2024
Widespread extracellular electron transfer pathways for charging microbial cytochrome OmcS nanowires via periplasmic cytochromes PpcABCDE
Portela P, Shipps C, Shen C, Srikanth V, Salgueiro C, Malvankar N. Widespread extracellular electron transfer pathways for charging microbial cytochrome OmcS nanowires via periplasmic cytochromes PpcABCDE. Nature Communications 2024, 15: 2434. PMID: 38509081, PMCID: PMC10954620, DOI: 10.1038/s41467-024-46192-0.Peer-Reviewed Original ResearchConceptsExtracellular electron transferExtracellular electron transfer pathwaysHeme reduction potentialEfficient extracellular electron transferInner membraneBiotechnological applicationsPeriplasmic cytochromesMicrobial nanowiresElectron transfer pathwayPpcABCDEPathwayEET pathwayCytochromePeriplasmEnvironmental processesMicrobesElectron transferBacteriaGeobacterTransfer pathwayReduction potential
2023
Structure of Geobacter cytochrome OmcZ identifies mechanism of nanowire assembly and conductivity
Gu Y, Guberman-Pfeffer M, Srikanth V, Shen C, Giska F, Gupta K, Londer Y, Samatey F, Batista V, Malvankar N. Structure of Geobacter cytochrome OmcZ identifies mechanism of nanowire assembly and conductivity. Nature Microbiology 2023, 8: 284-298. PMID: 36732469, PMCID: PMC9999484, DOI: 10.1038/s41564-022-01315-5.Peer-Reviewed Original ResearchConceptsNanowire assembliesHigh electron conductivityExtracellular electron transportNanowire networksExtracellular electron acceptorsG. sulfurreducensElectron conductivityCryogenic electron microscopy structureNanowiresHigh conductivityElectron microscopy structureSerine proteasesDifferent biochemical environmentsElectron transportMicroscopy structureDiverse speciesGeobacter speciesSulfurreducensConductivityImportant bacteriaCharge interactionsElectron acceptorBiochemical environmentSpeciesIdentifies mechanisms
2022
Microbial biofilms as living photoconductors due to ultrafast electron transfer in cytochrome OmcS nanowires
Neu J, Shipps CC, Guberman-Pfeffer MJ, Shen C, Srikanth V, Spies JA, Kirchhofer ND, Yalcin SE, Brudvig GW, Batista VS, Malvankar NS. Microbial biofilms as living photoconductors due to ultrafast electron transfer in cytochrome OmcS nanowires. Nature Communications 2022, 13: 5150. PMID: 36071037, PMCID: PMC9452534, DOI: 10.1038/s41467-022-32659-5.Peer-Reviewed Original ResearchConceptsUltrafast electron transferElectron transferPhotoconductive atomic force microscopyFemtosecond transient absorption spectroscopyQuantum dynamics simulationsMicrobial electron transferAtomic force microscopyTransient absorption spectroscopyValue-added chemicalsIndividual nanowiresWhole-cell catalysisPhotoconductive materialForce microscopyCarrier densityCatalytic performanceNanowiresAbsorption spectroscopyPhotoactive proteinsEfficient productionPhotoconductorsSynthetic photosensitizersDynamics simulationsGeobacter sulfurreducensBiodegradable materialsElectronic interface
2021
Making protons tag along with electrons
Guberman-Pfeffer MJ, Malvankar NS. Making protons tag along with electrons. Biochemical Journal 2021, 478: 4093-4097. PMID: 34871365, DOI: 10.1042/bcj20210592.Peer-Reviewed Original ResearchConceptsExtracellular electron transferProtein engineering strategiesNanowiresBioelectronic applicationsElectron/proton transferEngineering strategiesPeriplasmic cytochromesSoil microbesBioenergetic machineryPili filamentsExtracellular acceptorsGeobacter sulfurreducensElectron transferRecent studiesHarsh environmentsOxidation of nutrientsAliphatic residuesElectron acceptorCellsOMCElectronsFermentationEnergy generationBiofuelsSulfurreducens
