2001
The basis of asymmetry in IS2 transposition
Lewis L, Gadura N, Greene M, Saby R, Grindley N. The basis of asymmetry in IS2 transposition. Molecular Microbiology 2001, 42: 887-901. PMID: 11737634, DOI: 10.1046/j.1365-2958.2001.02662.x.Peer-Reviewed Original ResearchDNA Transposable ElementsDNA, BacterialDNA, CircularEscherichia coliMutagenesis, Site-DirectedProtein Structure, TertiaryRepetitive Sequences, Nucleic Acid
1998
Tn552 transposase catalyzes concerted strand transfer in vitro
Leschziner A, Griffin T, Grindley N. Tn552 transposase catalyzes concerted strand transfer in vitro. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 7345-7350. PMID: 9636151, PMCID: PMC22612, DOI: 10.1073/pnas.95.13.7345.Peer-Reviewed Original Research
1995
A functional analysis of the inverted repeat of the gamma delta transposable element.
May E, Grindley N. A functional analysis of the inverted repeat of the gamma delta transposable element. Journal Of Molecular Biology 1995, 247: 578-87. PMID: 7723015, DOI: 10.1006/jmbi.1995.0164.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceBinding SitesDNAGene LibraryMolecular Sequence DataNucleotidyltransferasesPlasmidsPoint MutationProtein BindingRepetitive Sequences, Nucleic AcidTransposasesConceptsIntegration host factorInverted repeatsBase pairsTransposable elementsTransposase bindingGroove contactsIHF siteReduced transposition activityTerminal inverted repeatsMinor groove contactsBase pair regionGamma delta transposaseBase pair stretchSusceptible to mutationsTransposon gamma deltaTn3 familyTransposition activityPoint mutantsTarget plasmidTransposition defectBinding regionMutationsBinding sitesBinding contactsHost factorsA functional analysis of the inverted repeat of the γδ transposable element
May E, Grindley N. A functional analysis of the inverted repeat of the γδ transposable element. Journal Of Molecular Biology 1995, 247: 578-587. DOI: 10.1016/s0022-2836(05)80139-1.Peer-Reviewed Original ResearchBase SequenceBinding SitesDNAGene LibraryMolecular Sequence DataNucleotidyltransferasesPlasmidsPoint MutationProtein BindingRepetitive Sequences, Nucleic AcidTransposases
1992
Binding of the IS903 transposase to its inverted repeat in vitro.
Derbyshire K, Grindley N. Binding of the IS903 transposase to its inverted repeat in vitro. The EMBO Journal 1992, 11: 3449-3455. PMID: 1324175, PMCID: PMC556880, DOI: 10.1002/j.1460-2075.1992.tb05424.x.Peer-Reviewed Original Research
1991
Gamma delta transposase. Purification and analysis of its interaction with a transposon end
Wiater L, Grindley N. Gamma delta transposase. Purification and analysis of its interaction with a transposon end. Journal Of Biological Chemistry 1991, 266: 1841-1849. PMID: 1846366, DOI: 10.1016/s0021-9258(18)52370-x.Peer-Reviewed Original Research
1990
Role of instability in the cis action of the insertion sequence IS903 transposase.
Derbyshire K, Kramer M, Grindley N. Role of instability in the cis action of the insertion sequence IS903 transposase. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 4048-4052. PMID: 2161528, PMCID: PMC54044, DOI: 10.1073/pnas.87.11.4048.Peer-Reviewed Original ResearchMeSH KeywordsDNA Transposable ElementsEscherichia coliGenetic LinkageNucleotidyltransferasesPeptide HydrolasesRecombinant Fusion ProteinsRepetitive Sequences, Nucleic AcidSubstrate SpecificityTransposasesConceptsDNA-binding proteinsCis actionCis-acting proteinsKilobase of DNAInsertion sequenceGalactosidase fusion proteinWild-type transposaseProtease-deficient strainSite of synthesisLon geneBacterial insertion sequencesUnstable proteinTransposase geneFusion proteinTrans actionTransposaseEscherichia coliProteinDefective transposonTransposonGenes
1987
Genetic analysis of the interaction of the insertion sequence IS903 transposase with its terminal inverted repeats.
Derbyshire K, Hwang L, Grindley N. Genetic analysis of the interaction of the insertion sequence IS903 transposase with its terminal inverted repeats. Proceedings Of The National Academy Of Sciences Of The United States Of America 1987, 84: 8049-8053. PMID: 2825175, PMCID: PMC299474, DOI: 10.1073/pnas.84.22.8049.Peer-Reviewed Original ResearchBinding SitesDNA Transposable ElementsDNA, RecombinantEscherichia coliNucleotidyltransferasesRecombination, GeneticRepetitive Sequences, Nucleic AcidTransposases
1983
Insertion Sequence Duplication in Transpositional Recombination
Weinert T, Schaus N, Grindley N. Insertion Sequence Duplication in Transpositional Recombination. Science 1983, 222: 755-765. PMID: 6314502, DOI: 10.1126/science.6314502.Peer-Reviewed Original ResearchBase SequenceChromosome DeletionDNA Transposable ElementsDNA, BacterialPlasmidsRecombination, GeneticRepetitive Sequences, Nucleic Acid
1981
Analysis of the structure and function of the kanamycin-resistance transposon Tn903.
Grindley N, Joyce C. Analysis of the structure and function of the kanamycin-resistance transposon Tn903. Cold Spring Harbor Symposia On Quantitative Biology 1981, 45 Pt 1: 125-33. PMID: 6271455, DOI: 10.1101/sqb.1981.045.01.021.Peer-Reviewed Original Research