2024
Metabolic model guided CRISPRi identifies a central role for phosphoglycerate mutase in Chlamydia trachomatis persistence
Chowdhury N, Pokorzynski N, Rucks E, Ouellette S, Carabeo R, Saha R. Metabolic model guided CRISPRi identifies a central role for phosphoglycerate mutase in Chlamydia trachomatis persistence. MSystems 2024, 9: e00717-24. PMID: 38940523, PMCID: PMC11323709, DOI: 10.1128/msystems.00717-24.Peer-Reviewed Original ResearchTranscriptome dataEnzyme costNutrient starvationMetabolic modelsPhosphoglycerate mutaseGenome-scale metabolic modelsResponse to nutrient starvationIron starvation conditionsNutrient-starved conditionsSerovar L2Absence of tryptophanTranscriptional regulationBacterial phenomenonStarvation conditionsNutrient-repleteTranscriptome rewiringCRISPRi knockdownEnzymatic costCRISPRiStress responseTranscriptomeAdaptive response mechanismsNon-replicating formStress conditionsEnzyme
2022
Host Cell Amplification of Nutritional Stress Contributes To Persistence in Chlamydia trachomatis
Pokorzynski N, Alla M, Carabeo R. Host Cell Amplification of Nutritional Stress Contributes To Persistence in Chlamydia trachomatis. MBio 2022, 13: e02719-22. PMID: 36377897, PMCID: PMC9765610, DOI: 10.1128/mbio.02719-22.Peer-Reviewed Original ResearchConceptsHost cell responsesInfected host cellsNutritional stressStress responseIntracellular environmentGTP biosynthesisHost cellsUniversal stress responseTranscriptional stress responseDual RNA sequencingConsequence of coevolutionEssential nutrient ironBacterial stress responseStress response mechanismsChlamydial growthGuanine nucleotide synthesisGenome reductionTryptophan starvationEukaryotic hostsIron starvationInfected epithelial cellsRNA sequencingNutrient ironIron limitationDistinct nutrients
2019
A bipartite iron-dependent transcriptional regulation of the tryptophan salvage pathway in Chlamydia trachomatis
Pokorzynski ND, Brinkworth AJ, Carabeo R. A bipartite iron-dependent transcriptional regulation of the tryptophan salvage pathway in Chlamydia trachomatis. ELife 2019, 8: e42295. PMID: 30938288, PMCID: PMC6504234, DOI: 10.7554/elife.42295.Peer-Reviewed Original ResearchConceptsSalvage pathwayNovel promoter elementIron-dependent regulationStress response regulatorGlobal stress response regulatorIron-dependent regulatorTermination of transcriptsIntergenic region upstreamHost immune effectorsResponse regulatorTranscriptional regulationObligate intracellular bacteriumIron starvationIntergenic regionPromoter elementsPromoter upstreamTryptophan biosynthesisStress responseRegion upstreamIron-dependent mechanismEssential nutrientsIntracellular bacteriumChlamydia trachomatisImmune effectorsRegulation