2024
The effect of acute estrogen suppression on cutaneous endothelium-dependent vasodilation in women with endometriosis
Colburn A, Leone C, Alexander L, Stachenfeld N. The effect of acute estrogen suppression on cutaneous endothelium-dependent vasodilation in women with endometriosis. Physiology 2024, 39: 421. DOI: 10.1152/physiol.2024.39.s1.421.Peer-Reviewed Original ResearchEndothelium-dependent vasodilationEstrogen suppressionHealthy controlsLDF/mean arterial pressureSodium nitroprusside perfusionDose-response curveLaser Doppler flowmetryCardiovascular disease riskCutaneous vascular conductanceACh doseEndometriosis patientsPerfusion of acetylcholineMenstrual cycleIntradermal microdialysisSuppress estrogenEndothelial functionArterial pressureEndometriosisVasodilatory functionDoppler flowmetryVascular conductanceVasodilationMaximal vasodilationGraded perfusionEstrogenInhibition of NF-κB Activation Improves Non-Nitric Oxide-Mediated Cutaneous Microvascular Function in Reproductive-Aged Healthy Women
Content V, Williams A, Stachenfeld N, Alexander L. Inhibition of NF-κB Activation Improves Non-Nitric Oxide-Mediated Cutaneous Microvascular Function in Reproductive-Aged Healthy Women. Physiology 2024, 39: 562. DOI: 10.1152/physiol.2024.39.s1.562.Peer-Reviewed Original ResearchInhibition of NF-kB activationCutaneous microvascular functionNF-kB activationMicrovascular functionHealthy womenCutaneous vascular conductanceSalsalate treatmentInflammatory mechanismsImpact of systemic inflammationIncreasing concentrations of acetylcholineIntradermal microdialysis fibersDose-response protocolCohort of patientsMeasuring cutaneous blood flowPlacebo-controlled designHealthy control subjectsSystemic vascular functionNO synthase inhibitorHuman cutaneous circulationLaser-Doppler flowmetryDose-response curveRed blood cell fluxBlood cell fluxCardiovascular disease riskVentral forearm skin
2022
Seven days of statin treatment improves nitric-oxide mediated endothelial-dependent cutaneous microvascular function in women with endometriosis
Dillon GA, Stanhewicz AE, Serviente C, Flores VA, Stachenfeld N, Alexander LM. Seven days of statin treatment improves nitric-oxide mediated endothelial-dependent cutaneous microvascular function in women with endometriosis. Microvascular Research 2022, 144: 104421. PMID: 35970408, PMCID: PMC9527706, DOI: 10.1016/j.mvr.2022.104421.Peer-Reviewed Original ResearchConceptsEndothelium-dependent vasodilationLaser Doppler fluxEndothelial functionCardiovascular diseaseOral atorvastatinExerts potent anti-inflammatory effectsEndothelium-dependent agonist acetylcholineShort-term statin administrationPotent anti-inflammatory effectsIntradermal microdialysis perfusionNO-dependent dilationImpaired endothelial functionMicrovascular endothelial functionCutaneous microvascular functionCutaneous vascular conductanceAnti-inflammatory effectsNO-dependent vasodilationNO synthase inhibitorDose-response curveStatin therapyAdjunctive therapyAtorvastatin treatmentCVD riskEndothelial dysfunctionStatin administration
2019
Androgens drive microvascular endothelial dysfunction in women with polycystic ovary syndrome: role of the endothelin B receptor
