsFinal mitosisNeuronsDistinctive spatiotemporal patternsNeurogenesisEmbryonic dayColliculiColliculusGestation periodCell deathLateral cell movement
1973
Weaver Mutant Mouse Cerebellum: Defective Neuronal Migration Secondary to Abnormality of Bergmann Glia
Rakic P, Sidman R. Weaver Mutant Mouse Cerebellum: Defective Neuronal Migration Secondary to Abnormality of Bergmann Glia. Proceedings Of The National Academy Of Sciences Of The United States Of America 1973, 70: 240-244. PMID: 4509657, PMCID: PMC433223, DOI: 10.1073/pnas.70.1.240.Peer-Reviewed Original ResearchConceptsDefective neuronal migrationGene dosage effectPrimary cellular targetSite of genesisGenetic lociCellular targetsPrimary genetic abnormalityCell deathBergmann glial cellsDosage effectCellular levelGranule cell deathNeuronal migrationCell processesCell genesisNeurological mutantPhenotypic expressionBergmann glial processesGlial cellsCell neuronsGenetic abnormalitiesYoung neuronsWv/wvCerebellum of miceGlial abnormalities