2024
Borg extrachromosomal elements of methane-oxidizing archaea have conserved and expressed genetic repertoires
Schoelmerich M, Ly L, West-Roberts J, Shi L, Shen C, Malvankar N, Taib N, Gribaldo S, Woodcroft B, Schadt C, Al-Shayeb B, Dai X, Mozsary C, Hickey S, He C, Beaulaurier J, Juul S, Sachdeva R, Banfield J. Borg extrachromosomal elements of methane-oxidizing archaea have conserved and expressed genetic repertoires. Nature Communications 2024, 15: 5414. PMID: 38926353, PMCID: PMC11208441, DOI: 10.1038/s41467-024-49548-8.Peer-Reviewed Original ResearchMeSH KeywordsArchaeaDNA MethylationGenome, ArchaealMethaneNanopore SequencingOxidation-ReductionPhylogenySoil MicrobiologyConceptsExtrachromosomal elementsMethylation motifsPublished complete genomesCell surface proteinsShort readsComplete genomeConserved genesGenetic repertoireLinear genomeNanopore sequencingGenomic backboneStructure predictionGenomic signaturesGenomeMethane-oxidizing archaeaHost cellsSurface proteinsArchaeaGenesActivity in situPeatland soilsGenetic inheritanceMotifMethanoperedensHostWidespread extracellular electron transfer pathways for charging microbial cytochrome OmcS nanowires via periplasmic cytochromes PpcABCDE
Portela P, Shipps C, Shen C, Srikanth V, Salgueiro C, Malvankar N. Widespread extracellular electron transfer pathways for charging microbial cytochrome OmcS nanowires via periplasmic cytochromes PpcABCDE. Nature Communications 2024, 15: 2434. PMID: 38509081, PMCID: PMC10954620, DOI: 10.1038/s41467-024-46192-0.Peer-Reviewed Original ResearchConceptsExtracellular electron transferExtracellular electron transfer pathwaysHeme reduction potentialEfficient extracellular electron transferInner membraneBiotechnological applicationsPeriplasmic cytochromesMicrobial nanowiresElectron transfer pathwayPpcABCDEPathwayEET pathwayCytochromePeriplasmEnvironmental processesMicrobesElectron transferBacteriaGeobacterTransfer pathwayReduction potential
2021
Making protons tag along with electrons
Guberman-Pfeffer MJ, Malvankar NS. Making protons tag along with electrons. Biochemical Journal 2021, 478: 4093-4097. PMID: 34871365, DOI: 10.1042/bcj20210592.Peer-Reviewed Original ResearchConceptsExtracellular electron transferProtein engineering strategiesNanowiresBioelectronic applicationsElectron/proton transferEngineering strategiesPeriplasmic cytochromesSoil microbesBioenergetic machineryPili filamentsExtracellular acceptorsGeobacter sulfurreducensElectron transferRecent studiesHarsh environmentsOxidation of nutrientsAliphatic residuesElectron acceptorCellsOMCElectronsFermentationEnergy generationBiofuelsSulfurreducens
2020
Intrinsic electronic conductivity of individual atomically resolved amyloid crystals reveals micrometer-long hole hopping via tyrosines
Shipps C, Kelly HR, Dahl PJ, Yi SM, Vu D, Boyer D, Glynn C, Sawaya MR, Eisenberg D, Batista VS, Malvankar NS. Intrinsic electronic conductivity of individual atomically resolved amyloid crystals reveals micrometer-long hole hopping via tyrosines. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 118: e2014139118. PMID: 33372136, PMCID: PMC7812754, DOI: 10.1073/pnas.2014139118.Peer-Reviewed Original Research
2019
Structure of Microbial Nanowires Reveals Stacked Hemes that Transport Electrons over Micrometers
Wang F, Gu Y, O’Brien J, Yi SM, Yalcin SE, Srikanth V, Shen C, Vu D, Ing NL, Hochbaum AI, Egelman EH, Malvankar NS. Structure of Microbial Nanowires Reveals Stacked Hemes that Transport Electrons over Micrometers. Cell 2019, 177: 361-369.e10. PMID: 30951668, PMCID: PMC6720112, DOI: 10.1016/j.cell.2019.03.029.Peer-Reviewed Original ResearchMeSH KeywordsBiofilmsElectric ConductivityElectron TransportElectronsFimbriae ProteinsFimbriae, BacterialGeobacterHemeNanowiresOxidation-ReductionConceptsMicrobial nanowires
2017
Expressing the Geobacter metallireducens PilA in Geobacter sulfurreducens Yields Pili with Exceptional Conductivity
Tan Y, Adhikari RY, Malvankar NS, Ward JE, Woodard TL, Nevin KP, Lovley DR. Expressing the Geobacter metallireducens PilA in Geobacter sulfurreducens Yields Pili with Exceptional Conductivity. MBio 2017, 8: 10.1128/mbio.02203-16. PMID: 28096491, PMCID: PMC5241403, DOI: 10.1128/mbio.02203-16.Peer-Reviewed Original Research
2015
Functional environmental proteomics: elucidating the role of a c-type cytochrome abundant during uranium bioremediation
Yun J, Malvankar NS, Ueki T, Lovley DR. Functional environmental proteomics: elucidating the role of a c-type cytochrome abundant during uranium bioremediation. The ISME Journal: Multidisciplinary Journal Of Microbial Ecology 2015, 10: 310-320. PMID: 26140532, PMCID: PMC4737924, DOI: 10.1038/ismej.2015.113.Peer-Reviewed Original ResearchConceptsC-type cytochromesGeobacter spOuter-surface c-type cytochromesTight phylogenetic groupDissimilatory metal-reducing microorganismsProteomic studiesPhylogenetic groupsMetal-reducing microorganismsPhylogenetic clustersGenomic DNAFunctional analysisStrain M18Apparent functionClade 1Uranium bioremediationGeobacter sulfurreducensConductive piliGel separationCytochromeGenomeSpBioremediation experimentsCytochrome aPure cultureSubsurface environments