1995
DNA transposition: From a black box to a color monitor
Grindley N, Leschziner A. DNA transposition: From a black box to a color monitor. Cell 1995, 83: 1063-1066. PMID: 8548793, DOI: 10.1016/0092-8674(95)90132-9.Peer-Reviewed Original ResearchThe tyrosine‐6 hydroxyl of γδ resolvase is not required for the DNA cleavage and rejoining reactions
Leschziner A, Boocock M, Grindley N. The tyrosine‐6 hydroxyl of γδ resolvase is not required for the DNA cleavage and rejoining reactions. Molecular Microbiology 1995, 15: 865-870. PMID: 7596288, DOI: 10.1111/j.1365-2958.1995.tb02356.x.Peer-Reviewed Original Research
1993
Analysis of a Nucleoprotein Complex: the Synaptosome of γδ Resolvase
Grindley N. Analysis of a Nucleoprotein Complex: the Synaptosome of γδ Resolvase. Science 1993, 262: 738-740. PMID: 8235593, DOI: 10.1126/science.8235593.Peer-Reviewed Original ResearchProtein-protein interactions directing resolvase site-specific recombination: a structure-function analysis.
Hughes R, Rice P, Steitz T, Grindley N. Protein-protein interactions directing resolvase site-specific recombination: a structure-function analysis. The EMBO Journal 1993, 12: 1447-58. PMID: 8385604, PMCID: PMC413356, DOI: 10.1002/j.1460-2075.1993.tb05788.x.Peer-Reviewed Original Research
1991
Gamma delta transposase. Purification and analysis of its interaction with a transposon end
Wiater L, Grindley N. Gamma delta transposase. Purification and analysis of its interaction with a transposon end. Journal Of Biological Chemistry 1991, 266: 1841-1849. PMID: 1846366, DOI: 10.1016/s0021-9258(18)52370-x.Peer-Reviewed Original Research
1990
Integration host factor increases the transpositional immunity conferred by gamma delta ends
Wiater L, Grindley N. Integration host factor increases the transpositional immunity conferred by gamma delta ends. Journal Of Bacteriology 1990, 172: 4951-4958. PMID: 2168370, PMCID: PMC213150, DOI: 10.1128/jb.172.9.4951-4958.1990.Peer-Reviewed Original Research
1989
The 43 residue DNA binding domain of γδ resolvase binds adjacent major and minor grooves of DNA
Rimphanitchayakit V, Hatfull G, Grindley N. The 43 residue DNA binding domain of γδ resolvase binds adjacent major and minor grooves of DNA. Nucleic Acids Research 1989, 17: 1035-1050. PMID: 2537948, PMCID: PMC331720, DOI: 10.1093/nar/17.3.1035.Peer-Reviewed Original Research
1988
Gamma delta transposase and integration host factor bind cooperatively at both ends of gamma delta.
Wiater L, Grindley N. Gamma delta transposase and integration host factor bind cooperatively at both ends of gamma delta. The EMBO Journal 1988, 7: 1907-11. PMID: 2844529, PMCID: PMC457184, DOI: 10.1002/j.1460-2075.1988.tb03024.x.Peer-Reviewed Original Research
1986
Analysis of gamma delta resolvase mutants in vitro: evidence for an interaction between serine-10 of resolvase and site I of res.
Hatfull G, Grindley N. Analysis of gamma delta resolvase mutants in vitro: evidence for an interaction between serine-10 of resolvase and site I of res. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 5429-5433. PMID: 3016704, PMCID: PMC386300, DOI: 10.1073/pnas.83.15.5429.Peer-Reviewed Original ResearchConceptsSerine 10Resolvase mutantsRecombinational activitySite-specific recombination proteinsProtein-DNA complexesPosition 10Site-specific recombinationRecombination proteinsActive site serineMutant proteinsRecombinational crossover pointMutantsResolvaseRecombinational sitesLeucine changeSerineSite ICysteine changeGel electrophoresisSpecific defectsComplex formationCointegrate moleculeTransposon gamma deltaProteinDNA
1984
Mutants of the γδ resolvase: A genetic analysis of the recombination function
Newman B, Grindley N. Mutants of the γδ resolvase: A genetic analysis of the recombination function. Cell 1984, 38: 463-469. PMID: 6088082, DOI: 10.1016/0092-8674(84)90501-4.Peer-Reviewed Original ResearchConceptsAmino acid amino-terminal domainAmino-terminal domainDifferent amino acid substitutionsSite-specific recombinationGamma delta transposonAmino acid substitutionsRecombination functionsTransposon genesIndependent mutantsCatalytic domainRelated recombinasesGenetic analysisDistinct residuesΓδ resolvaseMutantsAcid substitutionsResolvaseRegulatory propertiesCrossover sitesAmino acidsResolvase proteinCointegrate resolutionRecombinasesTransposonGenes
1981
Transposon-mediated site-specific recombination in vitro: DNA cleavage and protein-DNA linkage at the recombination site
Reed R, Grindley N. Transposon-mediated site-specific recombination in vitro: DNA cleavage and protein-DNA linkage at the recombination site. Cell 1981, 25: 721-728. PMID: 6269756, DOI: 10.1016/0092-8674(81)90179-3.Peer-Reviewed Original ResearchConceptsSite-specific recombinationTransposable element gamma deltaDNA cleavageTnpR genePalindromic sequence 5'Intercistronic regionSequence 5DNA moleculesResolution siteResolvaseSame substrate moleculeRecombination sitesAbsence of Mg2Substrate moleculesGenesSame repliconRecombinationDNACleavageTransposonRes sitesSites