2012
Comparative Genomics Reveals Two Novel RNAi Factors in Trypanosoma brucei and Provides Insight into the Core Machinery
Barnes RL, Shi H, Kolev NG, Tschudi C, Ullu E. Comparative Genomics Reveals Two Novel RNAi Factors in Trypanosoma brucei and Provides Insight into the Core Machinery. PLOS Pathogens 2012, 8: e1002678. PMID: 22654659, PMCID: PMC3359990, DOI: 10.1371/journal.ppat.1002678.Peer-Reviewed Original ResearchMeSH KeywordsArgonaute ProteinsBase SequenceExonucleasesGene Knockout TechniquesGenomicsRNA InterferenceRNA, Small InterferingSequence Analysis, RNATrypanosoma brucei bruceiConceptsRNA interferenceT. bruceiComparative genomicsTrypanosoma bruceiMechanism of RNAiCatalog of genesCore RNAi machineryWide RNAi screenArgonaute 1 proteinNext-generation IlluminaRepertoire of factorsLoss of fitnessDistinct developmental stagesAncient eukaryotesRNAi factorsCore machineryRNAi machineryRNAi screenRNAi toolTrypanosomatid protozoaDuplex siRNAsRNAi efficiencyHuman parasitesMolecular explorationBruceiA single-cloning-step procedure for the generation of RNAi plasmids producing long stem–loop RNA
Atayde VD, Ullu E, Kolev NG. A single-cloning-step procedure for the generation of RNAi plasmids producing long stem–loop RNA. Molecular And Biochemical Parasitology 2012, 184: 55-58. PMID: 22542486, PMCID: PMC3358476, DOI: 10.1016/j.molbiopara.2012.04.003.Peer-Reviewed Original Research
2011
RNA Interference in Protozoan Parasites: Achievements and Challenges
Kolev NG, Tschudi C, Ullu E. RNA Interference in Protozoan Parasites: Achievements and Challenges. MSphere 2011, 10: 1156-1163. PMID: 21764910, PMCID: PMC3187059, DOI: 10.1128/ec.05114-11.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsEntamoebaGene Expression RegulationLeishmania braziliensisParasitesPlasmodiumRNA InterferenceRNA, Small InterferingTrypanosomaTrypanosoma brucei brucei
2009
Distinct and overlapping roles for two Dicer-like proteins in the RNA interference pathways of the ancient eukaryote Trypanosoma brucei
Patrick KL, Shi H, Kolev NG, Ersfeld K, Tschudi C, Ullu E. Distinct and overlapping roles for two Dicer-like proteins in the RNA interference pathways of the ancient eukaryote Trypanosoma brucei. Proceedings Of The National Academy Of Sciences Of The United States Of America 2009, 106: 17933-17938. PMID: 19815526, PMCID: PMC2764927, DOI: 10.1073/pnas.0907766106.Peer-Reviewed Original ResearchConceptsDicer-like enzymesRNA interferenceRNAi pathwayBiogenesis of siRNAsDicer-like proteinsNuclear RNAi pathwaySingle Argonaute proteinEnd modificationReverse genetic analysisRNAi-deficient cellsRNA interference (RNAi) pathwayWild-type cellsAncient eukaryotesArgonaute proteinsRNAi responseAncient traitRepeat transcriptsInterference pathwayCytoplasmic proteinsImportant human pathogenTrypanosoma bruceiGenetic analysisRNAi triggersFunctional analysisIntermediate transcripts