1996
Cis preference of the IS 903 transposase is mediated by a combination of transposase instability and inefficient translation
Derbyshire K, Grindley N. Cis preference of the IS 903 transposase is mediated by a combination of transposase instability and inefficient translation. Molecular Microbiology 1996, 21: 1261-1272. PMID: 8898394, DOI: 10.1111/j.1365-2958.1996.tb02587.x.Peer-Reviewed Original ResearchMeSH KeywordsDNA NucleotidyltransferasesGene Expression RegulationMutationPlasmidsProtein BiosynthesisTranscriptional ActivationTransposasesConceptsClasses of mutationsLevel of transpositionDNA-binding proteinsCis-acting proteinsAmount of transposaseCis preferenceWild-type transposaseInefficient translation initiationSite of synthesisAmino acids 25Translation initiationTranslational initiationTransposase proteinTranslation efficiencyMutant geneGene expressionProtein instabilityTransposase geneInefficient translationProline substitutionTransposaseMutant transposaseMutationsProteinUnusual class
1984
Analysis of the γδ res site Sites required for site-specific recombination and gene expression
Wells R, Grindley N. Analysis of the γδ res site Sites required for site-specific recombination and gene expression. Journal Of Molecular Biology 1984, 179: 667-687. PMID: 6094833, DOI: 10.1016/0022-2836(84)90161-x.Peer-Reviewed Original ResearchMutants of the γδ resolvase: A genetic analysis of the recombination function
Newman B, Grindley N. Mutants of the γδ resolvase: A genetic analysis of the recombination function. Cell 1984, 38: 463-469. PMID: 6088082, DOI: 10.1016/0092-8674(84)90501-4.Peer-Reviewed Original ResearchConceptsAmino acid amino-terminal domainAmino-terminal domainDifferent amino acid substitutionsSite-specific recombinationGamma delta transposonAmino acid substitutionsRecombination functionsTransposon genesIndependent mutantsCatalytic domainRelated recombinasesGenetic analysisDistinct residuesΓδ resolvaseMutantsAcid substitutionsResolvaseRegulatory propertiesCrossover sitesAmino acidsResolvase proteinCointegrate resolutionRecombinasesTransposonGenes
1983
Construction of a plasmid that overproduces the large proteolytic fragment (Klenow fragment) of DNA polymerase I of Escherichia coli.
Joyce C, Grindley N. Construction of a plasmid that overproduces the large proteolytic fragment (Klenow fragment) of DNA polymerase I of Escherichia coli. Proceedings Of The National Academy Of Sciences Of The United States Of America 1983, 80: 1830-1834. PMID: 6340110, PMCID: PMC393703, DOI: 10.1073/pnas.80.7.1830.Peer-Reviewed Original ResearchMeSH KeywordsCloning, MolecularDNA Polymerase IDNA-Directed DNA PolymeraseEscherichia coliGene Expression RegulationLac OperonOperonPeptide FragmentsPlasmidsConceptsDNA polymerase IOverproducing strainPolymerase IGene fusion techniquesLarge proteolytic fragmentCellular proteinsLac promoterGene fragmentsProtein structurePhage lambdaLeftward promoterEscherichia coliCarboxyl terminalPolymerase fragmentProteolytic fragmentsKlenow fragmentPromoterPlasmidPurification procedureFragmentsOverproductionExpressionI. MoreoverMechanistic studiesCloning