2021
A transgenic system for targeted ablation of reproductive and maternal-effect genes
Bertho S, Kaufman O, Lee K, Santos-Ledo A, Dellal D, Marlow F. A transgenic system for targeted ablation of reproductive and maternal-effect genes. Development 2021, 148 PMID: 34143203, PMCID: PMC8254866, DOI: 10.1242/dev.198010.Peer-Reviewed Original ResearchConceptsMaternal-effect genesForward genetic screensReverse genetics systemEmbryonic developmentRegulation of embryonic developmentTransgenic systemZygotic functionGenetic systemGenetic screeningGene productsMolecular regulationGenesEarly embryosDevelopmental processesNormal embryogenesisLate functionMolecular pathwaysBucky ballEmbryosMutagenesis
2020
Loss of dmrt1 restores zebrafish female fates in the absence of cyp19a1a but not rbpms2a/b
Romano S, Kaufman O, Marlow F. Loss of dmrt1 restores zebrafish female fates in the absence of cyp19a1a but not rbpms2a/b. Development 2020, 147: dev190942. PMID: 32895289, PMCID: PMC7541348, DOI: 10.1242/dev.190942.Peer-Reviewed Original ResearchConceptsSex-specific differentiationFemale fateSex determinationLoss of DMRT1Genetic epistasis experimentsEpistasis experimentsAdult gonadsGenetic hierarchyFate specificationFemale differentiationGenetic evidenceDMRT1Fate acquisitionSomatic tissuesAdult sexMutantsCyp19a1aRepressionRBPMS2DifferentiationFateOocyte maturationGermlineOvaryZebrafish
2018
rbpms2 functions in Balbiani body architecture and ovary fate
Kaufman O, Lee K, Martin M, Rothhämel S, Marlow F. rbpms2 functions in Balbiani body architecture and ovary fate. PLOS Genetics 2018, 14: e1007489. PMID: 29975683, PMCID: PMC6049948, DOI: 10.1371/journal.pgen.1007489.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedCell PolarityCytoplasmEmbryo, NonmammalianFemaleGene Expression Regulation, DevelopmentalGerm CellsMaleMitochondriaMutagenesis, Site-DirectedOocytesOogenesisOvaryRNA-Binding ProteinsSex DifferentiationTumor Suppressor Protein p53ZebrafishZebrafish ProteinsConceptsRNA-binding proteinsPrimordial germ cellsGerm cellsIntact RNA binding domainLocalized to subcellular compartmentsRNA-binding domainGerm granulesProtein domainsElectron-dense cytoplasmic inclusionsMutations of TP53Accumulation of mitochondriaPromote oogenesisRibonucleoprotein granulesSubcellular compartmentsBinding domainAsymmetric accumulationDevelopmental regulationFertile malesBalbiani bodyOocyte differentiationMutantsBlastula cellsSexual differentiationSomatic cellsBody architecture
2016
Chapter 1 Methods to study maternal regulation of germ cell specification in zebrafish
Kaufman O, Marlow F. Chapter 1 Methods to study maternal regulation of germ cell specification in zebrafish. Methods In Cell Biology 2016, 134: 1-32. PMID: 27312489, PMCID: PMC5039941, DOI: 10.1016/bs.mcb.2016.02.001.Peer-Reviewed Original ResearchConceptsGerm cell specificationGerm cell formationCell-specificGerm lineControl of maternal gene productsProcess of germ cell specificationMaternal gene productsGerm cell regulationGerm cell developmentLoss-of-function studiesZebrafish genomeManipulating genomesGenetic tractabilityGenetic regulationGenetic screeningGene productsCell formationMolecular biology techniquesExpression analysisCell developmentMolecular regulationCell regulationEarly embryosIn situ hybridizationGenetic models