A Modular Assembly Platform for Rapid Generation of DNA Constructs
Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, Rideout III W, Papagiannakopoulos T, Xue W, Jacks T. A Modular Assembly Platform for Rapid Generation of DNA Constructs. Scientific Reports 2016, 6: 16836. PMID: 26887506, PMCID: PMC4757859, DOI: 10.1038/srep16836.Peer-Reviewed Original ResearchConceptsAssembly platformDNA constructsInducible lentiviral systemCollection of promotersGeneration of knockTraditional cloning methodsGenetic screenRecombinant DNA technologyRNAi constructsGenomic elementsGenetic toolsSynthetic biologySynthetic promotersDNA fragmentsCloning methodGenetic componentDNA technologyTumor initiationLentiviral systemOne-step productionViral constructsPromoterRapid generationGMAPAssembly