2022
Protein nanowires with tunable functionality and programmable self-assembly using sequence-controlled synthesis
Shapiro DM, Mandava G, Yalcin SE, Arranz-Gibert P, Dahl PJ, Shipps C, Gu Y, Srikanth V, Salazar-Morales AI, O’Brien J, Vanderschuren K, Vu D, Batista VS, Malvankar NS, Isaacs FJ. Protein nanowires with tunable functionality and programmable self-assembly using sequence-controlled synthesis. Nature Communications 2022, 13: 829. PMID: 35149672, PMCID: PMC8837800, DOI: 10.1038/s41467-022-28206-x.Peer-Reviewed Original ResearchConceptsProtein nanowiresElectronic functionalityChemical-based synthesisConductive protein nanowiresSequence-controlled synthesisHigh electronic conductivityGold nanoparticlesSite-specific conjugationSynthetic chemistryTunable functionalityElectronic conductivityTunable propertiesAttractive biomaterialNonstandard amino acidsSynthetic biologyNanowiresBiomaterialsProtein materialSynthesisAtomic structureMost biomaterialsIncorporation of tryptophanFunctionalityConductivityNanoparticles
2021
Structure of Geobacter pili reveals secretory rather than nanowire behaviour
Gu Y, Srikanth V, Salazar-Morales AI, Jain R, O’Brien J, Yi SM, Soni RK, Samatey FA, Yalcin SE, Malvankar NS. Structure of Geobacter pili reveals secretory rather than nanowire behaviour. Nature 2021, 597: 430-434. PMID: 34471289, PMCID: PMC9127704, DOI: 10.1038/s41586-021-03857-w.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsBiopolymersElectric ConductivityFimbriae ProteinsFimbriae, BacterialGeobacterNanowiresProtein MultimerizationConceptsExtracellular electron transferType 4 piliElectron transferProtein nanowiresCryo-electron microscopyNanowiresNanowire behaviorGeobacter piliC-terminal residuesTranslocation machineryAssembly architectureLoss of secretionMajor phylaGeobacter speciesPrevious structural analysisSurface appendagesGeobacter sulfurreducensAromatic side chainsPiliPilAΠ stackingWidespread effectsBioelectronicsMicroorganismsFilaments
2020
Intrinsic electronic conductivity of individual atomically resolved amyloid crystals reveals micrometer-long hole hopping via tyrosines
Shipps C, Kelly HR, Dahl PJ, Yi SM, Vu D, Boyer D, Glynn C, Sawaya MR, Eisenberg D, Batista VS, Malvankar NS. Intrinsic electronic conductivity of individual atomically resolved amyloid crystals reveals micrometer-long hole hopping via tyrosines. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 118: e2014139118. PMID: 33372136, PMCID: PMC7812754, DOI: 10.1073/pnas.2014139118.Peer-Reviewed Original ResearchElectric field stimulates production of highly conductive microbial OmcZ nanowires
Yalcin SE, O’Brien J, Gu Y, Reiss K, Yi SM, Jain R, Srikanth V, Dahl PJ, Huynh W, Vu D, Acharya A, Chaudhuri S, Varga T, Batista VS, Malvankar NS. Electric field stimulates production of highly conductive microbial OmcZ nanowires. Nature Chemical Biology 2020, 16: 1136-1142. PMID: 32807967, PMCID: PMC7502555, DOI: 10.1038/s41589-020-0623-9.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsElectric ConductivityElectric StimulationElectrophysiological PhenomenaGeobacterNanowiresConceptsConductive protein nanowiresIndividual nanowiresProtein nanowiresElectronic functionalityNanowiresElectric fieldGeobacter sulfurreducens biofilmsHigh conductivityMost natural materialsHigh stiffnessElectrical signalsBidirectional interfaceElectronic systemsLiving materialsNatural materialsΠ stackingSpectroscopic studiesEnergy productionNanospectroscopyConductivityStiffnessConformational switchingΒ-sheetHeme groupSensing
2019
Structure of Microbial Nanowires Reveals Stacked Hemes that Transport Electrons over Micrometers
Wang F, Gu Y, O’Brien J, Yi SM, Yalcin SE, Srikanth V, Shen C, Vu D, Ing NL, Hochbaum AI, Egelman EH, Malvankar NS. Structure of Microbial Nanowires Reveals Stacked Hemes that Transport Electrons over Micrometers. Cell 2019, 177: 361-369.e10. PMID: 30951668, PMCID: PMC6720112, DOI: 10.1016/j.cell.2019.03.029.Peer-Reviewed Original ResearchMeSH KeywordsBiofilmsElectric ConductivityElectron TransportElectronsFimbriae ProteinsFimbriae, BacterialGeobacterHemeNanowiresOxidation-ReductionConceptsMicrobial nanowires
2017
Expressing the Geobacter metallireducens PilA in Geobacter sulfurreducens Yields Pili with Exceptional Conductivity
Tan Y, Adhikari RY, Malvankar NS, Ward JE, Woodard TL, Nevin KP, Lovley DR. Expressing the Geobacter metallireducens PilA in Geobacter sulfurreducens Yields Pili with Exceptional Conductivity. MBio 2017, 8: 10.1128/mbio.02203-16. PMID: 28096491, PMCID: PMC5241403, DOI: 10.1128/mbio.02203-16.Peer-Reviewed Original Research
2016
Reply to 'Measuring conductivity of living Geobacter sulfurreducens biofilms'
Malvankar NS, Rotello VM, Tuominen MT, Lovley DR. Reply to 'Measuring conductivity of living Geobacter sulfurreducens biofilms'. Nature Nanotechnology 2016, 11: 913-914. PMID: 27821844, DOI: 10.1038/nnano.2016.191.Peer-Reviewed Original ResearchLow Energy Atomic Models Suggesting a Pilus Structure that could Account for Electrical Conductivity of Geobacter sulfurreducens Pili
Xiao K, Malvankar NS, Shu C, Martz E, Lovley DR, Sun X. Low Energy Atomic Models Suggesting a Pilus Structure that could Account for Electrical Conductivity of Geobacter sulfurreducens Pili. Scientific Reports 2016, 6: 23385. PMID: 27001169, PMCID: PMC4802205, DOI: 10.1038/srep23385.Peer-Reviewed Original Research