2020
Intrinsic electronic conductivity of individual atomically resolved amyloid crystals reveals micrometer-long hole hopping via tyrosines
Shipps C, Kelly HR, Dahl PJ, Yi SM, Vu D, Boyer D, Glynn C, Sawaya MR, Eisenberg D, Batista VS, Malvankar NS. Intrinsic electronic conductivity of individual atomically resolved amyloid crystals reveals micrometer-long hole hopping via tyrosines. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 118: e2014139118. PMID: 33372136, PMCID: PMC7812754, DOI: 10.1073/pnas.2014139118.Peer-Reviewed Original ResearchThe blind men and the filament: Understanding structures and functions of microbial nanowires
Yalcin SE, Malvankar NS. The blind men and the filament: Understanding structures and functions of microbial nanowires. Current Opinion In Chemical Biology 2020, 59: 193-201. PMID: 33070100, PMCID: PMC7736336, DOI: 10.1016/j.cbpa.2020.08.004.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsCytochromesElectron TransportFimbriae, BacterialGeobacterGram-Negative BacteriaModels, MolecularNanowiresConceptsMicrobial nanowiresExtracellular electron transferDifferent nanowiresNanowiresPotential applicationsCryo-electron microscopyTransport electronsMultimodal functional imagingElectron transferBioelectronicsImportant environmental processesRecent discoveryPhysiological studiesOMCApplicationsMicroscopyBiofuelsSensingPhysiological needsMicrometersCrucial roleUnderstanding structureDirect evidenceThousands of papersEnergy production
2019
Structure of Microbial Nanowires Reveals Stacked Hemes that Transport Electrons over Micrometers
Wang F, Gu Y, O’Brien J, Yi SM, Yalcin SE, Srikanth V, Shen C, Vu D, Ing NL, Hochbaum AI, Egelman EH, Malvankar NS. Structure of Microbial Nanowires Reveals Stacked Hemes that Transport Electrons over Micrometers. Cell 2019, 177: 361-369.e10. PMID: 30951668, PMCID: PMC6720112, DOI: 10.1016/j.cell.2019.03.029.Peer-Reviewed Original ResearchMeSH KeywordsBiofilmsElectric ConductivityElectron TransportElectronsFimbriae ProteinsFimbriae, BacterialGeobacterHemeNanowiresOxidation-ReductionConceptsMicrobial nanowires
2017
Expressing the Geobacter metallireducens PilA in Geobacter sulfurreducens Yields Pili with Exceptional Conductivity
Tan Y, Adhikari RY, Malvankar NS, Ward JE, Woodard TL, Nevin KP, Lovley DR. Expressing the Geobacter metallireducens PilA in Geobacter sulfurreducens Yields Pili with Exceptional Conductivity. MBio 2017, 8: 10.1128/mbio.02203-16. PMID: 28096491, PMCID: PMC5241403, DOI: 10.1128/mbio.02203-16.Peer-Reviewed Original Research
2015
Functional environmental proteomics: elucidating the role of a c-type cytochrome abundant during uranium bioremediation
Yun J, Malvankar NS, Ueki T, Lovley DR. Functional environmental proteomics: elucidating the role of a c-type cytochrome abundant during uranium bioremediation. The ISME Journal: Multidisciplinary Journal Of Microbial Ecology 2015, 10: 310-320. PMID: 26140532, PMCID: PMC4737924, DOI: 10.1038/ismej.2015.113.Peer-Reviewed Original ResearchConceptsC-type cytochromesGeobacter spOuter-surface c-type cytochromesTight phylogenetic groupDissimilatory metal-reducing microorganismsProteomic studiesPhylogenetic groupsMetal-reducing microorganismsPhylogenetic clustersGenomic DNAFunctional analysisStrain M18Apparent functionClade 1Uranium bioremediationGeobacter sulfurreducensConductive piliGel separationCytochromeGenomeSpBioremediation experimentsCytochrome aPure cultureSubsurface environments