2021
A transgenic system for targeted ablation of reproductive and maternal-effect genes
Bertho S, Kaufman O, Lee K, Santos-Ledo A, Dellal D, Marlow F. A transgenic system for targeted ablation of reproductive and maternal-effect genes. Development 2021, 148 PMID: 34143203, PMCID: PMC8254866, DOI: 10.1242/dev.198010.Peer-Reviewed Original ResearchConceptsMaternal-effect genesForward genetic screensReverse genetics systemEmbryonic developmentRegulation of embryonic developmentTransgenic systemZygotic functionGenetic systemGenetic screeningGene productsMolecular regulationGenesEarly embryosDevelopmental processesNormal embryogenesisLate functionMolecular pathwaysBucky ballEmbryosMutagenesis
2018
rbpms2 functions in Balbiani body architecture and ovary fate
Kaufman O, Lee K, Martin M, Rothhämel S, Marlow F. rbpms2 functions in Balbiani body architecture and ovary fate. PLOS Genetics 2018, 14: e1007489. PMID: 29975683, PMCID: PMC6049948, DOI: 10.1371/journal.pgen.1007489.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedCell PolarityCytoplasmEmbryo, NonmammalianFemaleGene Expression Regulation, DevelopmentalGerm CellsMaleMitochondriaMutagenesis, Site-DirectedOocytesOogenesisOvaryRNA-Binding ProteinsSex DifferentiationTumor Suppressor Protein p53ZebrafishZebrafish ProteinsConceptsRNA-binding proteinsPrimordial germ cellsGerm cellsIntact RNA binding domainLocalized to subcellular compartmentsRNA-binding domainGerm granulesProtein domainsElectron-dense cytoplasmic inclusionsMutations of TP53Accumulation of mitochondriaPromote oogenesisRibonucleoprotein granulesSubcellular compartmentsBinding domainAsymmetric accumulationDevelopmental regulationFertile malesBalbiani bodyOocyte differentiationMutantsBlastula cellsSexual differentiationSomatic cellsBody architecture