2020
The iron-dependent repressor YtgR is a tryptophan-dependent attenuator of the trpRBA operon in Chlamydia trachomatis
Pokorzynski ND, Hatch ND, Ouellette SP, Carabeo RA. The iron-dependent repressor YtgR is a tryptophan-dependent attenuator of the trpRBA operon in Chlamydia trachomatis. Nature Communications 2020, 11: 6430. PMID: 33353937, PMCID: PMC7755916, DOI: 10.1038/s41467-020-20181-5.Peer-Reviewed Original ResearchConceptsIntergenic regionRho-independent transcription terminationOpen reading frameTranscription terminationYtgRAlternative promotersTrp operonOperator sequenceTryptophan limitationOperonMajor promoterModel bacteriaTrpRSPromoterMotifBacteriaExpressionChlamydia trachomatisRepressorTrpBRepressionFundamental importanceTRPLAlternative strategySequence
2019
A bipartite iron-dependent transcriptional regulation of the tryptophan salvage pathway in Chlamydia trachomatis
Pokorzynski ND, Brinkworth AJ, Carabeo R. A bipartite iron-dependent transcriptional regulation of the tryptophan salvage pathway in Chlamydia trachomatis. ELife 2019, 8: e42295. PMID: 30938288, PMCID: PMC6504234, DOI: 10.7554/elife.42295.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsChlamydia trachomatisGene Expression Regulation, BacterialIronMetabolic Networks and PathwaysOperonPromoter Regions, GeneticRepressor ProteinsTranscription, GeneticTryptophanConceptsSalvage pathwayNovel promoter elementIron-dependent regulationStress response regulatorGlobal stress response regulatorIron-dependent regulatorTermination of transcriptsIntergenic region upstreamHost immune effectorsResponse regulatorTranscriptional regulationObligate intracellular bacteriumIron starvationIntergenic regionPromoter elementsPromoter upstreamTryptophan biosynthesisStress responseRegion upstreamIron-dependent mechanismEssential nutrientsIntracellular bacteriumChlamydia trachomatisImmune effectorsRegulation
2017
Ironing Out the Unconventional Mechanisms of Iron Acquisition and Gene Regulation in Chlamydia
Pokorzynski ND, Thompson CC, Carabeo RA. Ironing Out the Unconventional Mechanisms of Iron Acquisition and Gene Regulation in Chlamydia. Frontiers In Cellular And Infection Microbiology 2017, 7: 394. PMID: 28951853, PMCID: PMC5599777, DOI: 10.3389/fcimb.2017.00394.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBacterial ProteinsChlamydia InfectionsChlamydia trachomatisGene Expression Regulation, BacterialHomeostasisHost-Pathogen InteractionsHumansIronMammalsPhenotypeTranscription, GeneticConceptsGene regulationIron homeostasisAberrant morphological phenotypesIron biologyUnconventional mechanismObligate intracellular pathogensDeprivation of ironSpecies relativesTranscriptional regulatorsGenome sequenceTransporter subunitsIron acquisitionIron-dependent bacteriaVesicular modeChlamydial speciesMorphological phenotypesIntracellular environmentIntracellular pathogensImportance of ironRecent discoveryAvailability of ironHomeostasisRecent findingsBiologyPersistent phenotype