2017
Metacyclic VSG expression site promoters are recognized by the same general transcription factor that is required for RNA polymerase I transcription of bloodstream expression sites
Kolev NG, Günzl A, Tschudi C. Metacyclic VSG expression site promoters are recognized by the same general transcription factor that is required for RNA polymerase I transcription of bloodstream expression sites. Molecular And Biochemical Parasitology 2017, 216: 52-55. PMID: 28716719, PMCID: PMC5582990, DOI: 10.1016/j.molbiopara.2017.07.002.Peer-Reviewed Original ResearchConceptsBloodstream VSG expression sitesVSG expression sitesVSG ES promoterExpression sitesPol IVSG genesES promoterRNA polymerase I transcriptionGeneral transcription factorsBloodstream expression sitesVariant surface glycoprotein (VSG) coatPolymerase I transcriptionRNA polymerase IDistinct promoter sequencesMetacyclic VSG geneTrypanosoma brucei cellsProcyclin genesTypes of promotersBrucei cellsDevelopmental programDistinct binding affinitiesTranscription factorsI transcriptionInitiation factorsPromoter sequences
2015
Genome-wide analysis of small nucleolar RNAs of Leishmania major reveals a rich repertoire of RNAs involved in modification and processing of rRNA
Eliaz D, Doniger T, Tkacz ID, Biswas VK, Gupta SK, Kolev NG, Unger R, Ullu E, Tschudi C, Michaeli S. Genome-wide analysis of small nucleolar RNAs of Leishmania major reveals a rich repertoire of RNAs involved in modification and processing of rRNA. RNA Biology 2015, 12: 1222-1255. PMID: 25970223, PMCID: PMC4829279, DOI: 10.1080/15476286.2015.1038019.Peer-Reviewed Original ResearchConceptsSmall nucleolar RNAsNucleolar RNAsGenome-wide analysisRNA processing mechanismsRRNA secondary structurePost-transcriptional levelCore proteinProcessing of rRNARNA-seq analysisFunctional homologueGene clusterRNA speciesTrypanosoma bruceiSnoRNAsComplete repertoireGene expressionRRNA cleavageAnalogous functionsSecondary structureRNAProtozoan parasiteRich repertoireOrganismsSpeciesProtein
2006
In vivo assembly of functional U7 snRNP requires RNA backbone flexibility within the Sm-binding site
Kolev NG, Steitz JA. In vivo assembly of functional U7 snRNP requires RNA backbone flexibility within the Sm-binding site. Nature Structural & Molecular Biology 2006, 13: 347-353. PMID: 16547514, DOI: 10.1038/nsmb1075.Peer-Reviewed Original Research
2003
VgRBP71 Stimulates Cleavage at a Polyadenylation Signal in Vg1 mRNA, Resulting in the Removal of a cis-Acting Element that Represses Translation
Kolev NG, Huber PW. VgRBP71 Stimulates Cleavage at a Polyadenylation Signal in Vg1 mRNA, Resulting in the Removal of a cis-Acting Element that Represses Translation. Molecular Cell 2003, 11: 745-755. PMID: 12667456, DOI: 10.1016/s1097-2765(03)00071-6.Peer-Reviewed Original Research3' Untranslated RegionsAnimalsBase SequenceBinding SitesBinding, CompetitiveBlotting, WesternGlutathione TransferaseGlycoproteinsMolecular Sequence DataNucleic Acid ConformationOocytesPlasmidsPolymerase Chain ReactionProtein BindingProtein BiosynthesisProtein Structure, SecondaryReverse Transcriptase Polymerase Chain ReactionRibonucleasesRNARNA HelicasesRNA, MessengerRNA-Binding ProteinsTranscription, GeneticTransforming Growth Factor betaXenopusXenopus Proteins