2023
Bacteria require phase separation for fitness in the mammalian gut
Krypotou E, Townsend G, Gao X, Tachiyama S, Liu J, Pokorzynski N, Goodman A, Groisman E. Bacteria require phase separation for fitness in the mammalian gut. Science 2023, 379: 1149-1156. PMID: 36927025, PMCID: PMC10148683, DOI: 10.1126/science.abn7229.Peer-Reviewed Original ResearchConceptsMammalian gutTranscription termination factor RhoTermination factor RhoGene regulationTranscription terminationMechanisms bacteriaBacteria interactionsHuman commensalValuable targetBacteriaRhoGut microbiotaFitnessNovel clinical applicationsTherapeutic manipulationGutHuman healthCommensalRegulation
2020
The iron-dependent repressor YtgR is a tryptophan-dependent attenuator of the trpRBA operon in Chlamydia trachomatis
Pokorzynski ND, Hatch ND, Ouellette SP, Carabeo RA. The iron-dependent repressor YtgR is a tryptophan-dependent attenuator of the trpRBA operon in Chlamydia trachomatis. Nature Communications 2020, 11: 6430. PMID: 33353937, PMCID: PMC7755916, DOI: 10.1038/s41467-020-20181-5.Peer-Reviewed Original ResearchConceptsIntergenic regionRho-independent transcription terminationOpen reading frameTranscription terminationYtgRAlternative promotersTrp operonOperator sequenceTryptophan limitationOperonMajor promoterModel bacteriaTrpRSPromoterMotifBacteriaExpressionChlamydia trachomatisRepressorTrpBRepressionFundamental importanceTRPLAlternative strategySequence