Method for determining whether a gene of Escherichia coli is essential: application to the polA gene
Joyce C, Grindley N. Method for determining whether a gene of Escherichia coli is essential: application to the polA gene. Journal Of Bacteriology 1984, 158: 636-643. PMID: 6233260, PMCID: PMC215477, DOI: 10.1128/jb.158.2.636-643.1984.Peer-Reviewed Original ResearchConceptsPolA geneTarget genesRich mediumEscherichia coliPhage lambda vectorTarget lociBacterial chromosomeHomologous recombinationPhysical mapProphage excisionDNA segmentsMinimal mediumAntibiotic resistance genesPolymerase 3Recombinational eventsLambda vectorGenesResistance genesChromosomal deletionsDeletionFunctional fragmentsExonucleaseMarked deletionPhagesPresence of plasmids