2023
IL-22 alters gut microbiota composition and function to increase aryl hydrocarbon receptor activity in mice and humans
Mar J, Ota N, Pokorzynski N, Peng Y, Jaochico A, Sangaraju D, Skippington E, Lekkerkerker A, Rothenberg M, Tan M, Yi T, Keir M. IL-22 alters gut microbiota composition and function to increase aryl hydrocarbon receptor activity in mice and humans. Microbiome 2023, 11: 47. PMID: 36894983, PMCID: PMC9997005, DOI: 10.1186/s40168-023-01486-1.Peer-Reviewed Original ResearchConceptsIL-22 treatmentUC patientsAhR activityIL-22 productionUlcerative colitis patientsGastrointestinal barrier functionMicrobiome compositionGut microbiome compositionAryl hydrocarbon receptorColitis patientsIL-22Gastrointestinal tractHealthy volunteersFunctional capacityMucus secretionAntimicrobial protein productionMicrobiota compositionEpithelial cell differentiationFecal concentrationsReceptor activityGut microbiomePatientsAhR signalingAryl hydrocarbon receptor (AhR) activityBarrier function
2020
The iron-dependent repressor YtgR is a tryptophan-dependent attenuator of the trpRBA operon in Chlamydia trachomatis
Pokorzynski ND, Hatch ND, Ouellette SP, Carabeo RA. The iron-dependent repressor YtgR is a tryptophan-dependent attenuator of the trpRBA operon in Chlamydia trachomatis. Nature Communications 2020, 11: 6430. PMID: 33353937, PMCID: PMC7755916, DOI: 10.1038/s41467-020-20181-5.Peer-Reviewed Original ResearchConceptsIntergenic regionRho-independent transcription terminationOpen reading frameTranscription terminationYtgRAlternative promotersTrp operonOperator sequenceTryptophan limitationOperonMajor promoterModel bacteriaTrpRSPromoterMotifBacteriaExpressionChlamydia trachomatisRepressorTrpBRepressionFundamental importanceTRPLAlternative strategySequence