2001
Improvements in cytogenetic slide preparation: Controlled chromosome spreading, chemical aging and gradual denaturing
Henegariu O, Heerema N, Wright L, Bray‐Ward P, Ward D, Vance G. Improvements in cytogenetic slide preparation: Controlled chromosome spreading, chemical aging and gradual denaturing. Cytometry 2001, 43: 101-109. PMID: 11169574, DOI: 10.1002/1097-0320(20010201)43:2<101::aid-cyto1024>3.0.co;2-8.Peer-Reviewed Original ResearchConceptsComparative genomic hybridizationClassical banding techniquesSitu hybridization applicationsChromosomal integrityChromosome spreadsMultiplex FISHMolecular cytogeneticsFISH applicationsChromosome spreadingFISH techniqueGenomic hybridizationEnvironmental conditionsSitu hybridizationBanding techniquesChromosomesHybridizationReproducible protocolFISH resultsCytogenetic slidesCell suspensionsSlide preparationFishMetaphase
1998
Characterization of Multiple 12p Rearrangements in Testicular Germ Cell Tumor Cell Line 833K and Its Subclone 64CP by Chromosome Microdissection
Blough R, Vance G, Henegariu O, Smolarek T, Sledge G, Heerema N. Characterization of Multiple 12p Rearrangements in Testicular Germ Cell Tumor Cell Line 833K and Its Subclone 64CP by Chromosome Microdissection. Cancer Genetics 1998, 106: 24-29. PMID: 9772905, DOI: 10.1016/s0165-4608(98)00042-9.Peer-Reviewed Original ResearchConceptsChromosome 12 regionDistal 12pNormal human chromosomesBeta-satellite DNABand-specific probesFISH experimentsChromosome arm 12pChromosome microdissectionChromosomal microdissectionHuman chromosomesPainting probesChromosome 12Stalk regionCisplatin-resistant subclonesSitu hybridizationRDNACommon breakpointFishMicrodissectionSpecific originChromatinRearrangementChromosomesUnusual distributionBreakpoints
1997
A case with mosaic di‐, tetra‐, and octacentric ring Y chromosomes
Henegariu O, Pescovitz O, Vance G, Verbrugge J, Heerema N. A case with mosaic di‐, tetra‐, and octacentric ring Y chromosomes. American Journal Of Medical Genetics 1997, 71: 426-429. PMID: 9286449, DOI: 10.1002/(sici)1096-8628(19970905)71:4<426::aid-ajmg10>3.0.co;2-i.Peer-Reviewed Original ResearchConceptsPresence of heterochromatinHigh-resolution G-bandingSpecific banding patternsHuman telomeresSRY locusRing Y chromosomeY chromosomePaint probesY centromereColor FISHBanding patternsPCR analysisUllrich-Turner syndromeTriple-color FISHQ-bandingRing chromosomesG-bandingChromosomesPhallic structuresUrogenital openingFishCentromeresHeterochromatinTelomeresLoci