2024
Metabolic model guided CRISPRi identifies a central role for phosphoglycerate mutase in Chlamydia trachomatis persistence
Chowdhury N, Pokorzynski N, Rucks E, Ouellette S, Carabeo R, Saha R. Metabolic model guided CRISPRi identifies a central role for phosphoglycerate mutase in Chlamydia trachomatis persistence. MSystems 2024, 9: e00717-24. PMID: 38940523, PMCID: PMC11323709, DOI: 10.1128/msystems.00717-24.Peer-Reviewed Original ResearchTranscriptome dataEnzyme costNutrient starvationMetabolic modelsPhosphoglycerate mutaseGenome-scale metabolic modelsResponse to nutrient starvationIron starvation conditionsNutrient-starved conditionsSerovar L2Absence of tryptophanTranscriptional regulationBacterial phenomenonStarvation conditionsNutrient-repleteTranscriptome rewiringCRISPRi knockdownEnzymatic costCRISPRiStress responseTranscriptomeAdaptive response mechanismsNon-replicating formStress conditionsEnzyme
2019
A bipartite iron-dependent transcriptional regulation of the tryptophan salvage pathway in Chlamydia trachomatis
Pokorzynski ND, Brinkworth AJ, Carabeo R. A bipartite iron-dependent transcriptional regulation of the tryptophan salvage pathway in Chlamydia trachomatis. ELife 2019, 8: e42295. PMID: 30938288, PMCID: PMC6504234, DOI: 10.7554/elife.42295.Peer-Reviewed Original ResearchConceptsSalvage pathwayNovel promoter elementIron-dependent regulationStress response regulatorGlobal stress response regulatorIron-dependent regulatorTermination of transcriptsIntergenic region upstreamHost immune effectorsResponse regulatorTranscriptional regulationObligate intracellular bacteriumIron starvationIntergenic regionPromoter elementsPromoter upstreamTryptophan biosynthesisStress responseRegion upstreamIron-dependent mechanismEssential nutrientsIntracellular bacteriumChlamydia trachomatisImmune effectorsRegulation