Organoid Modeling of the Tumor Immune Microenvironment
Neal JT, Li X, Zhu J, Giangarra V, Grzeskowiak CL, Ju J, Liu IH, Chiou SH, Salahudeen AA, Smith AR, Deutsch BC, Liao L, Zemek AJ, Zhao F, Karlsson K, Schultz LM, Metzner TJ, Nadauld LD, Tseng YY, Alkhairy S, Oh C, Keskula P, Mendoza-Villanueva D, De La Vega FM, Kunz PL, Liao JC, Leppert JT, Sunwoo JB, Sabatti C, Boehm JS, Hahn WC, Zheng GXY, Davis MM, Kuo CJ. Organoid Modeling of the Tumor Immune Microenvironment. Cell 2018, 175: 1972-1988.e16. PMID: 30550791, PMCID: PMC6656687, DOI: 10.1016/j.cell.2018.11.021.Peer-Reviewed Original ResearchConceptsTumor-infiltrating lymphocytesPatient-derived organoidsImmune checkpoint blockadePrimary tumor epitheliumTumor epitheliumTumor microenvironmentAntigen-specific tumor-infiltrating lymphocytesTumor immune microenvironmentAir-liquid interface methodSyngeneic immunocompetent hostsImmune stromaCheckpoint blockadeImmunocompetent hostsImmune microenvironmentNeoplastic epitheliumImmune cellsTumor TTumor cytotoxicityMouse tumorsHuman biopsiesImmune repertoireCancer culturesEpitheliumThree-dimensional organoidsOrganoid modeling