Comparative Genomics Reveals Two Novel RNAi Factors in Trypanosoma brucei and Provides Insight into the Core Machinery
Barnes RL, Shi H, Kolev NG, Tschudi C, Ullu E. Comparative Genomics Reveals Two Novel RNAi Factors in Trypanosoma brucei and Provides Insight into the Core Machinery. PLOS Pathogens 2012, 8: e1002678. PMID: 22654659, PMCID: PMC3359990, DOI: 10.1371/journal.ppat.1002678.Peer-Reviewed Original ResearchMeSH KeywordsArgonaute ProteinsBase SequenceExonucleasesGene Knockout TechniquesGenomicsRNA InterferenceRNA, Small InterferingSequence Analysis, RNATrypanosoma brucei bruceiConceptsRNA interferenceT. bruceiComparative genomicsTrypanosoma bruceiMechanism of RNAiCatalog of genesCore RNAi machineryWide RNAi screenArgonaute 1 proteinNext-generation IlluminaRepertoire of factorsLoss of fitnessDistinct developmental stagesAncient eukaryotesRNAi factorsCore machineryRNAi machineryRNAi screenRNAi toolTrypanosomatid protozoaDuplex siRNAsRNAi efficiencyHuman parasitesMolecular explorationBrucei