Usselman CW, Yarovinsky T, Steele FE, Leone CA, Taylor HS, Bender JR, Stachenfeld NS. Androgens drive microvascular endothelial dysfunction in women with polycystic ovary syndrome: role of the endothelin B receptor. The Journal Of Physiology 2019, 597: 2853-2865. PMID: 30847930, DOI: 10.1113/jp277756.Peer-Reviewed Original ResearchMeSH KeywordsAdultAndrogensCardiovascular DiseasesDihydrotestosteroneEndothelin-1Endothelium, VascularEstrogensEthinyl EstradiolFemaleGlucose Tolerance TestHumansMicrovesselsNitric OxideObesityPolycystic Ovary SyndromeReceptor, Endothelin BSkinVascular Endothelial Growth Factor AVasodilationYoung AdultConceptsPolycystic ovary syndromeCardiovascular dysfunctionEndothelial dysfunctionOvary syndromeEndothelin-1Endothelial functionNO productionAndrogen receptor-dependent mannerEndothelin-B receptor subtypeCardiovascular risk factorsMicrovascular endothelial dysfunctionMicrovascular endothelial functionCutaneous vascular conductanceB receptor subtypesEndothelin B receptorLaser Doppler flowmetryNitric oxide inhibitionReceptor-dependent mannerEndothelial cell NO productionDose-response curveVasodilating responsePCOS subjectsLean womenObese womenVascular conductance
2017
Microvascular endothelial function in lean versus obese women with polycystic ovary syndrome: role of the endothelin B receptor
Usselman C, Taylor H, Stachenfeld N. Microvascular endothelial function in lean versus obese women with polycystic ovary syndrome: role of the endothelin B receptor. The FASEB Journal 2017, 31 DOI: 10.1096/fasebj.31.1_supplement.691.5.Peer-Reviewed Original ResearchPolycystic ovary syndromeLean polycystic ovary syndromeObese PCOS womenCutaneous vascular conductanceNitric oxide inhibitionObese womenET-1PCOS womenOvary syndromeVasodilatory responseEndothelial functionOxide inhibitionEndothelin receptor subtype BObese polycystic ovary syndromeIntradermal microdialysis perfusionCardiovascular risk factorsMicrovascular endothelial functionPlasma endothelin-1Adverse cardiovascular outcomesAmerican Heart AssociationEndothelin B receptorLaser Doppler flowmetryDose-response curveCardiovascular outcomesIndependent vasodilation
2014
Dichotomous estradiol and genistein effects on cutaneous endothelium‐dependent vasodilation in women with and without insulin resistance (678.6)
Wenner M, Taylor H, Stachenfeld N. Dichotomous estradiol and genistein effects on cutaneous endothelium‐dependent vasodilation in women with and without insulin resistance (678.6). The FASEB Journal 2014, 28 DOI: 10.1096/fasebj.28.1_supplement.678.6.Peer-Reviewed Original ResearchEndothelial-dependent dilationInsulin-resistant womenCutaneous vascular conductanceL-NMMAHealthy womenGenistein effectsEndothelium-dependent vasodilationLaser Doppler flowmetryResistant womenVascular conductanceE2 effectsInsulin resistanceMicrodialysis infusionDependent dilationDoppler flowmetryGEN effectsWomenE2National InstituteGrant funding sourcesC heatingCVCmaxVasodilationCardioprotectionFlowmetry
2013
ETA subtype receptor sensitivity to testosterone contributes to microvascular dysfunction in PCOS
Stachenfeld N, Wenner M, Taylor H. ETA subtype receptor sensitivity to testosterone contributes to microvascular dysfunction in PCOS. The FASEB Journal 2013, 27: 1133.10-1133.10. DOI: 10.1096/fasebj.27.1_supplement.1133.10.Peer-Reviewed Original ResearchPolycystic ovary syndromeCutaneous vascular conductanceHeat-induced vasodilationEndothelium-mediated vasodilationETB receptor antagonistLaser Doppler flowmetryETA blockadeTestosterone contributesEndothelial dysfunctionOvary syndromeVascular conductanceVasoconstrictor toneETA receptorsHormone antagonistReceptor antagonistMicrodialysis infusionSex hormonesDoppler flowmetryReceptor mechanismsReceptor sensitivityDay 8Day 4VasodilationDysfunctionAntagonist