2024
Outer membrane vesicles and the outer membrane protein OmpU govern Vibrio cholerae biofilm matrix assembly
Potapova A, Garvey W, Dahl P, Guo S, Chang Y, Schwechheimer C, Trebino M, Floyd K, Phinney B, Liu J, Malvankar N, Yildiz F. Outer membrane vesicles and the outer membrane protein OmpU govern Vibrio cholerae biofilm matrix assembly. MBio 2024, 15: e03304-23. PMID: 38206049, PMCID: PMC10865864, DOI: 10.1128/mbio.03304-23.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsBiofilmsDNAExtracellular Polymeric Substance MatrixHumansMembrane ProteinsPolysaccharidesProteomicsVibrio choleraeConceptsBiofilm matrix assemblyOuter membrane proteinsOuter membrane protein OmpUOuter membrane vesiclesBiofilm formationBiofilm matrixMatrix assemblyMatrix proteinsBiofilm architectureExtracellular DNAPresence of outer membrane proteinsCausative agent of choleraMembrane vesiclesAgent of choleraBiofilm matrix proteinsBiofilm matrix componentsVibrio cholerae</i>Single-cell force spectroscopyCell surface adhesion forceMatrix proteomeVibrio cholerae</i>.OmpUHuman pathogensMicrobial communitiesEnvironmental survival
2021
Structure of Geobacter pili reveals secretory rather than nanowire behaviour
Gu Y, Srikanth V, Salazar-Morales AI, Jain R, O’Brien J, Yi SM, Soni RK, Samatey FA, Yalcin SE, Malvankar NS. Structure of Geobacter pili reveals secretory rather than nanowire behaviour. Nature 2021, 597: 430-434. PMID: 34471289, PMCID: PMC9127704, DOI: 10.1038/s41586-021-03857-w.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsBiopolymersElectric ConductivityFimbriae ProteinsFimbriae, BacterialGeobacterNanowiresProtein MultimerizationConceptsExtracellular electron transferType 4 piliElectron transferProtein nanowiresCryo-electron microscopyNanowiresNanowire behaviorGeobacter piliC-terminal residuesTranslocation machineryAssembly architectureLoss of secretionMajor phylaGeobacter speciesPrevious structural analysisSurface appendagesGeobacter sulfurreducensAromatic side chainsPiliPilAΠ stackingWidespread effectsBioelectronicsMicroorganismsFilaments
2020
The blind men and the filament: Understanding structures and functions of microbial nanowires
Yalcin SE, Malvankar NS. The blind men and the filament: Understanding structures and functions of microbial nanowires. Current Opinion In Chemical Biology 2020, 59: 193-201. PMID: 33070100, PMCID: PMC7736336, DOI: 10.1016/j.cbpa.2020.08.004.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsCytochromesElectron TransportFimbriae, BacterialGeobacterGram-Negative BacteriaModels, MolecularNanowiresConceptsMicrobial nanowiresExtracellular electron transferDifferent nanowiresNanowiresPotential applicationsCryo-electron microscopyTransport electronsMultimodal functional imagingElectron transferBioelectronicsImportant environmental processesRecent discoveryPhysiological studiesOMCApplicationsMicroscopyBiofuelsSensingPhysiological needsMicrometersCrucial roleUnderstanding structureDirect evidenceThousands of papersEnergy productionElectric field stimulates production of highly conductive microbial OmcZ nanowires
Yalcin SE, O’Brien J, Gu Y, Reiss K, Yi SM, Jain R, Srikanth V, Dahl PJ, Huynh W, Vu D, Acharya A, Chaudhuri S, Varga T, Batista VS, Malvankar NS. Electric field stimulates production of highly conductive microbial OmcZ nanowires. Nature Chemical Biology 2020, 16: 1136-1142. PMID: 32807967, PMCID: PMC7502555, DOI: 10.1038/s41589-020-0623-9.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsElectric ConductivityElectric StimulationElectrophysiological PhenomenaGeobacterNanowiresConceptsConductive protein nanowiresIndividual nanowiresProtein nanowiresElectronic functionalityNanowiresElectric fieldGeobacter sulfurreducens biofilmsHigh conductivityMost natural materialsHigh stiffnessElectrical signalsBidirectional interfaceElectronic systemsLiving materialsNatural materialsΠ stackingSpectroscopic studiesEnergy productionNanospectroscopyConductivityStiffnessConformational switchingΒ-sheetHeme groupSensing
2015
Functional environmental proteomics: elucidating the role of a c-type cytochrome abundant during uranium bioremediation
Yun J, Malvankar NS, Ueki T, Lovley DR. Functional environmental proteomics: elucidating the role of a c-type cytochrome abundant during uranium bioremediation. The ISME Journal: Multidisciplinary Journal Of Microbial Ecology 2015, 10: 310-320. PMID: 26140532, PMCID: PMC4737924, DOI: 10.1038/ismej.2015.113.Peer-Reviewed Original ResearchConceptsC-type cytochromesGeobacter spOuter-surface c-type cytochromesTight phylogenetic groupDissimilatory metal-reducing microorganismsProteomic studiesPhylogenetic groupsMetal-reducing microorganismsPhylogenetic clustersGenomic DNAFunctional analysisStrain M18Apparent functionClade 1Uranium bioremediationGeobacter sulfurreducensConductive piliGel separationCytochromeGenomeSpBioremediation experimentsCytochrome aPure cultureSubsurface environments